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Archives of Pharmacal Research

, Volume 22, Issue 2, pp 219–224 | Cite as

Isolation and identification of succinic semialdehyde dehydrogenase inhibitory compound from the rhizome ofGastrodia elata blume

  • Nam-In Baek
  • Soo Young Choi
  • Jin Kyu Park
  • Sung-Woo Cho
  • Eun-Mi Ahn
  • Seong Gyu Jeon
  • Byung Ryong Lee
  • Jae Hoon Bahn
  • Yong Kyu Kim
  • Il Hwan Shon
Research Articles Natural Products

Abstract

In our search for the anticonvulsant constituent ofGastrodia elata repeated column chromatographies guided by activity assay led to isolation of an active compound, which was identified as gastrodin on the basis of spectral data. Brain succinic semialdehyde dehydrogenase (SSADH) was inactivated by preincubation with gastrodin in a time-dependent manner and the reaction was monitored by absorption and fluorescence spectroscopic methods. The inactivation followed pseudo-first-order kinetics with the second-rate order constant of 1.2×103 M−1 min−1. The time course of the reaction was significantly affected by the coenzyme NAD+, which affected complete protection against the loss of the catalytic activity, whereas substrate succinic semialdehyde failed to prevent the inactivation of the enzyme. It is postulated that the gastrodin is able to elevate the neurotransmitter GABA levels in central nervous system by inhibitory action on one of the GABA degradative enzymes, SSADH.

Key words

Gastrodia elata Anticonvulsant Gastrodin Brain GABA shunt Succinic semialdehyde dehydrogenase 

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Copyright information

© The Pharmaceutical Society of Korea 1999

Authors and Affiliations

  • Nam-In Baek
    • 5
    • 6
  • Soo Young Choi
    • 1
  • Jin Kyu Park
    • 2
  • Sung-Woo Cho
    • 3
  • Eun-Mi Ahn
    • 5
  • Seong Gyu Jeon
    • 1
  • Byung Ryong Lee
    • 1
  • Jae Hoon Bahn
    • 1
  • Yong Kyu Kim
    • 2
  • Il Hwan Shon
    • 4
  1. 1.Department of Genetic Engineering, Division of Life SciencesHallym UniversityChunchonKorea
  2. 2.Korea Ginseng & Tobacco Research InstituteTaejeonKorea
  3. 3.Department of Biochemistry, College of MedicineUniversity of UlsanSeoulKorea
  4. 4.Daeduk R & D CenterDaelim Industrial Co.TaejeonKorea
  5. 5.Department of Life Sciences & ResourcesKyung Hee UniversityYongin-Si, Kyunggi-DoKorea
  6. 6.Institute of Life Science & ResourcesKyung Hee UniversityYongin-Si, Kyunggi-DoKorea

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