Advertisement

Inhibitory effect of corynoline isolated from the aerial parts ofcorydalis incisa on the acetylcholinesterase

  • Dae Keun Kim
Research Articles Article

Abstract

In the course of screening Korean natural products for acetylcholinesterase (AChE) inhibitory activity, it was found that a methanolic extract of the aerial parts ofCorydalis incisa (Papaver-aceae) showed significant inhibitory effects on AChE. Corynoline isolated from this plant inhibited AChE activity in a dose-dependent manner, and the IC50 value of corynoline was 30.6 μM. The AChE inhibitory activity of corynoline was reversible and noncompetitive.

Key words

Corydalis incisa Papaveraceae Acetylcholinesterase inhibitor Corynoline 

References

  1. Bartus, R. T., On neurodegenerative diseases, models, and treatment strategies: lessons learned and lessons forgotten a generation following the cholinergic hypothesis.Exp. Neurol., 163, 495–529 (2000).PubMedCrossRefGoogle Scholar
  2. Bartus, R. T., Dean, R. L., Beer, B., and Lippa, A. S., The cholinergic hypothesis of geriatric memory dysfunction.Science, 217, 408–414 (1982).PubMedCrossRefGoogle Scholar
  3. Broadwell, R. D., and Sofroniew, M. V., Serum proteins bypass the blood-brain fluid barriers for extracellular entry to the central nervous system.Exp. Neurol., 120, 245–263 (1993).PubMedCrossRefGoogle Scholar
  4. Chung, Y. K., Heo, H. J., Kim, E. K., Kim, H. K., Huh, T. L., Lim, Y., Kim, S. K., and Shin, D. H., Inhibitory effect of ursolic acid purified fro.Origanum majorana L. on the acetylcholinesterse.Mol. Cells, 11, 137–143 (2001).PubMedGoogle Scholar
  5. Ellman, G. L., Courtney, D., Valentino, A., and Featherstone, R. M., A new and rapid colorimetric determination of acetylcholinesterase activity.Biochem. Pharmacol., 7, 88–95 (1961).PubMedCrossRefGoogle Scholar
  6. Hwang, S. Y., Chang, Y. P., Byun, S. J., Jeon, M. H., and Kim, Y. C., An acetylcholinesterase inhibitor isolated from Cory-dalis Tuber and its mode of action.Kor. J. Pharmacogn., 27, 91–95 (1996).Google Scholar
  7. Kim, D. K., Eun, J. S., Shin, T. Y., Eom, D. O., and Lim, J. P., Benzo[c]phenanthridine alkaloids fromCorydalis incisa.Arch. Pharm. Res., 23, 589–591 (2000).PubMedCrossRefGoogle Scholar
  8. Park, C. H., Kim, S. H., Choi, W., Lee, Y. J., Kim, J. S., Kang, S. S., and Suh, Y. H., Novel anticholinesterase and antiamnesic activities of dehydroevodiamine, a constituent o.Evodia rutaecarpa.Planta Med., 62, 405–409 (1996).PubMedCrossRefGoogle Scholar
  9. Perry, E. K., The cholinergic hypothesis-ten years on.Br. Med. Bull., 42, 63–69 (1986).PubMedGoogle Scholar
  10. Rhee, I. K., van de Meent, M., Ingkaninan, K., and Verpoorte, R., Screening for acetylcholinesterase inhibitors from Am-aryllidaceae using silica gel thin-layer chromatography in combination with bioactivity staining.J. Chromatogr. A, 915, 217–223 (2001).PubMedCrossRefGoogle Scholar
  11. Riger, F., Shelanski, M. L., and Greene, L. A., The effects of nerve growh factor on acetylcholinesterase and its multiple forms in cultures of rat PC12 pheochromocytoma cells; increased total specific activity and appearance of the 16 S molecular form.Dev. Biol., 76, 238–243 (1980).CrossRefGoogle Scholar
  12. Selkoe, D. J., Alzheimer’s disease; a central role for amyloid.J. Neuropathol. Exp. Neurol., 53, 438–447 (1994).PubMedCrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2002

Authors and Affiliations

  1. 1.College of PharmacyWoosuk UniversitySamryeKorea

Personalised recommendations