Archives of Pharmacal Research

, 28:1376 | Cite as

Hepatoprotective constituents of the edible brown algaEcklonia stolonifera on tacrine-induced cytotoxicity in hep G2 cells

  • Youn Chul Kim
  • Ren Bo An
  • Na Young Yoon
  • Taek Jeong Nam
  • Jae Sue ChoiEmail author
Articles Drug Development


In this study, ethanolic extracts from 18 seaweed variants were assessed for hepatoprotective activity against tacrine-induced cytotoxicity in Hep G2 cells. Only one of these,Ecklonia stolonifera Okamura (Laminariaceae), a member of the brown algae, exhibited promising hepatoprotective activity. Bioassay-guided fractionation of the active ethyl acetate (EtOAc) soluble fraction obtained from the ethanolic extract ofE. stolonifera, resulted in the isolation of several phlorotannins [phloroglucinol (1), eckstolonol (2), eckol (3), phlorofucofuroeckol A (4), and dieckol (5)]. Compounds 2 and 4 were determined to protect Hep G2 cells against the cytotoxic effects of tacrine, with EC50 values of 62.0 and 79.2 μg/mL, respectively. Silybin, a well characterized hepatoprotective agent, was used as a positive control, and exhibited an EC50 value of 50.0 μg/mL. It has been suggested that the phlorotannins derived from marine brown algae might prove useful sources in the development of novel hepatoprotective agents.

Key words

Ecklonia stolonifera Hepatoprotective Phlorotannins Marine algae Tacrine Hep G2 cells 


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Copyright information

© The Pharmaceutical Society of Korea 2005

Authors and Affiliations

  • Youn Chul Kim
    • 3
  • Ren Bo An
    • 1
  • Na Young Yoon
    • 2
  • Taek Jeong Nam
    • 2
  • Jae Sue Choi
    • 2
    Email author
  1. 1.College of PharmacyYanbian UniversityYanji, JilinChina
  2. 2.Faculty of Food Science and BiotechnologyPukyong National UniversityBusanKorea
  3. 3.College of PharmacyWonkwang UniversityIksanKorea

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