Archives of Pharmacal Research

, Volume 27, Issue 7, pp 742–750

A sphingolipid and tyrosinase inhibitors from the fruiting body ofphellinus linteus

  • Hye Sook Kang
  • Jin Ho Choi
  • Won Ki Cho
  • Jong Cheol Park
  • Jae Sue Choi
Research Articles Articles

Abstract

This paper for the first time reports the isolation of 5 compounds fromPhellinus linteus. A sphingolipid (1) and two tyrosinase inhibitory compounds (2, 3) along with two carboxylic acids (4, 5), were isolated from the fruiting body ofPhellinus linteus (Berk & Curt) Aoshima. The structure of compound 1 was identified as 1-O-β-D-glucopyranosyl-(2S, 3R, 4E, 8E)-2-[(2R)-2-hydroxyhexadecanoylamino]-9-methyl-4,8-octadecadiene-1,3-diol, known as cerebroside B, based on spectroscopic methods such as 1D and 2D NMR as well as by acid hydrolysis. Compounds2~5 were identified as protocatechualdehyde (2), 5-hydroxymethyl-2-furaldehyde (HMF) (3), succinic acid (4), and fumaric acid (5) based on the spectroscopic evidence. Compounds2 and3 inhibited the oxidation of L-tyrosine catalyzed by mushroom tyrosinase with an IC50 of 0.40 and 90.8 μg/mL, respectively. The inhibitory kinetics, which were analyzed by the Lineweaver-Burk plots, were found to be competitive and noncompetitive inhibitors with a Ki of 1.1 μM and 1.4 mM, respectively.

Key words

Phellinus linteus Sphingolipid Cerebroside B Tyrosinase inhibitor 

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Copyright information

© The Pharmaceutical Society of Korea 2004

Authors and Affiliations

  • Hye Sook Kang
    • 1
  • Jin Ho Choi
    • 1
  • Won Ki Cho
    • 1
  • Jong Cheol Park
    • 2
  • Jae Sue Choi
    • 1
  1. 1.Faculty of Food Science and BiotechnologyPukyong National UniversityBusanKorea
  2. 2.Dept. of Oriental Medicine ResourcesSuncheon National UniversitySuncheonKorea

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