Abstract
The present experiment was undertaken to optimize the rooting responses in micropropagated shoots of sugarcane varieties CoS 96268 and CoS 95255. The results showed that half strength MS medium supplemented with NAA (5.0 mg/1) alongwith sucrose (50 g/1) proved to be the best combination for rooting in both the varieties. On this medium, maximum 75.4 ±7.1 % rooting in CoS 96268 and 81.6 ±7.6 % in CoS 95255 were recorded. Morphological observation of rooted shoots revealed that at higher concentration of NAA (7.0 mg/1) and sucrose (70 g/1) shoots became stunted, bore curved leaves and the roots became thin and brownish in colour whereas on medium containing NAA (5.0 mg/l) + sucrose (50 g/l) most of the roots appeared healthy, white with normal root hair.
References
Gosal, S.S., Thind, K.S. andDhaliwal, H.S. (1998). Micropropagation of sugarcane- An efficient protocol for commercial plant production.Crop Improv.,25 (2): 167–170.
Jadhav, A.B., Vaida, E.R., Aher, V.B. andPawar, A.M. (2001).In vitro multiplication of ‘Co86032’ sugarcane (Saccharum officinarum) hybrid.Indian Jour. of Agric. Sci. 71(2): 113–115.
Lee, T.S.G. (1986). Multiplication of sugarcane by apex culture.Turrialba.36: 231–236.
Lee, T.S.G. (1987). Micropropagation of sugarcane (Saccharum spp.).Plant Cell Tissue and Organ Culture.10: 47–55.
Liu, M.C. (1981).In vitro methods applied to sugarcane improvement In:Plant Tissue Culture (Ed. T.A. Thorpe) P. 299–323,Acad. Press, New York.
Nadar, H.M. andHeinz, D.J. (1977). Root and shoot development from sugarcane callus tissue.Crop. Sci.,17: 814–816.
Pawar, S.V., Patil, S.C., Jambhale, V.M., Naik, R.M. and Mehetre, S.S. (2002). Rapid multiplication of commercial sugarcane varieties through tissue culture.Indian Sugar, June, 2002, pp. 183–186.
Ramanand (2006).In vitro morphogenetic studies on crop improvement and multiplication in sugarcane (Saccharum Species Complex). Ph. D. Thesis, M.J.P. Rohilkhand University, Bareilly.
Ramanand andLal, M. (2003). An efficient protocol forin vitro micropropagation of sugarcane.Sugar Tech,6(1&2): 85–87.
Singh, B., Yadav, G.C. andLal, M. (2001). An efficient protocol for micropropagation of sugarcane using shoot tip expiant.Sugar Tech. 3(3): 113–116.
Sood, N., Srivastava, R. andGosal, S.S. (1999).In vitro multiplication and field evaluation of sugarcane.Proc. S.T.A.I.,61: 3–9.
Sreenivasan, T.V. andSreenivasan, J. (1992). Micropropagation of sugarcane varieties for increasing cane yield.SISSTA, Sugar J. 14: 61–64.
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Ramanand, Lal, M., Singh, S.B. et al. Optimization of rooting in micropropagated shoots of sugarcane. Sugar Tech 9, 95–97 (2007). https://doi.org/10.1007/BF02956920
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DOI: https://doi.org/10.1007/BF02956920