Mycotoxin Research

, Volume 21, Issue 1, pp 61–64 | Cite as

Molecular diagnosis of ochratoxinogenicAspergillus species

Article

Abstract

Toxigenic and non-toxigenic black aspergilli belonging to theAspergillus niger aggregate and toA. carbonarius were compared to each other and to strains of other species by DNA fingerprinting. AFLPs showed a clear separation ofA. niger andA. carbonarius. However, no clear correlation between the genetic similarity of the strains and the ability to produce ochratoxin A (OTA) was detected. Based on AFLP, marker sequences were chosen for the construction of SCAR-PCR primers for the detection ofA. carbonarius. A similar approach was used forA. ochraceus, another fungus of concern regarding ochratoxin A contamination of coffee. Cluster analysis ofA. ochraceus isolates mainly from Brazilian coffee showed a very close genetic similarity. Three species specific primer pairs were developed and one of these was used for the PCR and realtime PCR (RT-PCR) based detection of the mould in green coffee.A. ochraceus was specifically and rapidly detected and quantified in green coffee. A positive correlation between the amount of DNA and OTA content was established.

Keywords

Aspergillus ochratoxin A green coffee PCR 
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Copyright information

© Society of Mycotoxin Research and Springer 2005

Authors and Affiliations

  1. 1.Lehrstuhl für Technische MikrobiologieTU MünchenFreising

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