Breast Cancer

, Volume 7, Issue 3, pp 221–230 | Cite as

Cytokines facilitate chemotactic motility of breast carcinoma cells

  • Koji Arihiro
  • Hiroyo Oda
  • Mayumi Kaneko
  • Kouki Inai
Original Article



Both growth and motility of various tumor cells have been shown to be influenced by surrounding cells such as lymphocytes, histiocytes and fibroblasts through various cytokines, growth factors and extracellular matrices. The role of cytokines and extracellular matrices produced by lymphocytes, histiocytes and fibroblasts on migration and invasion of breast carcinoma cells has not been fully investigated.


We investigated the effect of hepatocyte growth factor (HGF), interleukin (IL)-6, IL-8, IL-11, soluble type IV collagen and soluble laminin on the migration of 3 human breast carcinoma cell lines, MDA-MB-231, MCF-7 and T-47D, using a cell culture insert and a biocoat matrigel invasion chamber to assess migration across a matrigel-coated polyethylene telephtalate membrane.


HGF, IL-6, IL-11 and IL-8 induced significant migration of MDA-MB-231 cells depending on the dose of each cytokine. However, type IV collagen and laminin inhibited migration of MDA-MB-231 cells. In contrast, IL-8 inhibited migration of MCF-7 cells and IL-6 induced significant migration of T-47D cells, while no other cytokine or extracellular matrix induced significant migration of MCF-7 and T-47D cells. Only HGF induced significant invasion of MDA-MB-231 cells depending on the dose. MCF-7 and T-47D cells did not invade in response to any of the cytokines and extracellular matrices tested.


These results suggest the possibility that the potency of chemotaxis or chemoinvasion differs according to the breast carcinoma cell line and that various cytokines and extracellular matrices secreted by lymphocytes, histiocytes and fibroblasts in the stroma of breast carcinoma can affect the invasion of breast carcinoma cells.

Key words

Breast carcinoma cells Motility HGF IL-6 IL-11 



Hepatocyte growth factor




Estrogen receptor


Insulin-like growth factor receptors


Epidermal growth factor receptor


Transforming growth factor-beta receptor


Tumor necrosis factor receptor


Epidermal growth factor


Transforming growth factor


Basic fibroblast growth factor


Platelet-derived growth factor


Regulated on activation, normal T-cells, expressed and secreted


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Copyright information

© The Japanese Breast Cancer Society 2000

Authors and Affiliations

  • Koji Arihiro
    • 1
  • Hiroyo Oda
    • 1
  • Mayumi Kaneko
    • 1
  • Kouki Inai
    • 1
  1. 1.Second Department of PathologyHiroshima University School of MedicineJapan

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