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Archives of Pharmacal Research

, Volume 30, Issue 3, pp 361–365 | Cite as

Isolation of fatty acids with anticancer activity fromProtaetia brevitarsis Larva

  • Yung-Choon Yoo
  • Byung-Hoon Shin
  • Jang-Hee Hong
  • Junglim Lee
  • Hee-Youn Chee
  • Kyung-Sik Song
  • Kyung-Bok Lee
Article

Abstract

In this study, biologically active compounds were isolated fromProtaetia brevitarsis larva (PBL) by dichloromethane extraction. The dichloromethane extract from PBL was highly cytotoxic to various cancer cells. From a silica gel column chromatograpy of this extract, we obtained four fractions (F-2, F-4, F-5 and F-7) having apoptosis-inducing activity. These fractions induced DNA ladder and caspase-3 activation during apoptosis in colon 26 tumor cells. In1H and13C NMR and mass spectral analysis of the fraction F-2 showing the highest apoptosis-inducing activity, we found that the fraction was composed of three free fatty acids such as palmitic acid, (Z)-9-octadecenoic acid and octadecenoic acid. These results indicate that the dichloromethane extract of PBL includes anticancer components composed of at least three fatty acids, and apoptosis-inducing activity of the extract was mediated by caspase-3 activation in tumor cells.

Key words

Protaetia brevitarsis Apoptosis Caspase-3 Fatty acid 

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Referrences

  1. Abdullaev, F. I. and Espinosa-Aguirre, J. J., Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials.Cancer Detect Prev., 28, 426–432 (2004).PubMedCrossRefGoogle Scholar
  2. Andrade, L. N., de Lima, T. M., Curi, R., and Castrucci, A. M., Toxicity of fatty acids on murine and human melanoma cell lines.Toxicol. In vitro, 19, 553–660 (2005).PubMedCrossRefGoogle Scholar
  3. Boman, H. C., Peptide antibiotics and their role in innate immunity.Annu. Rev. Immunol., 13, 61–92 (1995).PubMedCrossRefGoogle Scholar
  4. Cai, Z., Xu, C, Xu, Y, Lu, W., Chi, C. W., Shi, Y, and Wu, J., Solution structure of BmBKTx1, a new BKCal channel blocker from the Chinese scorpionButhus martensi Karsch.Biochem., 43, 3764–3771 (2004).CrossRefGoogle Scholar
  5. Cozzi, P., Mongelli, N., and Suarato, A., Recent anticancer cytotoxic agents.Curr Med Chem Anti-cancer Agents, 4, 93–121 (2004).CrossRefGoogle Scholar
  6. Farkya, S., Bisaria, V. S., and Srivastava, A. K., Biotechnologi- cal aspects of the production of the anticancer drug podo- phyllotoxin.Appl Microbiol Biotechnol., 65, 504–519 (2004).PubMedCrossRefGoogle Scholar
  7. Fu, Y. J., Chai, B. F., Wang, W., Zhi, H., Yin, L.T., and Liang, A. H., Expression and purification of the BmK Mm2 neurotoxin from the scorpionButhus martensii Karsch and its biological activity test.Protein. Expr. Purif., 38, 45–50 (2004).PubMedCrossRefGoogle Scholar
  8. Goo, T. W., Yun, E. Y, Choi, K. H., Kim, S. H., Nho, S. K., Kang, S. W., and Kwon, O. Y., ATFC is a novel transducer for the unfolded protein response inBombyx mori BM5 cells.Biochem. Biophys. Res. Commun., 325, 626–631 (2004).PubMedCrossRefGoogle Scholar
  9. Kong, J. Y. and Rabkin, S. W., Lovastatin does not accentuate but is rather additive to palmitate-induced apoptosis in cardiomyocytes.Prostaglandins Leukot. Essent. Fatty Acids, 67, 293–302 (2002).PubMedCrossRefGoogle Scholar
  10. Kostova, I., Synthetic and natural coumarins as cytotoxic agents.Curr Med Chem Anti-Canc Agents, 5, 29–46 (2005).CrossRefGoogle Scholar
  11. Lee, D. G., Park, J. H., Lee, S. G, Kim, M. K., and Hahm, K. S., Design of novel analogue peptides with potent fungicidal but low hemolytic activity based on the cecrapin A-mellitin hybrid structure.Biochem. Mol. Biol. Int., 43, 489–498 (1997).PubMedGoogle Scholar
  12. Palik, E. D., Handbook of proton-NMR spectra and data. Asahi Research Center, Japan (1985).Google Scholar
  13. Park, C. W., Kim, J. H., Kim, K. M., Hwang, J. S., Kang, S. W., Kang, H. S., Cho, B. P, Yu, C. H., Kim, H. R., and Lee, B. H., Evidence for brain-derived neurotrophic factor-like neurope- ptide in brain of the silk mothBombyx mori during postem- bryonic periods.Peptides, 25, 1891–1897 (2004).PubMedCrossRefGoogle Scholar
  14. Tanaka, R., Noda, N., and Miyahara, K., Glycerophosphocholines of the earthworm, Pheretima asiatica.Chem. Pharm. Bull. (Tokyo), 47, 1167–1169 (1999).Google Scholar
  15. Tettamanti, G., Grimaldi, A., Rinaldi, L., Amaboldi, F., Congiu, T., Valvassori, R., and de Eguileor, M., The multifunctional role of fibroblasts during wound healing in Hirudo medicinalis (Annelida, Hirudinea).Biol. Cell., 96, 443–455 (2004).PubMedCrossRefGoogle Scholar
  16. Tossi, A., Tarantino, C, and Romeo, D., Design of synthetic antimicrobial peptides based on sequence analogy and amphipathicity.Eur. J. Biochem., 250, 549–558 (1997).PubMedCrossRefGoogle Scholar
  17. Yang, L. L, Wang, M. C., Chen, L. G., and Wang, C. C., Cytotoxic activity of coumarins from the fruits ofCuidium monnieri on leukemia cell lines.Planta Med., 69, 1091–1095 (2003).PubMedCrossRefGoogle Scholar
  18. Yoo, Y. C., Watanabe, R., Koike, Y., Mitobe, M., Shimazaki, K., Watanabe, S., and Azuma, I., Apoptosis in human leukemic cells induced by lactoferricin, a bovine milk protein-derived peptide: involvement of reactive oxygen species.Biochem. Biophys. Res. Commun., 237, 624–628 (1997).PubMedCrossRefGoogle Scholar
  19. Yoon, H. S., Lee, C. S., Lee, S. Y, Choi, C. S., Lee, I. H., Yeo, S. M., and Kim, H. R., Purification and cDNA cloning of inducible antibacterial peptides fromProtaetia brevitarsis.Arch. Insect. Biochem. Physiol., 52, 92–103 (2003).PubMedCrossRefGoogle Scholar
  20. You, W. K., Sohn, Y D., Kim, K. Y, Park, D. H., Jang, Y., and Chung, K. H., Purification and molecular cloning of a novel serine protease from the centipede,Scolopendre subspinipes mutilans.Insect. Biochem. Mol. Biol., 34, 239–250 (2004).PubMedCrossRefGoogle Scholar
  21. Vergote, D., Sautiere, P. E., Vandenbulcke, F., Vieau, D., Mitta, G, Macagno, E. R., and Salzet, M., Up-regulation of neuro- hemerythrin expression in the central nervous system of the medicinal leech,Hirudo medicinalis, following septic injury.J. Biol. Chem., 279, 43828–43837 (2004).PubMedCrossRefGoogle Scholar
  22. Xu, C. Q., Brone, B., Wicher, D., Bozkurt, O., Lu, W. Y, Huys, I., Han, Y. H., Tytgat, J., Van Kerkhove, E., and Chi, C. W., BmBKTx1, a novel Ca2+-activated K+ channel blocker purified from the Asian scorpionButhus martensi Karsch.J. Biol. Chem., 279, 34562–34569 (2004).PubMedCrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2007

Authors and Affiliations

  • Yung-Choon Yoo
    • 3
  • Byung-Hoon Shin
    • 3
  • Jang-Hee Hong
    • 4
  • Junglim Lee
    • 1
  • Hee-Youn Chee
    • 2
  • Kyung-Sik Song
    • 2
  • Kyung-Bok Lee
    • 4
  1. 1.Department of Microbiology, College of MedicineKonyang UniversityDaejeonKorea
  2. 2.Department of Cell Biology, College of MedicineKonyang UniversityDaejeonKorea
  3. 3.Division of Applied Biology and Chemistry, College of Agriculture and Life SciencesKyungpook National UniversityDaeguKorea
  4. 4.Department of Biochemistry, College of MedicineKonyang UniversityDaejeonKorea

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