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Archives of Pharmacal Research

, Volume 29, Issue 10, pp 874–878 | Cite as

Effects of methyl gallate on arachidonic acid metabolizing enzymes: Cyclooxygenase-2 and 5-lipoxygenase in mouse bone marrow-derived mast cells

  • Se Jong Kim
  • Meihua Jin
  • Eunkyung Lee
  • Tae Chul Moon
  • Zhejiu Quan
  • Ju Hye Yang
  • Kun Ho Son
  • Kil-Ung Kim
  • Jong Kun Son
  • Hyeun Wook Chang
Articles Drug Development

Abstract

Methyl gallate (MG) is a medicinal herbal product that is isolated fromPaeonia lactiflora that inhibits cyclooxygenase-2 (COX-2) dependent phases of prostaglandin D2 (PGD2) generation in bone marrow-derived mast cells (BMMC) in a concentration-dependent manner with an IC50 values of 17.0 μM. This compound also found inhibited the COX-2-dependent conversion of the exogenous arachidonic acid to PGD2 in a dose-dependent manner with an IC50 values of 19.0 μM, using a COX enzyme assay kit. However, at concentrations up to 80 μM, MG did not inhibit COX-2 protein expression in BMMC, indicating that MG inhibits COX-2 activity directly. Furthermore, MG consistently inhibited the production of leukotriene C4 (LTC4) in a dose dependent manner, with an IC50 value of 5.3 μM. These results demonstrate that MG has a dual cyclooxygenase-2/5-lipoxygenase inhibitory activity, which provide the basis for novel anti-inflammatory drugs.

Key words

Paeonia lactiflora Methyl gallate Inflammation Cyclooxygenase-2 5-Lipoxygenase Bone marrow-derived mast cells 

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Copyright information

© The Pharmaceutical Society of Korea 2006

Authors and Affiliations

  • Se Jong Kim
    • 1
  • Meihua Jin
    • 2
    • 1
  • Eunkyung Lee
    • 2
  • Tae Chul Moon
    • 2
  • Zhejiu Quan
    • 2
  • Ju Hye Yang
    • 2
  • Kun Ho Son
    • 3
  • Kil-Ung Kim
    • 4
  • Jong Kun Son
    • 2
  • Hyeun Wook Chang
    • 2
  1. 1.Research Institute of Natural Product Kyongbuk Provincial A. T. AUisungKorea
  2. 2.College of PharmacyYeungnam UniversityGyongsanKorea
  3. 3.Department of Food and NutritionAndong National UniversityAndongKorea
  4. 4.College of Agriculture and Life ScienceKyungpook National UniversityDaeguKorea

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