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Antitumor components ofAgrocybe cylindracea

  • Jin Won Hyun
  • Chae Kyun Kim
  • Seol Hee Park
  • Jong Myung Yoon
  • Mi Ja Shim
  • Chary Yuil Kang
  • Eung Chil Choi
  • Byong Kak Kim
Research Articles

Abstract

To find pharmacologically active components ofAgrocybe cylindracea, its basidiocarps were extracted with water. The extracts were separated by DEAE cellulose column chromatography, Sepharose CL-4B gel filtration, and Concanavalin A-Sepharose 4B affinity chromatography. Among the obtained fractions fromA. cylindracea, fraction IN which was the neutral proteinbound-polysaccharide fraction exhibited a marked antitumor activity and it was tentatively named “Cylindan”. It showed about 70% of tumor inhibition against the solid form of sarcoma 180 when a dose of 30 mg/kg/day was intraperitoneally injected into ICR mice. When each fraction was examined by chemical analysis, Cylindan consisted of 85% polysaccharide, 3% protein and 1% hexosamine. Its polysaccharide moiety contained glucose, mannose, fucose and galactose and its protein moiety contained the comparatively large amounts of aspartic acid and glycine, and other 11 amino acids.

Key words

Basidiomycete Agrocybe cylindracea Edible mushrooms Protein bound-polysaccharides Antitumor activity 

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Copyright information

© The Pharmaceutical Society of Korea 1996

Authors and Affiliations

  • Jin Won Hyun
    • 1
  • Chae Kyun Kim
    • 1
  • Seol Hee Park
    • 1
  • Jong Myung Yoon
    • 1
  • Mi Ja Shim
    • 2
  • Chary Yuil Kang
    • 1
  • Eung Chil Choi
    • 1
  • Byong Kak Kim
    • 1
  1. 1.College of PharmacySeoul National UniversitySeoulKorea
  2. 2.Seoul City UniversitySeoulKorea

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