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Apoptosis induction of persicae semen extract in human promyelocytic leukemia (hl-60) cells

  • Kwon Hee-Young
  • Hong Seon-Pyo
  • Hahn Dong-Hoon
  • Kim Jeong Hee
Research Articles Articles

Abstract

The major ingredient of Persicae Semen is a cynogenic compound, amygdalin (D-mandelonitrile-β-gentiobioside). Controversial results on the anticancer activity of amygdalin were reported due to its conversion to its inactive isomer, neoamygdalin. In order to inhibit the epimerization of amygdalin, we used newly developed simple acid boiling method in preparation of Persicae Semen extract. HPLC analysis revealed most of amygdalin in Persicae Semen extract was active D-form. Persicae Semen extract was used to analyze its effect on cell proliferation and induction of apoptosis in human promyelocytic leukemia (HL-60) cells. Persicae Semen extract was cytotoxic to HL-60 cells with IC50 of 6.4 mg/mL in the presence of 250 nM of (β-glucosidase. The antiproliferative effects of Persicae Semen extract appear to be attributable to its induction of apoptotic cell death, as Persicae Semen extract induced nuclear morphology changes and intemucleosomal DNA fragmentation.

Key words

Persicae Semen Amygdalin Epimerization Apoptosis 

References

  1. Bertrand, R. and Sane, A. T., Caspase inhibition in camptothecintreated U-937 is coupled with a shift from apoptosis to transient G1 arrest followed by necrotic cell death.Cancer Res., 59, 3565–3569 (1999).PubMedGoogle Scholar
  2. Brimer, L., Cicalini, A. R., Federici, F., Nout, R. M., Petruccioli, M., and Pulci, V., Two-step hydrolysis of amygdalin in molds.Riv. Biol., 89, 493–496 (1996).PubMedGoogle Scholar
  3. Dicenta, F., Martinez-Gomez, P., Grane, N., Martin, M. L., Leon, A., Canovas, J. A., and Berenguer, V., Relationship between cyanogenic compounds in kernels, leave and roots of sweet and bitter kernelled almonds.J. Agric. Food Chem., 50, 2149–2152 (2002).PubMedCrossRefGoogle Scholar
  4. Hyun, S. J., Yoon, M. Y., Kim, T. H., Kim, and J. H., Enhancement of mitogen-stimulated proliferation of low dose radiationadapted mouse splenocytes.Anticancer Res., 17, 225–229 (1997).PubMedGoogle Scholar
  5. Hwang, E. Y., Lee, J. H., Lee, Y. M., and Hong, S. P., Reversephase HPLC separation of D-amygdalin and neoamygdalin optimum conditions for inhibition of racemization of amygdalin.Chem. Pharm. Bull., 50, 1373–1375 (2002).PubMedCrossRefGoogle Scholar
  6. Isozaki, T., Matano, Y., Yamamoto, K., and Kosaka, N., Quantitive determination of amygdain cpimers by cyclodextrin-modified micellar electrokinetic chromatography.J. Chrommatography A., 923, 249–254 (2001).CrossRefGoogle Scholar
  7. Kang, S. H., Jung, H., Kim, N., Shin, D. H., and Chung, D. S., Micellar electrokinetic chromatography for the analysis of Damygdalin its epimer in apricot kernel.J. Chromatogr A., 866, 253–259 (2000).PubMedCrossRefGoogle Scholar
  8. Kerr, J. R., Winterford, C. M., and Harmon, B. V., Apoptosis: Its significance in cancer and cancer therapy.Cancer, 73, 2013–2026 (1994).PubMedCrossRefGoogle Scholar
  9. Koeffler, H. P., Lowe, L., and Golde, D. W., Amygdalin (Laetrile): Effect on clonogenic cells from human myeloid leukemia cell lines and normal human marrow.Cancer Treat. Rep., 64, 105–109 (1980).PubMedGoogle Scholar
  10. Lee, S. J., Ko, W. G., Kim, J. H., Sung, G. H., Lee, S. J., Moon, C. K., and Lee, B. H., Induction of apoptosis by a novel intestinal metabolite of Ginseng Saponin via cytochrome cmediated activation of Caspase-3 protease.Biochem. Pharm., 60, 667–685 (2000).Google Scholar
  11. Lilenbaum, R. C. and Green, M. R., Novel chemotherapeutic agents in the treatment of non-small-cell lung cancer.J. Clin. Onco., 11, 1391–1402 (1993).Google Scholar
  12. Lim, T. H., Leem, M. J., Shin, D. H., Chang, H. B., Hong, S. W., Moon, E. Y., Lee, D. K., Yoon, S. J., and Woo, W. S., Cytotoxic constituents from the roots ofAnthriscus sylvestris.Arch. Pharm. Res., 22, 208–212 (1999).PubMedGoogle Scholar
  13. Llorens, O., Filizola, M., Spisani, S., Marastoni, M., Herranz, C., and Perez, J. J., Amygdain binds to the CD4 receptor as suggested from molecular modeling studies.Bioorg. Med. Chem. Lett., 8, 781–786 (1998).PubMedCrossRefGoogle Scholar
  14. Moertel, C. G., Pleming, T. R., Rubin, J., Kvols, L. K., Sarna, G., Koch, R., Currie, V. E., Yo, C. W., Jones, S. E., and Davignon, J. P.N. Engl. Med., 306, 201–206 (1982).Google Scholar
  15. Monks, A., Scudiero, D., Skehan, P., Shoemaker, R., Paull, K., Vistica, D., Hose, C., Langley, J., Cronise, P., Vaigro-Wolff, A., Gray-Goodrich, M., Campbell, H., Mayo, J., and Boyd, M., Feasibility of a high-flux anticancer drug screening using a diverse panel of human tumor cell lines.J. Natl. Cancer Inst., 83, 757–766 (1991).PubMedCrossRefGoogle Scholar
  16. Newmark, J., Brady, R. O., Grimley, P. M., Gal, A. E., Waller, S. G., and Thistlethwaite, J. R., Amygdalin (Laetrile) and prunasin beta-glucosidases: Distribute in germ free rat and human tumor tissue.Proc. Natl. Acad. Sci. USA, 78, 6513–6516 (1981).PubMedCrossRefGoogle Scholar
  17. Pezzuto, J. M., Plant-derived anticancer agents.Biochem Pharm., 53, 121–133 (1997).PubMedCrossRefGoogle Scholar
  18. Strugala, G. J., Stahl, R., Elsenhans, B., Rauws, A. G., and Forth, W., Small-intestinal transfer mechanism of prunasin, the primary metabolite of the cyanogenic glycoside amygdain.Hum Exp Toxicol, 14, 895–901 (1995).PubMedCrossRefGoogle Scholar
  19. Syrigos, K. N., Rowlinson-Busza, G., and Epenetos, A. A.,In vitro cytotoxicity following specific activation of amygdain beta-glucosidase conjugated to a bladder cancer-associated monoclonal antibody.Int. J. Cancer, 78, 712–179 (1998).PubMedCrossRefGoogle Scholar
  20. Takayama, Y. and Kawai, S., Study on the prevention of racemization of amygdain.Chem. Pharm. Bull., 32, 772–781 (1984).Google Scholar
  21. Yuan, D., Komatsu, K., Cui, Z., and Kano, Y., Pharmacological properties of traditional medicines. XXV. Effect of ephedrine, amygdalin, glycyrrhizin, gypsum and their combinations on body temperature and body fluid.Biol. Pharm. Bull., 22,165–171 (1999).PubMedGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2003

Authors and Affiliations

  • Kwon Hee-Young
    • 1
  • Hong Seon-Pyo
    • 2
  • Hahn Dong-Hoon
    • 1
  • Kim Jeong Hee
    • 1
  1. 1.Department of BiochemistryCollege of DentistrySeoulKorea
  2. 2.Department of Oriental Pharmaceutical SciencesKyung Hee UniversitySeoulKorea

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