, 36:388 | Cite as

Biochemical changes in sunflower plants due to seed treatment/spray application with biocontrol agents

  • P. Lamba
  • S. Sharma
  • G. D. Munshi
  • S. K. MunshiEmail author


The application ofTrichoderma harzianum andPseudomonas fluorescens led to increases in dry matter content, starch, total soluble sugars (TSS) and reducing sugar contents in leaves of sunflower (Helianthus annuus) when done as seed treatment or coupled with spray. There was a significant increase in TSS and reducing sugars in stem tissue after treatment with biocontrol agents. The content of total phenols in leaves increased after treatment at 30 and 60 days after sowing (DAS) and at 30 DAS in stem tissues. Biocontrol agents increased the activities of phenylalanine ammonia lyase (PAL) in leaves and stem tissue after 30 DAS and significantly reduced total phenolic content and PAL activity in the stem at 60 DAS. Following treatment with biocontrol agents, seed lipid content increased, the proportion of linoleic acid increased and that of oleic acid decreased. Application of biocontrol agents to sunflower plants initiated certain biochemical changes, which can be considered to be part of the plant’s defense response.

Key words

Biochemical composition Helianthus annuus Pseudomonas fluorescens Trichoderma harzianum 


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Copyright information

© Springer Science + Business Media B.V. 2008

Authors and Affiliations

  • P. Lamba
    • 1
  • S. Sharma
    • 1
  • G. D. Munshi
    • 2
  • S. K. Munshi
    • 1
    Email author
  1. 1.Dept. of Biochemistry and ChemistryPunjab Agricultural UniversityLudhianaIndia
  2. 2.Dept. of Plant PathologyPunjab Agricultural UniversityLudhianaIndia

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