Archives of Pharmacal Research

, Volume 27, Issue 7, pp 730–733

Triterpenoid saponin fromviola hondoensis W. Becker et H Boss. and their effect on mmp-1 and type i procollagen expression

Research Articles Articles


Bioassay-guided fractionation has led to the isolation of triterpenoid saponins such as Acutoside A (3-O-[O-β-D-glucopyranosyl-(1→2)-O-β-D-glucopyranosyl] oleanolic acid) from the whole plants ofViola hondoensis. Among them, Saponin 1 exhibited potent inhibitory activity against matrix metalloproteinase (MMP)-1, and prevented the UV-induced changes in the MMP-1 expression. In addition, compound was isolated from this plant for the first time.

Key words

Viola hondoensis Violaceae Triterpenoid saponin MMP1 inhibitor 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bemhard, E. J., Gruber, S. B., and Muschel, R. J., Direct evidence linking expression of matrix metalloproteinase 9 (92-kDa gelatinase/collagenase) to the metastatic phenotype in transformed rat embryo cells.Proc. Nat. Acad. Sci. U.S.A., 91, 4293–4297 (1994).CrossRefGoogle Scholar
  2. Carnat, A. P., Camet, A., Fraisse, D., Lamaison, J. L., Heitz, A., Wylde, R., and Teulade, J. C., Violarvensin, a new flavone di- C-glycoside fromViola arvensis.J. Nat. Prod., 61, 272–274 (1998).PubMedCrossRefGoogle Scholar
  3. Chambers, A. F. and Matrisian, L. M., Changing views of the role of matrix metalloproteinases in metastasis.J. Natl. Cancer Inst., 89, 1260–1270 (1997).PubMedCrossRefGoogle Scholar
  4. Fisher, G. J., Datta, S. C., Taiwan H. S., Wang, Z. Q., Varani, J., Kang, S., and Voorhees, J. J., Molecular basis of sun-induced premature skin ageing and retinoid antagonism.Nature, 379, 335–339 (1996).PubMedCrossRefGoogle Scholar
  5. Fisher, G. J., Wang, Z. Q., Datta, S. C., Varani, J., Kang, S,, and Voorhees, J. J., Pathophysiology of premature skin aging induced by ultraviolet light.Nat. Engl. J. Med., 337, 1419–1428 (1997).CrossRefGoogle Scholar
  6. Gilchrest, B. A., Skin aging and photoaging: an overview.J. Am. Acad. Dermatol., 21, 610–613 (1989).PubMedCrossRefGoogle Scholar
  7. Lee, Y. N., Flora of Korea, Kyohak-Sa, Seoul, Korea. (1996).Google Scholar
  8. Lindholm, P., Goransson, U., Johansson, S., Claeson, P., Gullbo, J., Larsson, R., Bohlin, L., and Backlund, A., Cyclotides: a novel type of cytotoxic agents.Mol. Cancer, Ther., 1, 365–369 (2002).Google Scholar
  9. Mosmann, T., Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.J. Immunol. Methods, 65, 55–63 (1983).PubMedCrossRefGoogle Scholar
  10. Nagao, T., Tanaka, R., Iwase, Y., Hanazono, H., and Okabe, H., Studies on the constituents ofLuffa acutangula Roxb. I. Structures of acutosides A-G, oleanane-type triterpene saponins isolated from the herb.Chem. Pharm. Bull., 39, 599–603 (1991).PubMedGoogle Scholar
  11. Rice-Evans, C. A., Miller, N. J., and Paganga, G., Structure-antioxidant activity relationships of flavonoids and phenolic acids.Free Radical Biol. Med., 20, 933–956 (1996).CrossRefGoogle Scholar
  12. Salah, N., Miller, N. J., Paganga, G., Tijburg, L., Bolwell, G. P., and Rice-Evans, C., Polyphenols flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants.Arch. Biochem. Biophys., 322, 339–346 (1995).PubMedCrossRefGoogle Scholar
  13. Schuele, S. and Lederman, R. J., Long-term outcome of focal dystonia in string instrumentalists.Mov. Disord., 19, 43–48 (2004).PubMedCrossRefGoogle Scholar
  14. Seo. J. Y., Lee. S. H., Youn. C. S., Choi. H. R., Rhie. G. E., Cho. K. H., Kim. K. H., Park. K. C., Eun. H. C., and Chung. J. H., Ultraviolet radiation increases tropoelastin mRNA expression in the epidermis of human skinin vivo.J. Invest. Dermatol., 116, 915–919 (2001).PubMedCrossRefGoogle Scholar
  15. Svangard. E., Goransson. U., Smith. D., Verma. C., Backlund. A., Bohlin. L., and Claeson. P., Primary and 3-D modeled structures of two cyclotides fromViola odorata.Phytochemistry, 64, 135–142 (2003).PubMedCrossRefGoogle Scholar
  16. Tennstedt, D., Comphaut, P., Dooms-Goossens, A., and Lachapelle, J. M., Dermatoses of the affecting violin and viola players.Derm Beruf Umwelt, 27, 165–169 (1979).PubMedGoogle Scholar
  17. Varani, J., Warner, R. L., Gharaee-Kermani, M., Phan, S. H., Kang, S., Chung, J. H., Wang, Z. Q., Datta, S. C., Fisher, G. J., and Voorhees, J. J., Vitamin A antagonizes decreased cell growth and elevated collagen-degrading matrix metalloproteinases and stimulates collagen accumulation in naturally aged human skin.J. Invest. Dermatol., 114, 480–486 (2000).PubMedCrossRefGoogle Scholar
  18. Xie, C., Veitch, N. C., Houghtn, P. J., and Simmonds, M. S., Flavone C-glycosides fromViola yedoensis MAK/NO.Chem. Pharm. Bull., 51, 1204–1207 (2003).PubMedCrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2004

Authors and Affiliations

  1. 1.Department of DermatologySeoul National University College of Medicine, and Clinical research Institute, Seoul National University HosipitalSeoulKorea
  2. 2.Korea Research Institute of Bioscience and BiotechnologyDaejeonKorea
  3. 3.College of PharmacySung Kyun Kwan UniversitySuwonKorea

Personalised recommendations