, Volume 33, Issue 3, pp 237–244 | Cite as

Resistance to bifenthrin and resistance mechanisms of different strains of the two-spotted spider mite (Tetranychus urticae) from Turkey



Nine different strains of the two-spotted spider miteTetranychus urticae Koch (Acari: Tetranychidae) were collected on cotton from Adana, Antalya, Izmir, Manisa and Urfa in Turkey. Their responses to bifenthrin were investigated using conventional bioassay and biochemical assays. LC50 and LC90 values of bifenthrin were determined for all strains by using a residual bioassay with a petri dish-spray tower. Resistance ratios were determined by comparing the samples with a standard susceptible strain, GSS. The resistance ratios of the strains ranged from <1 to 669-fold (at LC50). Of the investigated field strains, only three (two from Adana and one from Urfa) were resistant to bifenthrin. There was a correlation between esterase enzyme activity and bifenthrin resistance according to polyacrylamide gel electrophoresis and microtiter plate assays in the three resistant strains.

Key words

Tetranychus urticae two-spotted spider mite resistance bifenthrin esterase cotton 


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Copyright information

© Springer Science + Business Media B.V. 2005

Authors and Affiliations

  1. 1.Plant Protection Department, Faculty of AgricultureSüleyman Demirel UniversityIspartaTurkey
  2. 2.Plant Protection Department, Faculty of AgricultureAnkara UniversityAnkaraTurkey

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