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Phytoparasitica

, Volume 32, Issue 3, pp 286–294 | Cite as

Effect of azadirachtin applied systemically through roots of plants on the mortality, development and fecundity of the cabbage aphid (Brevicoryne brassicae)

  • Roman PavelaEmail author
  • Martin Barnet
  • František Kocourek
Entomology

Abstract

The effect of low concentrations of azadirachtin applied systemically through root tissues of rape plants (Brassica napus subsp.napus) on the mortality, period of development, longevity and fecundity of the cabbage aphid (Brevicoryne brassicae L.) (Sternorrhyncha: Aphididae) was studied. The concentrations of azadirachtin A tested were: 0.25, 0.05, 0.005, 0.0005 and 0.00005 mg ml−1. The mortality of cabbage aphid nymphs, particularly during the period of ecdysis, increased significantly with increasing concentrations. Azadirachtin had no effect on the length of the development period of the respective immature stages of cabbage aphids. The average longevity of the cabbage aphids decreased with increasing azadirachtin concentration and time spent feeding. The fecundity of aphids decreased after the application of azadirachtin.

Key words

Azadirachtin systemic action Brevicoryne brassicae cabbage aphids fecundity mortality botanical insecticides terpenoids 

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References

  1. 1.
    Basedow, T., Ossiewatsch, H.R., Bernal Vega, J.A., Kollmann, S., El-Shafie, H.A.F. and Nicol, C.M.Y. (2002) Control of aphids and whiteflies (Homoptera: Aphididae and Aleyrodidae) with different Neem preparations in laboratory, greenhouse and field: effects and limitations.Z. Pflanzenkr. Pflanzenschutz 109:612–623.Google Scholar
  2. 2.
    Cohen, E., Quistad, G.B. and Casida, J.E. (1996) Cytotoxicity of nimbolide, epoxyazadiradione and other limonoids from neem insecticide.Life Sci. 58:1075–1081.PubMedCrossRefGoogle Scholar
  3. 3.
    Cohen, E., Quistad, G.B., Jefferies, P.R. and Casida, J.E. (1996) Nimbolide is the principal cytotoxic component of Neem-Seed insecticide preparations.Pestic. Sci. 48:135–140.CrossRefGoogle Scholar
  4. 4.
    Dimetry, N. and Schmidt, G.H. (1992) Efficacy of Neem-Azal S and Margosan-O against the bean aphidAphis fabae Scop.Anz. Schaedl. Pflanzenschutz Umweltschutz 65:75–79.CrossRefGoogle Scholar
  5. 5.
    Dimetry, N.Z. and El-Hawary, F.M.A. (1995) Neem Azal-F as an inhibitor of growth and reproduction in the cowpea aphidAphis craccivora Koch.J. Appl. Entomol. 119:67–71.CrossRefGoogle Scholar
  6. 6.
    Gelbic, I., Tonner, M. and Hol, A. (1984) Aphid sterility induced by antiviral agent (RS)-9-(2,3 dihydroxypropyl) adenine.Acta Entomol. Bohemoslov. 81:46–53.Google Scholar
  7. 7.
    Helson, B.V., Lyons, B.D., Wanker, K.W. and Scarr, T.A. (2001) Control of conifer defoliators with neem-based systemic bioinsecticides using a novel injection device.Can. Entomol. 133:729–744.Google Scholar
  8. 8.
    Isman, M.B. (1999) Neem and related natural products.in: Hall, F.R. and Menn, J.J. [Eds.] Biopesticides: Use and Delivery. Humana, Totowa, NJ, USA. pp. 139–153.Google Scholar
  9. 9.
    Koul, O. (1996) Neem research and development: present and future scenario.in: Handa, S.S. and Koul, M.K. [Eds.] Supplement to Cultivation and Utilization of Medicinal Plants. PID CSIR, New Delhi, India. pp. 583–611.Google Scholar
  10. 10.
    Koul, O. (1998) Effect of neem extract and azadirachtin on fertility and fecundity of cabbage aphid,Brevicoryne brassicae (L.).Pestic. Res. J. 10:258–261.Google Scholar
  11. 11.
    Koul, O. (1999) Insect growth regulating and antifeedant effects of neem extracts and azadirachtin on two aphid species of ornamental plants.J. Biosci. 24:85–90.CrossRefGoogle Scholar
  12. 12.
    Lowery, D.T. and Isman, M.B. (1994) Insect growth regulating effects of neem extract and azadirachtin on aphids.Entomol. Exp. Appl. 72:77–84.CrossRefGoogle Scholar
  13. 13.
    Lowery, D.T. and Isman, M.B. (1995) Toxicity of neem to natural enemies of aphids.Phytoparasitica 23:297–306.Google Scholar
  14. 14.
    Lowery, D.T. and Isman, M.B. (1996) Inhibition of aphid (Homoptera: Aphididae) reproduction by neem seed oil and azadirachtin.J. Econ. Entomol. 89:602–607.Google Scholar
  15. 15.
    Mordue, A.J. and Blackwell, A. (1993) Azadirachtin: an update.J. Insect Physiol. 39:903–924.CrossRefGoogle Scholar
  16. 16.
    Mordue (Luntz), A.J., Simmonds, M.S.J., Ley, S.V., Blaney, W.M., Mordue, W., Nasiruddin, M.et al. (1998) Action of azadirachtin, a plant allelochemical, against insects.Pestic. Sci. 54:277–284.CrossRefGoogle Scholar
  17. 17.
    Naumann, K. and Isman, M.B. (1996) Toxicity of neem (Azadirachta indica A. Juss.) seed extracts to larval honeybees and estimation of dangers from field application.Am. Bee J. 136:518–520.Google Scholar
  18. 18.
    Naumann, K., Rankin, L.J. and Isman, M.B., (1994) Systemic action of neem seed extract on mountain pine beetle (Coleoptera: Scolytidae) in lodgepole pine.J. Econ. Entomol. 87:1580–1585.Google Scholar
  19. 19.
    Nisbet, A.J., Woodfort, J.A.T. and Strang, R.H. (1994) The effects of azadirachtin-treated diets on the feeding behaviour and fecundity of the peach-potato aphid,Myzus persicae.Entomol. Exp. Appl. 71:65–72.CrossRefGoogle Scholar
  20. 20.
    Opender, K., Shankar, J.S. and Koul, O. (1995) Systemic uptake of azadirachtin intoRicinus communis and its effects onSpodoptera litura larvae.Indian J. Exp. Biol. 33:865–867.Google Scholar
  21. 21.
    Pavela, R., Barnet, M., Belanger, A. and Brosseau, M. (2002) Effectiveness of new plant insecticides obtained from neem-tree (Azadirachta indica Juss.) against cabbage aphid (Brevicoryne brassicae).Veg. Crops Res. Bull. 56:95–102.Google Scholar
  22. 22.
    Pavela, R. and Holy, K. (2003) Effects of azadirachtin on larvae ofLymantria dispar, Spodoptera littoralis andMamestra brassicae.Hortic. Veg. Growing 22:434–441.Google Scholar
  23. 23.
    Stark, J.D. and Rangus, T.M. (1994) Lethal and sublethal effects of the Neem insecticide formulation, ‘Margosan-O’, on the pea aphid.Pestic. Sci. 41:155–160.CrossRefGoogle Scholar
  24. 24.
    Sundaram, K.M.S. (1996) Root uptake, translocation, accumulation and dissipation of the botanical insecticide, azadirachtin, in young spruce trees.J. Environ. Sci. Health Part B Pestic. Food Contam. Agric. Wastes 31:1289–1306.Google Scholar
  25. 25.
    Walter, J.F. (1999) Commercial experience with neem products.in: Hall, F.R. and Menn, J.J. [Eds.] Biopesticides: Use and Delivery. Humana, Totowa, NJ, USA. pp. 155–170.Google Scholar
  26. 26.
    Wanner, K.W., Helson, B.V. and Kostyk, B.C. (1997) Foliar and systemic applications of neem seed extract for control of spruce budworm,Choristoneura fumifera (Clem.) (Lepidoptera: Tortricidae), infesting black and white spruce seed orchards.Can. Entomol. 129:645–655.CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media B.V. 2004

Authors and Affiliations

  • Roman Pavela
    • 1
    Email author
  • Martin Barnet
    • 1
  • František Kocourek
    • 1
  1. 1.Research Institute of Crop ProductionPraha 6-RuzyneCzech Republic

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