Protection of peroxynitrite-induced DNA damage by dietary antioxidants
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The present study was undertaken to test the hypothesis that dietary antioxidants protect DNA damage induced by peroxynitrite, a potent physiological inorganic toxin. The present study showed that dietary antioxidants such as (−)-epigallocatechin, gallate, quercerin, rutin, resveratrol, and ursolic acid inhibit single strand breaks in supercoiled plasmid DNA induced by 3-morpholinosyndromine N-ethylcarbamide (SIN-1), a generator of peroxynitrite, through the reaction between nitric oxide and superoxide anion. The formation of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) in calf thymus DNA by SIN-1 was also inhibited by dietary antioxidants. When U937 cells were incubated with 1 mM SIN-1 bolus, a significant increase of 8-OH-dG level was observed. However, oxidative DNA damage was significantly lower in the cells pre-treated with dietary antioxidants when cells were exposed to SIN-1.
Key wordsDietary antioxidants Peroxynitrite DNA damage
- Juedes, M. J. and Wogan, G. N., Peroxynitrite-induced mutation spectra of pSP189 following replication in bacteria and in human cells.Mut. Res., 349, 51–61 (1996).Google Scholar
- Park, J.-W. and Floyd R. A., Generation of strand breaks and formation of 8-hydroxy-2′-deoxyguanosine in DNA by a thiol/Fe3+/O2-catalyzed oxidation system.Arch. Biochem. Biophys., 12, 245–250 (1992).Google Scholar