The influence of the mycotoxin deoxynivalenol on jejunal glucose transport in pigs
Article
- 92 Downloads
- 5 Citations
Abstract
The experiments were aimed at evaluating whether the activity of the glucose transporter in the pig’s jejunum is affected by deoxynivalenol (DON)-contaminated feed. This is based on the ability of DON to inhibit the protein synthesis on cellular level which might also involve the synthesis of the intestinal glucose transporter.
Two groups of six castrated male pigs were restrictively fed a control or a DON contaminated diet (=5.7 mg/kg). Immediately after slaughtering the pigs, the mid-jejunum was quickly dissected, washed, frozen and stored at −80°C until preparations. Brush border membrane vesicles (BBMV) were prepared by Mg2+-EGTA-precipitation and differential centrifugation. The activity of the glucose transporter was examined by measuring the uptake of [3H]-glucose into the BBMV. The glucose uptake was quantified by using the rapid filtration technique.
The glucose uptake into BBMV followed Michaelis-Menten-kinetic. Comparison of the kinetic parameters Vmax (transport capacity) and Km (transport affinity) of the two treatment-groups showed no significant differences between feeding a DON-contaminated or control diet. It was concluded that dietary DON exposure (=5.7 mg/kg) for five weeks had no obvious influence on the intestinal glucose transporter and its activity in pigs.
Keywords
deoxynivalenol glucose transporter jejunum kinetic parameters pigPreview
Unable to display preview. Download preview PDF.
References
- 1.D’Mello JPF, MacDonald AMC (1997) Mycotoxins. Anim Feed Sci Tech 69: 155–166CrossRefGoogle Scholar
- 2.Rotter BA, Prelusky DB, Pestka JJ (1996) Toxicology of deoxynivalenol (vomitoxin). J Toxicol Environ Health 48 (1): 1–34PubMedCrossRefGoogle Scholar
- 3.Scientific Committee on Food (1999) Opinion of the Scientific Committee on Food onFusarium toxins, part 1: Deoxynivalenol (DON), European Commission, Health & Consumer Protection Directorate- General, Brussels, BelgiumGoogle Scholar
- 4.Young LG, McGirr L, Valli VE, Lumbsen JH, Lun A (1983) Vomitoxin in corn fed to young pigs. J. Anim Sci 57: 655–664PubMedGoogle Scholar
- 5.Harvey RB, Kubena LF, Huff WE, Corrier DE, Clark DE, Phillips TD (1989) Effects of aflatoxin, deoxynivalenol, and their combinations in the diets of growing pigs. Am J Vet Res 50 (4): 602–607PubMedGoogle Scholar
- 6.Feinberg B, McLaughlin CS (1989) Biochemical mechanism of action of trichothecene mycotoxins. In: Beasley VR (ed) Trichothecene mycotoxicosis: Pathophysiological effects. Vol 1, CRC Press, Inc. Boca Raton, Florida, USA: 27–35Google Scholar
- 7.Dänicke S (2003) Fusariotoxikosen. In: Wiesner E (ed) Handlexikon der tierärztlichen Praxis. Enke Verlag Stuttgart, Lieferung 213, 277 ik-1-28Google Scholar
- 8.Alvarado F (1966) Transport of sugars and amino acids in the intestine: evidence for a common carrier. Sci 151: 1010–1013CrossRefGoogle Scholar
- 9.Abel HJ, Flachowsky G, Jeroch H, Molnar S (1995) Nutztierernährung. Gustav Fischer Verlag, Jena StuttgartGoogle Scholar
- 10.Hunder G, Schümann K, Strugala G, Gropp J, Fichtl B, Forth W (1991) Influence of subchronic exposure to low dietary deoxynivalenol, a trichothecene mycotoxin, on intestinal absorption of nutrients in mice. Food Chem Toxicol 29 (12): 809–814PubMedCrossRefGoogle Scholar
- 11.Maresca M, Mahfoud R, Garmy N, Fantini J (2002) The mycotoxin deoxynivalenol affects nutrient absorption in human intestinal epithelial cells. J Nutr 132 (9): 2723–2731PubMedGoogle Scholar
- 12.Awad WA, Böhm J, Razzari-Fazeli E, Hulan HW, Zentek J (2004) Effects of deoxynivalenol on general performance and electrophysiological properties of intestinal mucosa of broiler chickens. Poult Sci 83: 1964–1972PubMedGoogle Scholar
- 13.Gesellschaft für Ernährungsphysiologie (GfE) (1987) Energie- und Nährstoffbedarf Landwirtschaftlicher Nutztiere, Nr. 4 Schweine. Ausschuss für Bedarsnormen, DLG-Verlag, Frankfurt (Main), GermanyGoogle Scholar
- 14.Goyarts T, Dänicke S, Rothkötter HJ, Spilke J, Tiemann U, Schollenberger M (2005) On the effects of a chronic deoxynivalenol intoxication on performance, haemaotological and serum parameters of pigs when diets are offered either forad libitum consumption or fed restrictively. J Vet Med A 52: 305–314CrossRefGoogle Scholar
- 15.Biber J, Stieger B, Haase W, Murer H (1981) A high yield preparation for rat kidney brush border membranes, different behavior of lysosomal markers. Biochim. Biophys Acta 647: 169–176PubMedCrossRefGoogle Scholar
- 16.Binder HJ, Murer H (1986) Potassium proton exchange in brush border membrane of rat ileum. J Memb Biol 91: 77–84CrossRefGoogle Scholar
- 17.Kaune R, Kassianoff I, Schröder B, Harmeyer J (1992) The effect of 1,25-dihydroxyvitamin D-3 deficiency on Ca2+-uptake into brush border membrane vesicles from pig small intestine. Biochim Biophys Acta 1109: 187–194PubMedCrossRefGoogle Scholar
- 18.Schröder B, Breves G (1996) Mechanism of phosphate uptake into brush- border membrane vesicles from goat jejunum. J Comp Physiol B 166: 230–240PubMedCrossRefGoogle Scholar
- 19.Ullrich KJ, Frömter E, Murer H (1979) Prinzipien des epithelialen Transports in Niere und Darm. Klin Wochenschr 57: 977–991PubMedCrossRefGoogle Scholar
- 20.Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein- dye binding. Anal Biochem 72: 248–254PubMedCrossRefGoogle Scholar
- 21.Peter R (1982) Klinische Chemie. Karger Verlag, Basel, MünchenGoogle Scholar
- 22.Mircheff AK, Wright EM (1976) Analytical isolation of plasma membranes of intestinal cells: identification of Na, K- ATPase rich membranes and the distribution of enzyme activities. J Membranes Biol 28: 309–333CrossRefGoogle Scholar
- 23.Graph PadTM Software: Graph Pad Prism Version 4.00 for Windows. San Diego, California, USA, www.graphpad.comGoogle Scholar
- 24.Ahearn GA, Murer H (1984) Functional roles of Na+ and H+ in SO42− — transport by rabbit ileal brush border membrane vesicles. J Memb Biol 78: 177–186CrossRefGoogle Scholar
- 25.Breves G, Walter C, Burmester M, Schröder B (2000)In vitro studies in the effects ofSaccharomyces boullardii andBacillus cereus var.toyoi on nutrient transport in pig jejunum. J Anim Physiol a Anim Nutr 84: 9–20CrossRefGoogle Scholar
- 26.Hunder G, Schümann K, Strugala G, Gropp J, Fichtl B, Forth W (1991) Influence of subchronic exposure to low dietary deoxynivalenol, a trichothecene mycotoxin, on intestinal absorption of nutrients in mice. Food Chem Toxicol 29 (12): 809–814PubMedCrossRefGoogle Scholar
- 27.Iverson F, Armstrong C, Nera E, Truelove E, Fernie S, Scott P, Stapley R, Hayward S, Gunner S (1995) Chronic feeding study of deoxynivalenol in B6C3F1 male and female mice. Teratog Carcinog Mutagen 15 (6): 283–306PubMedCrossRefGoogle Scholar
- 28.Dänicke S, Überschär KH, Valente H, Matthes S, Matthäus K, Halle I (2004) Effects of graded levels of,Fusarium-toxin-contaminated wheat in pekin duck diets on performance, health and metabolism of deoxynivalenol and zearalenon. Br Poult Sci 45 (2): 264–272PubMedCrossRefGoogle Scholar
- 29.Prelusky DB, Gerdes RG, Underholl KL, Rotter BA, Jui PY, Trenholm HL (1994) Effects of low-level dietary deoxynivalenol on haematogogical and clinical parameters of the pig. Nat Toxins 2 (3): 97–104PubMedCrossRefGoogle Scholar
Copyright information
© Society of Mycotoxin Research and Springer 2005