Determination of water content in skin by using a ft near infrared spectrometer

Drug efficacy

Abstract

The water content of skin was determined using a FT near infrared (NIR) spectrometer. NIR diffuse reflectance spectra were collected from hairless mouse,in vitro, and from human inner arm,in vivo. It was found that the variation of NIR absorbance band 1450 nm from OH vibration of water and 1940 nm from the combination involving OH stretching and OH deformation, depending on the absolute water content of separated hairless mouse skin,in vitro, using the FT NIR spectrometer. Partial least squares regression (PLSR) was applied to develop a calibration model. The PLS model showed good correlation. For practical use of the evaluation of human skin moisture, the PLS model for human skin moisture was developedin vivo on the basis of the relative water content of stratum corneum from the conventional capacitance method. The PLS model predicted human skin moisture with a standard errors of prediction (SEP) of 3.98 at 1130-1830 nm range. These studies showed the possibility of a rapid and nondestructive skin moisture measurement using FT NIR spectrometer.

Key words

FT near infrared (NIR) Skin moisture 

References

  1. Caspers, P. J., Lucassen, G. W., Carter, E. A., Bruining, H. A., and Puppets, G. J.,In-vivo confocal raman microscopy of the skin: Non-invasive determination of molecular concentration profiles.J. Invest. Dermatol., 116, 434–442 (2001).PubMedCrossRefGoogle Scholar
  2. Courage W., Hardware and measuring principle: Corneometer, In Elsner, P., Berardesca, E., and Maibach, H. I. (Eds.). Bioengineering of the skin: Water and the Stratum Corneum. CRC press, Boca Raton, Chapter 14, (1994).Google Scholar
  3. Egawa, M., Fukuhara, T., Takahashi, M., and Ozaki, Y., Determining Water Content in Human Nails with a Portable Near-Infrared Spectrometer.Appl. Spectrosc., 57, 473–478 (2003).PubMedCrossRefGoogle Scholar
  4. Heise, H. M., Clinical applications of near-and mid-infrared spectroscopy, In Gremlich, H. U., and Yan, B. (Eds.). Infrared and raman spectroscopy of biological materials. Marcel Dekker, New York, (2000).Google Scholar
  5. Kumar, G. and Shmitt, J. M., Optimal probe geometry for near-infrared spectroscopy of biological tissue.Appl. Optics, 36, 2286–2293 (1997).CrossRefGoogle Scholar
  6. Libnau, F. O., Kvalheim, O. M., Christy, A. A., and Toft, J., Infrared spectra of water in the NIR and MIR region.Vib. Spectrosc., 7, 243–254 (1994).CrossRefGoogle Scholar
  7. Lucassen, G. W., Veen, G. N. A., and Jansen, J. A. J., Band analysis of hydrated human skin stratum corneum attenuated total reflectance fourier transform intrared spectrain vivo. J. Biomedical Optics, 3, 267–280 (1998).CrossRefGoogle Scholar
  8. Martin, K. A., Direct measurement of moisture in skin by NIR spectroscopy.J. Soc. Cosmet. Chem., 44, 249–261 (1993).Google Scholar
  9. Martin, K.,In vivo measurements of water in skin by near-infrared reflectance.Appl. Spectrosc., 52, 1001–1007 (1998).CrossRefGoogle Scholar
  10. Martinsen, O. G., Grimnes, S., and Karlsen, J., Electrical methods for skin moisture assessment.Skin Pharmacol., 8, 237–245 (1995).PubMedCrossRefGoogle Scholar
  11. Obata, M. and Tagami, H., A rapidin vitro test to assess skin moisturizers.J. Soc. Cosmet. Chem., 41, 235–241 (1990).Google Scholar
  12. Potts, R. O., Stratum corneum hydration: Experimental techniques and interpretations of results.J. Soc. Cosmet. Chem., 37, 9–33 (1986).Google Scholar
  13. Prasch, T. H., Knübel, G., Schmidt-Fonk, K., Ortanderl, S., Nieveler, S., and Förster, T. H., Infrared spectroscopy of the skin: influencing the stratum corneum with cosmetic products.Int. J. Cos. Sci., 22, 371–383 (2000).CrossRefGoogle Scholar
  14. Rigal, J., Losch, M. J., Bazin, R., Camus, C., Sturelle, C., Descamps, V., and Leveque, J. L., Near-infrared spectroscopy: A new approach to the characterization of dry skin.J. Soc. Cosmet. Chem., 44, 197–209 (1993).Google Scholar
  15. Salter, D. C., Examination of stratum corneum hydration state by electrical methods.Curr. Probl. Dermatol., 26, 38–47 (1998).PubMedCrossRefGoogle Scholar
  16. Sowa, M. G., Payette, J. R., and Mantsch, H. H., Near-infrared spectroscopic assessment of tissue hydration following surgery.J. Surg. Res., 86, 62–69 (1999).PubMedCrossRefGoogle Scholar
  17. Tagami, H., Quantitative measurements of water concentration of the stratumcorneum in vivo by high-frequency current.Acta Derm. Venerol. Suppl. (Stockh.), 185, 29–33 (1994).Google Scholar
  18. Walling, P. L. and Dabney, J. M., Moisture in skin by near-infrared reflectance spectroscopy.J. Soc. Cosmet. Chem., 40, 151–171 (1989).Google Scholar
  19. Wichrowski, K., Sore, G., and Khaïat, A., Use of infrared spectroscopy forin vivo measurement of the stratum corneum moisturization after application of cosmetic preparations.Int. J. Cos. Sci., 17, 1–11 (1995).CrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2005

Authors and Affiliations

  1. 1.College of PharmacyDongduk Women’s UniversitySeoulKorea

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