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Mycotoxin Research

, Volume 23, Issue 4, pp 180–184 | Cite as

Production of zearalenone-4-glucoside, a-zearalenol-4-glucoside and ß-zearalenol-4-glucoside

  • P. Krenn
  • F. Berthiller
  • W. Schweiger
  • C. Hametner
  • R. Ludwig
  • G. Adam
  • R. Krska
  • R. Schuhmacher
Article

Abstract

The work at hand describes the production of the zearalenone (ZON) metabolites zearalenone-4-glucoside (ZON-4G), a-zearalenol-4-glucoside (oc-ZOL-4G) and ß-zearalenol-4-glucoside (ß-ZOL-4G). In a first step a genetically modified yeast strain, expressing theArabidopsis thaliana UDP-glu-cosyltransferase UGT73C6, was treated with ZON to produce ZON-4G. The substance was purified by solid phase extraction and subsequent reversed phase preparative HPLC prior to the reduction with sodium borohydride to yield 0C-ZOL-4G and ß-ZOL-4G. The identity and purity of the substances were confirmed by13C-and1H-NMR as well as by HPLC-UV. In total, 50 mg of ZON were used to produce 5 mg of a-ZOL-4G with a purity of 98%, 6 mg of ß-ZOL-4G with a purity of 99% and 5 mg of ZON-4G with a purity of 99%.

Keywords

α-zearalenol-4-ß-D-glucopyranoside ß-zearalenol-4-ß-D-glucopyranoside zearalenone-4-ß-D-glucopyranoside masked mycotoxins 

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Copyright information

© Society of Mycotoxin Research and Springer 2007

Authors and Affiliations

  • P. Krenn
    • 1
  • F. Berthiller
    • 1
  • W. Schweiger
    • 2
  • C. Hametner
    • 3
  • R. Ludwig
    • 4
  • G. Adam
    • 2
  • R. Krska
    • 1
  • R. Schuhmacher
    • 1
  1. 1.Christian Doppler Laboratory for Mycotoxin Research, Dept. for Agrobiotechnology (IFA-Tulln)University of Natural Resources and Applied Life Sciences ViennaTullnAustria
  2. 2.Inst. of Applied Genetics and Cell Biology, Dept. of Applied Plant Sciences and Plant BiotechnologyUniversity of Natural Resources and Applied Life Sciences ViennaViennaAustria
  3. 3.Inst. for Applied Synthetic ChemistryVienna University of TechnologyViennaAustria
  4. 4.Division of Food Biotechnology, Dept. of Food Sciences and TechnologyUniversity of Natural Resources and Applied Life Sciences ViennaViennaAustria

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