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Costunolide induces differentiation of human leukemia HL-60 cells

  • Jung-Hye Choi
  • Bo-Rim Seo
  • Seong-Hoon Seo
  • Kyung-Tae LeeEmail author
  • Jae-Hoon Park
  • Hee-Juhn Park
  • Jong-Won Choi
  • Yoshie Itoh
  • Ken-ichi Miyamoto
Research Articles Articles

Abstract

Costunolide has been reported to be a cytotoxic and chemopreventive agent. This work investigated the mechanism of the antiproliferative effect of costunolide and determined that it induced differentiation of the human leukemia cell line HL-60. Costunolide exhibited a potent antiproliferative activity against HL-60 cells. It was also found to be a potent inducer of differentiation in human leukemia derived HL-60 cells through the examination of differentiation markers, as assessed by the reduction of nitroblue tetrazolium, the increase in esterase activities and phagocytic activity, morphology change and the expression of CD14 and CD66b surface antigens. These results, accompanied by a decline in the expression of c-myc protein, suggest that costunolide induces differentiation of human leukemia cells to granulocytes and monocytes/macrophages lineage.

Key words

Costunolide Differentiation Granulocyte Monocyte HL-60 cell 

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Copyright information

© The Pharmaceutical Society of Korea 2002

Authors and Affiliations

  • Jung-Hye Choi
    • 1
  • Bo-Rim Seo
    • 1
  • Seong-Hoon Seo
    • 1
  • Kyung-Tae Lee
    • 1
    Email author
  • Jae-Hoon Park
    • 2
  • Hee-Juhn Park
    • 3
  • Jong-Won Choi
    • 4
  • Yoshie Itoh
    • 5
  • Ken-ichi Miyamoto
    • 5
  1. 1.College of PharmacyKyung Hee UniversitySeoul
  2. 2.College of MedicineKyung Hee UniversitySeoul
  3. 3.Division of Applied Plant SciencesSangji UniversityWonju
  4. 4.College of PharmacyKyung-Sung UniversityPusanKorea
  5. 5.Department of Hospital Pharmacy, School of MedicineKanazawa UniversityKanazawaJapan

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