Mycotoxin Research

, Volume 22, Issue 1, pp 27–32 | Cite as

Breeding for resistance to aflatoxin accumulation in maize

Article

Abstract

Contamination of maize,Zea mays, grain with aflatoxin, a naturally occurring toxin produced byAspergillus flavus, frequently reduces the value and marketability of maize produced in the southern USA. Drought, high temperatures, and insect damage are often associated with high levels of maize aflatoxin contamination. Growing resistant maize hybrids is generally considered the most feasible method of reducing or eliminatingA. flavus infection and subsequent accumulation of aflatoxin. Developing appropriate screening techniques and identifying maize germplasm with resistance to aflatoxin contamination provides the foundation for a breeding program. Only a few sources of aflatoxin resistance have been identified. Four germplasm lines (Mp313E, Mp420, Mp715, and Mp717) have been developed and released by USDA-ARS at Mississippi State University. NC 388, developed at North Carolina State University, is reported as another putative source of aflatoxin resistance. Conventional phenotypic selection was used to successfully combine resistance to aflatoxin contamination from two of these lines, Mp313E and Mp715, with desirable agronomic qualities from Va35. The identification of quantitative trait loci (QTL) associated with resistance to aflatoxin contamination will also permit the use of marker assisted selection in transferring resistance into elite germplasm lines. Development of parental inbreds that combine aflatoxin resistance with superior agronomic quality is an essential component of a hybrid maize breeding program designed to reduce or eliminate aflatoxin contamination.

Keywords

aflatoxin Aspergillus flavus maize mycotoxin plant breeding 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Castegnaro M, McGregor D (1998) Carcinogenic risk assessment of mycotoxins. Rev Med Vet 149: 671–678Google Scholar
  2. 2.
    Payne G (1992) Aflatoxins in maize. Crit Rev Plant Sci 10: 423–440CrossRefGoogle Scholar
  3. 3.
    Widstrom M (1996) The aflatoxin problem with corn grain. Adv Agron 56: 219–280CrossRefGoogle Scholar
  4. 4.
    McMillian W, Wilson D, Widstrom N (1985) Aflatoxin contamination of preharvest corn in Georgia a six-year study of insect damage and visibleAspergillus flavus. J Environ Qual 14: 200–202CrossRefGoogle Scholar
  5. 5.
    Dowd P (2003) Insect management to facilitated preharvest mycotoxins management. J Toxicol-Toxin Rev 22: 327–350Google Scholar
  6. 6.
    Zummo N, Scott G (1989) Evaluation of field inoculation techniques for screening maize genotypes against kernel infection byAspergillus flavus in Mississippi. Plant Dis 73: 313–316CrossRefGoogle Scholar
  7. 7.
    Windham G, Williams W, Buckley P, Abbas H (2003) Inoculation techniques used to quantify aflatoxin resistance in corn. J Toxicol-Toxin Rev 22: 313–325Google Scholar
  8. 8.
    Scott G, Zummo N (1988) Sources of resistance to maize kernel infection byAspergillus flavus in the field. Crop Sci 28: 504–507Google Scholar
  9. 9.
    Scott G, Zummo N (1990) Preharvest kernel infection byAspergillus flavus for resistant and susceptible maize hybrids. Crop Sci 30: 381–383Google Scholar
  10. 10.
    Scott G, Zummo N (1994) Kernel infection and aflatoxin production in maize byAspergillus flavus relative to inculation and harvest dates. Plant Dis 78: 123–125Google Scholar
  11. 11.
    Williams W, Windham G, Buckley P (2003) Enhancing maize germplasm with resistance to aflatoxin contamination. J Toxicol-Toxin Rev 22: 175–193Google Scholar
  12. 12.
    Scott G, Zummo N (1990) Registration of Mp313E parental line of maize. Crop Sci 30: 1378Google Scholar
  13. 13.
    Williams W, Windham G (2001) Registration of dent corn germplasm line Mp715. Crop Sci 41: 1374–1375Google Scholar
  14. 14.
    Windham G, Williams W (2002) Evaluation of corn inbreds and advance breeding lines for resistance to aflatoxin contamination in the field. Plant Dis 86: 232–234CrossRefGoogle Scholar
  15. 15.
    Truckess M, Stack M, Nesheim S, Page S, Albert R, Hansen T, Donahue (1991) Immuno-affinity column coupled with solution fluorometry or liquid chromatography postcolumn derivatization for determination if aflatoxins in corn, peanuts, and peanut butter: collaborative study. J Assoc Off Anal Chem 74: 81–88Google Scholar
  16. 16.
    Williams P, Windham G (2006) Registration of maize germplasm line Mp717. Crop Sci 46: In press.Google Scholar
  17. 17.
    Brooks T, Williams W, Windham G, Willcox M, Abbas H (2005) Quantitative trait loci contributing resistance to aflatoxin accumulation in maize inbred Mp313E. Crop Sci 45: 171–174CrossRefGoogle Scholar
  18. 18.
    Williams W, Davis F, Windham G, Buckley P (2002) Southwestern corn borer damage and aflatoxin accumulation in a diallel cross of maize. J Genet & Breed 56: 165–169Google Scholar
  19. 19.
    Williams W, Windham G, Buckley P (2003) Aflatoxin accumulation in maize after inoculation withAspergillus flavus and infestation with southwestern corn borer. J Genet & Breed 57: 365–370Google Scholar
  20. 20.
    Williams W, Windham G, Buckley P, Daves C (2002) Aflatoxin accumulation in conventional and transgenic corn hybrids infested with southwestern corn borer (Lepidoptera: Crambidae). J Agric Urban Entomol 19: 227–236Google Scholar
  21. 21.
    Williams W, Windham G, Buckley P, Perkins J (2005) Southwestern corn borer damage and aflatoxin accumulation in conventional and transgenic corn hybrids. Field Crops Res 91: 329–336CrossRefGoogle Scholar

Copyright information

© Society of Mycotoxin Research and Springer 2006

Authors and Affiliations

  1. 1.USDA-ARS Corn Host Plant Resistance Research UnitMississippi StateUSA

Personalised recommendations