Archives of Pharmacal Research

, Volume 26, Issue 6, pp 458–462 | Cite as

Antioxidant activity from the stem bark ofAlbizzia julibrissin

  • Mee Jung Jung
  • Hae Young Chung
  • Sam Sik Kang
  • Jin Ho Choi
  • Kae Sun Bae
  • Jae Sue ChoiEmail author


The antioxidant activity of the stem bark fromAlbizzia julibrissin was evaluated for its potential to scavenge 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, to inhibit the generation of the hydroxyl radical (OH), total reactive oxygen species (ROS) and to scavenge authentic peroxynitrites (ONOO). The methanol extract ofA. julibrissin exhibited strong antioxidant activity in the tested model systems. Therefore, it was further fractionated using several solvents. The antioxidant activity of the individual fractions were in the order of ethyl acetate (EtOAc) <n-butanol (n-BuOH) < dichloromethane (CH2CI2) < and water (H2O). The ethyl acetate soluble fraction, which exhibited strong antioxidant activity, was further purified by repeated silicagel, Sephadex LH-20 and RP-18 gel column chromatography. Sulfuretin (1) and 3′,4′,7-trihydroxy-flavone (2) were isolated as the active principles. Compounds1 and2 exhibited good activity in all tested model systems. Compound1 exhibited five times more inhibitory activity on the total ROS than Trolox. Compound2 showed six times stronger DPPH radical scavenging activity than L-ascorbic acid. These results show the possible antioxidant activity of theA. julibrissin crude extract and its major constituents

Key words

Albizzia julibrissin Leguminosae Sulfuretin 3′,4′,7-Trihydroxyflavone Antioxidant 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Blois, M. S., Antioxidant determination by the use of a stable free radical.Nature, 181, 1199–1202 (1958).CrossRefGoogle Scholar
  2. Branen, A. L., Toxicology and biochemistry of butylated hydroxy-anisole and butylated hydroxytoluene.J. Am. Oil Chem.Soc., 52, 59–63 (1975).PubMedCrossRefGoogle Scholar
  3. Chamsuksai, P., Choi, J. S., and Woo, W. S., 3′,4′,7-Trihydroxy-flavone i.Albizzia julibrissin.Arch. Pharm. Res., 4, 129–131 (1981).CrossRefGoogle Scholar
  4. Choi, J. W., Yoon, B-. J., Huh, K., Park, K-. Y., Lee, K-. T., and Park, H-. J., Anti-rheumatoidal effect of sulfuretin isolated from the heartwood ofRhus veniciflua in rats and mice.Nutraceuticals and Food, 7, 347–352 (2002).Google Scholar
  5. Farag, S. F., EI-Emary, N. A., Makboul, M. A., Hasanean, H. A., and Niwa, M., Phytochemical investigation o.Wedelia prostata Hooket ARN. (HEMSL); -The ethyl acetate soluble fraction of the methanol extract.Bull. Pharm. Sci., 20, 37–46 (1997).Google Scholar
  6. Jung, M. J., Kang, S. S., and Choi, J. S., A new 4-hydoxy-dodec-2E-enedioic acid from the stem bark o.Albizzia julibrissin.Arch. Pharm. Res., 26, 207–209 (2003).CrossRefGoogle Scholar
  7. Kang, S. S. and Woo, W. S., Sapogenins fromAlbizzia julibrissin.Arch. Pharm. Res., 6, 25–28 (1983).CrossRefGoogle Scholar
  8. Kim, T. J.,Korean Resources Plants. II. Seoul National University Press, Korea, pp. 194–195 (1996).Google Scholar
  9. Kooy, N. W., Royall, J. A., Ischiropoulos, H., and Beckman, J. S., Peroxynitrite-mediated oxidation of dihydrorhodamine 123.Free Rad. Biol. Med., 16, 149–156 (1994).PubMedCrossRefGoogle Scholar
  10. Label, C. P. and Bondy, S. C., Sensitive and rapid quantitation of oxygen reactive species formation in rat synaptosomes.Neurochem. Int., 17, 435–441 (1990).CrossRefGoogle Scholar
  11. Lee, S. J., Son, K. H., Chang, H. W., Do, J. C., Jung, K. Y., and Kang, S. S., Antiinflammatory activity of naturally occurring flavone and flavonol glycosides.Arch. Pharm. Res., 16, 25–28 (1993).CrossRefGoogle Scholar
  12. Morel, I., Lescoat, G., Cognel, P., Sergent, O., Pasdelop, N., Brissot, P., Cillard, P., and Cillard, J., Antioxidant and iron-chelating activities of the flavonoids catechins, quercetin and diosmetin on iron-loaded rat hepatocyte cultures.Biochem. Pharmacol., 45, 13–19 (1993).PubMedCrossRefGoogle Scholar
  13. Oshima, H., Yoshie, Y., Auriol, S., and Gillbert, I., Antioxidant and pro-oxidant actions of flavonoids: Effects on DNA damage induced by nitric oxide, peroxynitrite and nitroxyl anion.Free Rad. Biol. Med., 25, 1057–1065 (1998).CrossRefGoogle Scholar
  14. Park, H-. J., Kwon, S-. H., Kim, G-. T., Lee, J-. H., Choi, J-. H., Choi, J. W., and Park, K-. Y., Physicochemical and biological characteristics of flavonoids to isolated from the heartwoods ofRhus vernicrflus. Kor.J. Pharmacogn., 31, 345–350 (2000).Google Scholar
  15. Podrez, E. A., Schmitt, D., Hoff, H. F., and Hazen, S. L., Myelo-peroxidase-generated reactive nitrogen species convert LDL into an atherogenic formin vitro.J. Clin. Invest., 103, 1547–1560 (1999).PubMedCrossRefGoogle Scholar
  16. Rice-Evans, C. A., Miller, N., and Paganga, G., Structure-anti-oxidant activity relationships of flavonoids and phenolic acids.Free Rad. Biol. Med., 20, 933–956 (1996).PubMedCrossRefGoogle Scholar
  17. Sagar, S., Kallo, I. J., Kaul, N., Ganguly, N. K., and Sharma, B., Oxygen free radicals in essential hypertension.Mol. Cell Biochem., 111, 103–108 (1992).PubMedCrossRefGoogle Scholar
  18. Salah, N., Miller, N. J., Paganga, G., Tijburg, L., Bolwell, G. P., and Rice-Evans, C., Polyphenols flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants.Arch. Biochem. Biophys., 322, 339–346 (1995).PubMedCrossRefGoogle Scholar
  19. Squadrito, G. L. and Pryor, W. A., Oxidative chemistry of nitric oxide: the roles of superoxide, peroxynitrite, and carbon dioxide.Free Rad. Biol. Med., 25, 392–403 (1998).PubMedCrossRefGoogle Scholar
  20. Westenburg, H. E., Lee, K-. J., Lee, S. K., Fong, H. H. S., and Van Breemen, R. B., Activity-guided isolation of antioxidative constituents ofCotinus coggygria.J. Nat. Prod., 63, 1696–1698 (2000).PubMedCrossRefGoogle Scholar
  21. Woo, W. S., and Kang, S. S., Isolation of a new monoterpene conjugated triterpenoid from the stem bark ofAlbizzia julibrissin.J. Nat. Prod., 47, 547–549 (1984).PubMedCrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2003

Authors and Affiliations

  • Mee Jung Jung
    • 1
  • Hae Young Chung
    • 2
  • Sam Sik Kang
    • 3
  • Jin Ho Choi
    • 1
  • Kae Sun Bae
    • 4
  • Jae Sue Choi
    • 1
    Email author
  1. 1.Faculty of Food Science and BiotechnologyPukyong National UniversityBusanKorea
  2. 2.College of PharmacyPusan National UniversityBusanKorea
  3. 3.Natural Products Research InstituteSeoul National UniversitySeoulKorea
  4. 4.College of Natural Resources and Life ScienceDong-A UniversityBusanKorea

Personalised recommendations