Advertisement

Sugar Tech

, Volume 1, Issue 3, pp 82–88 | Cite as

Role of growth regulators in meristem culture and production of virus-free sugarcane germplasm

  • R. Visessuwan
  • P. Chiemsombat
  • K. Naritoom
  • M. Surijachaijakorn
Article

Abstract

The effects of growth regulators on callus induction and direct shoot induction which derived from meristem culture in length of 0.3, 0.5, 0.7 mm were studied in 12 cultivars of sugarcane. Calli induction in all cultivars were occurred from meristem after culturing onto solid Murashige and Skoog’s (MS) medium containing 3 mg/l 2,4-0 and 10% coconut water (by volume) for 2 months, while direct shoots occurrence was induced from each meristem (0.5 - 0.7 mm in length) in liquid MS medium containing 0.2 mg/l benzyladenine (BA) and 0.02 mg/l napthalene acetic acid (NAA). Calli could differentiate into shoots and developed to plantlets on MS media at reduced concentration of 2, 4-D to 1 mg/l for 2 months of culture. The plantlets were cultured on solid MS medium containing 0.08 mg/l adenine sulfate, 2 mg/l kinetin, 1 mg/l indole butyric acid (IBA) and 10% coconut water for tiller stimulation. An average of 11 shoots/ plantlet were obtained in 2 months. About 80 - 100% of sugarcane plantlets derived from meristem culture of each sugarcane cultivar were free of sugarcane mosaic virus (SCMV) as determined by enzyme-linked immunosorbent assay (ELISA). Plantlets were transferred to MS media containing 4.5% sucrose for root induction and transplanting into soil. Mass propagation and virus-free sugarcane germplasm can be produced by these methods.

Key Words

Growth regulators meristem culture virus-free germplasm sugarcane 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ammirato, P.V. (1986). Control and expression of morphogenesis in culture.In : Plant tissue culture and its agricultural applications. LA. Withers and P.G. Alderson (eds). p. 23–45.CrossRefGoogle Scholar
  2. Clark, M.F. and Adams, A.N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant virus. J. Gen. Virol.,34: 475–483.CrossRefGoogle Scholar
  3. Dean, J.L. (1982). Failure of sugarcane mosaic virus to survive in cultured sugarcane tissue. Plant Disease,66: 1060–1061.CrossRefGoogle Scholar
  4. Flick, C.E., Evans, D.A. and Sharp, W.R. (1983). Organogenesis. p. 13–81.In: Handbook of plant cell culture. Volume 1. D.A. Evans, W.R. Sharp, P.V. Ammirato and Y. Yamada (eds), Macmillan, New York.Google Scholar
  5. Futrakul, A. (1989). Serological method for the detection of sugarcane mosaic virus in sugarcane callus and plantlet derived from tissue culture. MS Thesis, Kasetsart University, Thailand. 85 pp.Google Scholar
  6. Hendre, P.R., Mascarenhas, A.F., Nadgir, A.L., Pathab, M. and Pagahathan, V. (1975). Growth of mosaic-virus-free sugarcane plants from apical meristems. Indian Phytopathol.,28: 175–178.Google Scholar
  7. Langhans, R.W., Horst, R.K. and Eark, E.D. (1977). Disease-free plants via tissue culture propagation. Hort. Science,12: 149–150.Google Scholar
  8. Leu, L.S. (1972). Freeing sugarcane from mosaic virus by apical meristem culture and tissue. Taiwan Sugar Exp. Stn. Rept.,57: 57–63.Google Scholar
  9. Maretzki, A. and Hiraki, P. (1981). Sucrose promotion of root formation in plantlets regenerated from callus ofSaccharum spp. Hort. Abst,51: 758.Google Scholar
  10. Mellore, F.C. and Stace-Smith, R. (1977). Virus-free potatoes by tissue culture, p. 616–635. In J. Reinert and Y.P.S. Bajaj (eds.) Plant cell, tissue and organ culture. Springer-Verlag, Berlin.Google Scholar
  11. Murashige, T. and Skoog, F. (1962). A revised me- dium for rapid growth and bioassays with tabacco tissue cultures. Plant Physiol.,15: 473–497.CrossRefGoogle Scholar
  12. Nadar, H.M., Soepraptapo, S., Heinz, D.J. and Hadd, S.L. (1978). Fine structure of sugarcane (Saccharum sp.) callus and the role of auxin in embryogenesis. Crop Sei.18: 210–216.CrossRefGoogle Scholar
  13. Pierik, R.L.M. (1987). Vegetative propagation. In :In vitro culture of higher plants, p. 183–230. Martinus Nijhoff Publishers, Netherlands.CrossRefGoogle Scholar
  14. Quak, F. (1977). Meristem culture and virus-free plant, p. 598–615. In: J. Reinert and Y.P.S. Bajaj (eds). Plant cell, tissue and organ culture, Springer Verlag, Berlin.Google Scholar
  15. Skoog, F. and Miller, C.O. (1957). Chemical regulation of growth and organ formation in plant tissue, Symp. Soc. Exptl. Biol.,11: 118–131Google Scholar
  16. Scully, R.M. (1967). Aspects of meristem culture in Cattleya alliance. Amer. Orchid Soc. Bull.,36: 103–108.Google Scholar
  17. Thiamann, K.V. (1977). Apical dominance, p. 289–326. In : Hormone action in the whole life of plants, University of Massachusett Press, USA.Google Scholar

Copyright information

© Society for Sugar Research & Promotion 1999

Authors and Affiliations

  • R. Visessuwan
    • 1
  • P. Chiemsombat
    • 2
  • K. Naritoom
    • 1
  • M. Surijachaijakorn
    • 1
  1. 1.Plant Tissue Culture Unit, Central Laboratory and Greenhouse Complex KURDIKasetsart University, Kamphaeng SaenNakhon PathomThailand
  2. 2.Department of Plant Pathology, Faculty of AgricultureKasetsart University, Kamphaeng SaenNakhon PathomThailand

Personalised recommendations