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Archives of Pharmacal Research

, Volume 24, Issue 5, pp 407–411 | Cite as

Quantitative determination of eleutheroside B and E fromAcanthopanax species by high performance liquid chromatography

  • Jong Seong Kang
  • Pham Tuan Linh
  • Xing Fu Cai
  • Hang Sup Kim
  • Jung Joon Lee
  • Young Ho Kim
Research Article Medicinal Chemistry & Natural Products

Abstract

Reversed-phase high performance liquid chromatographic method was applied for the determination of eleutheroside B and E in the variousAcanthopanax species collected in Korea. The stationary phase used was Zorbax 300 SB C18 and a mobile phase program was used, which started at 6% acetonitrile for 2 min, and then a linear gradient was operated for the next 18 min to 17% acetonitrile at a flow rate of 1.0 ml/min. The column effluent was monitored at UV 210 nm. Identification was carried out by comparing the retention time and the LC/MS spectrum of each peak corresponding to eleutheroside B and E from sample with those of standards. In general, the contents of eleutheroside B and E in stems were higher than those in roots.Acanthopanax species could be classified into two groups based upon the contents of eleutheroside B and E: one group contains no or very little eleutheroside B and another contains both eleutheroside B and E.

Key words

Acanthopanax species Eleutheroside B Eleutheroside E Gradient elution HPLC 

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Copyright information

© The Pharmaceutical Society of Korea 2001

Authors and Affiliations

  • Jong Seong Kang
    • 1
  • Pham Tuan Linh
    • 1
  • Xing Fu Cai
    • 1
  • Hang Sup Kim
    • 2
    • 1
  • Jung Joon Lee
    • 2
    • 1
  • Young Ho Kim
    • 1
  1. 1.College of PharmacyChungnam National UniversityTaejonKorea
  2. 2.Korea Research Institute of Bioscience and BiotechnologyTaejonKorea

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