Archives of Pharmacal Research

, 30:1293

Anti-oxidative and photo-protective effects of coumarins isolated fromFraxinus chinensis

  • Bum-Chun Lee
  • So Yong Lee
  • Hwa Jeong Lee
  • Gwan-Sub Sim
  • Jin-Hui Kim
  • Jin-Hwa Kim
  • Young-Ho Cho
  • Dong-Hwan Lee
  • Hyeong-Bae Pyo
  • Tae-Boo Choe
  • Dong Cheul Moon
  • Yeo Pyo Yun
  • Jin Tae Hong
Article

Abstract

Free radicals and reactive oxygen species (ROS), which are generated by UV irradiation, may cause serious injury to skin cell membranes, DNA and functional proteins. In addition, these agents stimulate the expressions of matrix metalloproteinases (MMPs), which can degrade most components of the extracellular matrix (ECM), including collagen. In order to develop new anti-photoaging agents, five major components from the extract ofFraxinus chinensis extract (FCE) were identified. Two of the major components of FCE were found to be esculin (11.2%) and esculetin (1.9%). FCE (IC50: 50.0 μg/mL 1, 1-diphenyl-2-picrylhydrazyl (DPPH); 19.8 μg/mL, Superoxide anion radical) and esculetin (IC50: 2.1 μg/mL DPPH; 0.6 μg/mL, superoxide anion radical) showed strong antioxidative activities. Of the compounds tested, esculetin showed the strongest scavenging activity against DPPH radicals, followed by Superoxide anions from the xanthine/xanthine oxidase system. The intracellular ROS scavenging activity showed that oxidation of 5-(6-)-chloromethyl-2′, 7′-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) was effectively inhibited by esculetin, with potent free radical scavenging activity was also shown in UVB-irradiated human dermal fibroblasts (HDFs). Moreover, treatment of UVA-irradiated HDFs with esculetin resulted in dose-dependent decreases in the expression levels of MMP-1 mRNA and protein. From these results, FCE and one of its components, esculetin, were predicted to be potentially useful as ingredients in cosmetics for protecting against photoaging.

Keywords

Fraxinus chinensis Coumarins Antioxidant MMP-1 Anti-photoaging 

Copyright information

© The Pharmaceutical Society of Korea 2007

Authors and Affiliations

  • Bum-Chun Lee
    • 3
  • So Yong Lee
    • 1
  • Hwa Jeong Lee
    • 1
  • Gwan-Sub Sim
    • 2
  • Jin-Hui Kim
    • 2
  • Jin-Hwa Kim
    • 2
  • Young-Ho Cho
    • 2
  • Dong-Hwan Lee
    • 2
  • Hyeong-Bae Pyo
    • 2
  • Tae-Boo Choe
    • 4
  • Dong Cheul Moon
    • 1
  • Yeo Pyo Yun
    • 1
  • Jin Tae Hong
    • 1
  1. 1.College of PharmacyChungbuk National UniversityCheongju, ChungbukRepbulic of Korea
  2. 2.R&D CenterHanbul Cosmetics Co. Ltd.UmsungKorea
  3. 3.Department of Biochemistry and Molecular Biology, Faculty of Medicine, Tupper Medical BuildingDalhousie UniversityHalifaxCanada
  4. 4.Division of Chemical and Biological EngineeringKonkuk UniversitySeoulKorea

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