Protection of peroxynitrite-induced DNA damage by dietary antioxidants

  • Hye Kyung Moon
  • Eun Sun Yang
  • Jeen Woo ParkEmail author
Articles Drug Development


The present study was undertaken to test the hypothesis that dietary antioxidants protect DNA damage induced by peroxynitrite, a potent physiological inorganic toxin. The present study showed that dietary antioxidants such as (−)-epigallocatechin, gallate, quercerin, rutin, resveratrol, and ursolic acid inhibit single strand breaks in supercoiled plasmid DNA induced by 3-morpholinosyndromine N-ethylcarbamide (SIN-1), a generator of peroxynitrite, through the reaction between nitric oxide and superoxide anion. The formation of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) in calf thymus DNA by SIN-1 was also inhibited by dietary antioxidants. When U937 cells were incubated with 1 mM SIN-1 bolus, a significant increase of 8-OH-dG level was observed. However, oxidative DNA damage was significantly lower in the cells pre-treated with dietary antioxidants when cells were exposed to SIN-1.

Key words

Dietary antioxidants Peroxynitrite DNA damage 


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Copyright information

© The Pharmaceutical Society of Korea 2006

Authors and Affiliations

  • Hye Kyung Moon
    • 1
  • Eun Sun Yang
    • 1
  • Jeen Woo Park
    • 1
    Email author
  1. 1.School of Life Sciences and Biotechnology and Agro-Biotechnology Education CenterKyungpook National UniversityTaeguKorea

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