Archives of Pharmacal Research

, Volume 25, Issue 5, pp 643–646

Phytol, SSADH inhibitory diterpenoid ofLactuca sativa

  • Myun-Ho Bang
  • Soo Young Choi
  • Tae-O Jang
  • Sang Kook Kim
  • Oh-Shin Kwon
  • Tae-Cheon Kang
  • MooHo Won
  • Jinseu Park
  • Nam-ln Baek
Research Articles Articles

DOI: 10.1007/BF02976937

Cite this article as:
Bang, MH., Choi, S.Y., Jang, TO. et al. Arch Pharm Res (2002) 25: 643. doi:10.1007/BF02976937

Abstract

The succinic semialdehyde dehydrogenase (SSADH) inhibitory component was isolated from the EtOAc fraction ofLactuca sativa through repeated column chromatography; then, it was identified as phytol, a diterpenoid, based on the interpretation of several spectral data. Incubation of SSADH with the phytol results in a time-dependent loss of enzymatic activity, suggesting that enzyme modification is irreversible. The inactivation followed pseudo-first-order kinetics with the second-rate order constant of 6.15 x 10-2 mM-1min-1. Complete protection from inactivation was afforded by the coenzyme NAD+, whereas substrate succinic semialdehyde failed to prevent the inactivation of the enzyme; therefore, it seems likely that phytol covalently binds at or near the active site of the enzyme. It is postulated that the phytol is able to elevate the neurotransmitter GABA levels in central nervous system through its inhibitory action on one of the GABA degradative enzymes, SSADH.

Key words

Lactuca sativa Anticonvulsant SSADH inhibition Phytol 

Copyright information

© The Pharmaceutical Society of Korea 2002

Authors and Affiliations

  • Myun-Ho Bang
    • 1
  • Soo Young Choi
    • 2
  • Tae-O Jang
    • 1
  • Sang Kook Kim
    • 3
  • Oh-Shin Kwon
    • 3
  • Tae-Cheon Kang
    • 4
  • MooHo Won
    • 4
  • Jinseu Park
    • 2
  • Nam-ln Baek
    • 1
  1. 1.Graduate School of Biotechnology & Plant Metabolism Research CenterKyunghee UniversitySuwonKorea
  2. 2.Department of Genetic Engineering, Division of Life SciencesHallym UniversityChunchonKorea
  3. 3.Department of BiochemistryKyungpook National UniversityTaeguKorea
  4. 4.Department of Anatomy, College of MedicineHallym UniversityChunchonKorea
  5. 5.Graduate School of BiotechnologyKyung Hee UniversityKiheung-Eup, Yongin-Si, Kyunggi-DoKorea

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