Archives of Pharmacal Research

, Volume 27, Issue 1, pp 94–98

Effects of dopaminergic drugs on the mast cell degranulation and nitric oxide generation in RAW 264.7 cells

Research Articles Articles

Abstract

Effects of dopaminergic drugs on the degranulation of mast cells (RBL-2H3 cells) and the nitric oxide production from macrophage cells (RAW 264.7) were studied. Among the dopaminergic agonists and antagonists tested, bromocriptine, 7-OH-DPAT, haloperidol, and clozapine showed potent inhibitions of mast cell degranualtion (IC50 value, 5 μM). However, these dopaminergic agents did not affect the tyrosine phosphorylations of the signaling components of the high affinity IgE receptor (FcεRI), such as Syk, PLCγ1, and PLCγ2.; This suggested that these signaling components were not involved in the inhibition of the mast cell degranulation by these compounds. On the other hand, dopamine, bromocriptine, 7-OH-DAPT, and haloperidol markedly inhibited the nitric oxide production from RAW 264.7 cells (IC50 values, 10–20 μM). Bromocriptine, a dopamine agonist that is routinely used for the treatment of Parkinsons disease, inhibited the expression of the inducible nitric oxide synthase at an early stage of the LPS-induced protein expression in a dose-dependent manner. The results suggested that these dopaminergic agents, when used for the treatment of dopamine receptors-related diseases, such as Schizophrenia or Parkinsons disease, might have additional beneficial effects.

Key words

Dopaminergic drugs Mast cell Degranulation Macrophage Nitric oxide 

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Copyright information

© The Pharmaceutical Society of Korea 2004

Authors and Affiliations

  1. 1.Pharmacology Laboratory, College of Pharmacy, Drug Development Research CenterChonnam National UniversityKwang-JuKorea
  2. 2.Pharmacology Laboratory, College of PharmacyChonnam National UniversityKwang-JuKorea

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