Archives of Pharmacal Research

, Volume 26, Issue 11, pp 917–924

Herbal extract prevents bone loss in ovariectomized rats

  • Chungsook Kim
  • Hyekyung Ha
  • Je-Hyun Lee
  • Jin-Sook Kim
  • Kyeyong Song
  • Sie Won Park
Research Articles Articles

Abstract

This research aims to test a new drug candidate based on a traditional medicinal herb, F1, an herbal extract obtained fromAstragalus membranaceus and its main ingredient, 1-monoli-nolein that may have fewer side effects and less uterine hypertrophy.In vitro experiments, human osteoblast-like cell lines, MG-63 and Saos-2, were analyzed by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) and an alkaline phosphatase (ALP) assays. Mouse osteoclasts were induced through a calcium-deficient diet and inhibition effects were measured.In vivo experiments were done using ovariectomized (OVX) rats for 9 weeks. At necropsy, uterus weights were measured, trabecular bone area (TBA) of tibia and lumbar vertebra were measured bone histomorphology. In results, cell proliferation and ALP activity in Saos-2 by ether F1 or 1-monolinolein did not increased significantly compared to the control. The F1 inhibited osteoclast development (IC25= 3.37×10−5 mg/mL) less than 17²-estradiol. The OVX rats administered F1 (2 mg/kg/day and 10 mg/kg/day) showed an increase in TBA of the tibia significantly (136.3±4.2% and 138.5±10.3% of control). In conclusions, the herbal extract, F1 inhibited tibia and lumbar bone loss and did not cause uterine hypertrophy. However, 1-monolinolein, the main ingredient of the herbal extract, did not inhibit bone loss.

Key words

Osteoporosis Herbal extract Osteoblast-like cells Osteoclast Ovariectomized rat Trabecular bone area 

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Copyright information

© The Pharmaceutical Society of Korea 2003

Authors and Affiliations

  • Chungsook Kim
    • 1
  • Hyekyung Ha
    • 1
  • Je-Hyun Lee
    • 1
  • Jin-Sook Kim
    • 1
  • Kyeyong Song
    • 2
  • Sie Won Park
    • 3
  1. 1.Drug Research and Development TeamKorea Institute of Oriental MedicineSeoulKorea
  2. 2.College of MedicineChung-Ang UniversitySeoulKorea
  3. 3.Department of ChemistrySangmyung UniversitySeoulKorea

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