Effect of ginseng saponin on gap junction channel reconstituted with connexin32

  • Eun Jung Hong
  • Keun Huh
  • Seung Keun Rhee
Research Articles
Received:

Abstract

Panax-ginseng saponin has been known to exert various pharmacological effects on cellular metabolism. This study was performed to determine the effect of ginseng saponin on gap junction channel-mediated intercellular communication, using an establishedin vitro system of reconstituted gap junction channels. Gap junction channels are a specialized plasma membrane fraction, which are permeable to relatively large water-soluble molecules. The sucrose permeable property of reconstituted gap junction channels was completely inhibited with 0.1% (w/v) of ginseng saponin. We also compared the effect of ginseng saponin with that of Triton X-100, a nonionic detergent, on the same system. Triton X-100 showed significantly different effect on sucrose-permeability of gap junction channel from that was affected by ginseng saponin. The structures of liposomes containing gap junction channels was significantly destroyed by Triton X-100.

Key words

Ginseng Gap junction channel Connexin32 Sucrose-permeability Triton X-100 Reconstitution 
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Copyright information

© The Pharmaceutical Society of Korea 1996

Authors and Affiliations

  • Eun Jung Hong
    • 1
  • Keun Huh
    • 2
  • Seung Keun Rhee
    • 1
  1. 1.Department of BiochemistryYeungnam UniversityKyongsanKorea
  2. 2.Department of PharmacologyYeungnam UniversityKyongsanKorea

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