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Archives of Pharmacal Research

, Volume 26, Issue 6, pp 458–462 | Cite as

Antioxidant activity from the stem bark ofAlbizzia julibrissin

  • Mee Jung Jung
  • Hae Young Chung
  • Sam Sik Kang
  • Jin Ho Choi
  • Kae Sun Bae
  • Jae Sue Choi
Article

Abstract

The antioxidant activity of the stem bark fromAlbizzia julibrissin was evaluated for its potential to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, to inhibit the generation of the hydroxyl radical (OH), total reactive oxygen species (ROS) and to scavenge authentic peroxynitrites (ONOO). The methanol extract ofA. julibrissin exhibited strong antioxidant activity in the tested model systems. Therefore, it was further fractionated using several solvents. The antioxidant activity of the individual fractions were in the order of ethyl acetate (EtOAc) <n-butanol (n-BuOH) < dichloromethane (CH2CI2) < and water (H2O). The ethyl acetate soluble fraction, which exhibited strong antioxidant activity, was further purified by repeated silicagel, Sephadex LH-20 and RP-18 gel column chromatography. Sulfuretin (1) and 3′,4′,7-trihydroxy-flavone (2) were isolated as the active principles. Compounds1 and2 exhibited good activity in all tested model systems. Compound1 exhibited five times more inhibitory activity on the total ROS than Trolox. Compound2 showed six times stronger DPPH radical scavenging activity than L-ascorbic acid. These results show the possible antioxidant activity of theA. julibrissin crude extract and its major constituents

Key words

Albizzia julibrissin Leguminosae Sulfuretin 3′,4′,7-Trihydroxyflavone Antioxidant 

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Copyright information

© The Pharmaceutical Society of Korea 2003

Authors and Affiliations

  • Mee Jung Jung
    • 1
  • Hae Young Chung
    • 2
  • Sam Sik Kang
    • 3
  • Jin Ho Choi
    • 1
  • Kae Sun Bae
    • 4
  • Jae Sue Choi
    • 1
  1. 1.Faculty of Food Science and BiotechnologyPukyong National UniversityBusanKorea
  2. 2.College of PharmacyPusan National UniversityBusanKorea
  3. 3.Natural Products Research InstituteSeoul National UniversitySeoulKorea
  4. 4.College of Natural Resources and Life ScienceDong-A UniversityBusanKorea

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