Archives of Pharmacal Research

, Volume 18, Issue 3, pp 173–178 | Cite as

Ciprofloxacin resistance by altered gyrase and drug efflux system inPseudomonas aeruginosa

  • Myungsun Cho
  • Doyeob Kim
  • Jae Yang Kong
  • Sung-II Yang
Research Articles
Received:

Abstract

Ciprofloxacin resistance mechanisms were studied by investigating the inhibitory effect of ciprofloxacin on the gyrase-mediated DNA supercoiling and the intracellular accumulation of ciprofloxacin in clinical isolates ofPseudomonas aeruginosa. A higher amount of ciprofloxacin was required to inhibit the gyrases purified from the ciprofloxacin-resistant strains than that from the sensitive strain. Reconstitution of heterologous gyrase subunits from different strains revealed alterations in the A and/or the B subunits of gyrase in these strains. In addition, the resistant strains accumulated approximately a half amount of ciprofloxacin inside the cells, compared to the sensitive strain. However, when the active efflux was blocked by carbonyl cyanide m-chlorophenyl hydrazone treatment, intracellular concentration of ciprofloxacin was elevated about 4–7 fold in these strains, while the sensitive strain was not significantly affected by this treatment, indicating that the ciprofloxacin-resistant strains developed a drug efflux system. Interestingly, these resistant strains expressed an envelope protein of approximately 51 kD. These studies suggest that alterations in the gyrase as well as the active drug-efflux system conferred dual ciprofloxacin resistance mechanisms to these clinical isolates ofP. aeruginosa.

Key words

Ciprofloxacin Resistance Gyrase Efflux Pseudomonas aeruginosa 
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Copyright information

© The Pharmaceutical Society of Korea 1995

Authors and Affiliations

  • Myungsun Cho
    • 1
  • Doyeob Kim
    • 1
  • Jae Yang Kong
    • 1
  • Sung-II Yang
    • 1
  1. 1.Pharmaceutical Screeing CenterKorea Research Institute of Chemical TechnologyTaeJonKorea

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