Advertisement

Sugar Tech

, Volume 5, Issue 1–2, pp 37–43 | Cite as

Talc formulated fluorescent pseudomonads for sugarcane red rot suppression and enhanced yield under field conditions

  • N. Senthil
  • T. Raguchander
  • R. Viswanathan
  • R. Samiyappan
Sugar Tech Sugarcane

Abstract

Plant growth promoting rhizobacteria (PGPR) belonging to fluorescent pseudomonads group were isolated from sugarcane rhizosphere and sugarcane stalk tissue. Induction of systemic resistance againstColletotrichum falcatum Went causing red rot disease in sugarcane by PGPR strains were studied under field conditions. The PGPR formulation was applied three times, sett treatment while planting and soil application twice in the field. Talc formulation of five PGPR strains significantly reduced red rot disease incidence when the treated canes were challenge inoculated with pathogen. When PGPR strains were evaluated for their efficacy against the disease in endemic locations, strains ofPseudomonas fluorescens such as EP1, Pf1 and CHAO andP. putida KKM1 strongly suppressed the red rot disease development in two field trials. In addition to their efficacy against red rot disease in sugarcane, the strains significantly improved sett germination, number of millable canes (NMC) and cane yield in the field. Analysis of cane juice characters revealed that PGPR treatments have positively improved juice characters viz., sucrose %, brix % and commercial cane sugar % as compared to the control in the field. The efficacy of PGPR strains against red rot pathogen, enhanced cane and sugar yields suggest that these bacterial strains could be exploited for management of red rot disease in sugarcane.

Key words

Sugarcane induced systemic resistance growth promotion Pseudomonas fluorescens red rot disease 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alexander, K.C. and Viswanathan, R. (1996). Major diseases affecting sugarcane production in India and recent experiences in quarantine. In: Croft, B.J., Piggin, CM., Wallis, E.S., Hogarth, D.M. (ed.)Sugarcane Germplasm Conservation and Exchange. p. 46–48. Proc. ACIAR67, Canberra, Australia.Google Scholar
  2. Duffy, B.K. and Weller, D. M. (1995). Use ofGauemannomyces graminis var.graminis alone and in combination with fluorescentPseudomonas spp. to suppress take-all of wheat.Pl. Dis.79: 907- 911.CrossRefGoogle Scholar
  3. Glick, B.R. (1995). The enhancement of plant growth by free living bacteria.Can. J. Microbiol.4: 109–117.CrossRefGoogle Scholar
  4. Hallmann, J., Quadt-Hallmann, A., Mahaffeee, W.F. and Klocpper, J.W. (1997). Bacterial endophytes in agricultural crops.Can. J. Microbiol.43: 895–914, 1997.CrossRefGoogle Scholar
  5. Hildebrand, D.C., Schroth, M.N. and Sands, D.C. (1992).Pseudomonas-In: Schaad, N.W. (ed.)Laboratory Guide for Identification of Plant Pathogenic Bacteria. 2nd edition. American Phytopathological Society. St. Paul. USA.Google Scholar
  6. Jeyarajan, R., Ramakrishnan, G., Dinakaran, D. and Sridar, R. (1994). Development of products ofTrichoderma viride andBacillus subtilis for biocontrol of root rot diseases. In: Dwivedi (ed.):Biotechnology in India, p. 25–36, Bioved Research Society, Allahabad.Google Scholar
  7. King, E.O., Ward, M.K. and Raney, D.E. (1954). Two simple media for the demonstration of pyocyanin and fluorescein.J. Lab. Clin. Med.44 ; 301–307.Google Scholar
  8. Klocpper, J. W., Tuzun, S., Liu, L. and Wei, G. (1993). Plant growth promoting rhizobacteria as inducers of systemic resistance in plants. In: Lumsden, R.D., Vaughn, J. L. (ed.)Pest Management: Biologically Based Technologies, p156–165. Am. Chem. Soc., Washington.Google Scholar
  9. Klocpper, J.W., Scher, F.M., Laliberte, M. and Tipping, B. (1986). Emergence promoting rhizobacteria: Description and implications for agriculture. In: Swinburne, T.R. (ed.).Iron, Siderophore and Plant Diseases, p 155–164. Plenum Pub. Co.. New York.CrossRefGoogle Scholar
  10. Maurhofer, M., Hase, C., Meuwly, P., Metraux, J.P. and Defago, G. (1994). Induction of systemic resistance of tobacco to tobacco necrosis virus by the root colonizingPseudomonas fluorescens strain CHAO: Influence of thegacA gene and of pyoverdine production.Phytopathology,84: 139–146.CrossRefGoogle Scholar
  11. M’Piga, P., Belanger, R.R., Paulitz, T.C. and Benhamou, N. (1997). Increased resistance toFusarium oxysporum f.sp.radicislycopersici in tomato plants treated with endophytic bacteriumPseudomonas fluorescens strain 63–28.Physiol. Mol. Pl. Pathol.50: 301–320.CrossRefGoogle Scholar
  12. Nandakumar, R., Babu, S., Viswanathan, R., Raguchander, T., and Samiyappan, R. (2001). Induction of systemic resistance in rice against sheath blight disease byPseudomonas fluorescens.Soil Biology and Biochemistry,33: 603–612.CrossRefGoogle Scholar
  13. Rao, K.C. (1986). Analysis of sugarcane for maturity and quality. In:Selection procedures in sugarcane varietal testing. p 12–14. Sugarcane Breeding Institute. Coimbatore, India.Google Scholar
  14. Senthil, N., Jayaraj, J. and Shanmugam, V. (2000). Evaluation of fungal and bacterial antagonists againstColletotrichum falcatum Went, causing red rot of sugarcane.Indian Sugar50: 423–432.Google Scholar
  15. Singh, K. and Singh, R.P. (1989). Red rot. In: Ricaud. C., Egan B.T., Gillaspie, Jr. A.G., Hughes, C.G. (ed.):Diseases of Sugarcane: Major Diseases. p 169–188. Elsevier, Amsterdam.CrossRefGoogle Scholar
  16. Singh, N. (1994).Trichoderma harzianum andChaetomium sp. as potential biocontrol fungi in management of red rot disease of sugarcane.J. Biol. Control.8: 65–67.Google Scholar
  17. Singh, O.N. and Waraitch, K.S. (1977). Metabolic changes induced byColletotrichum falcatum Went in sugarcane.Sugarcane Pathologist’s Newsl.19: 7–9.Google Scholar
  18. Srinivasan, K.V. and Bhat, N.R (1961). Red rot of sugar cane: Criteria for grading resistance.J. Indian Bot. Soc.40: 566–577.Google Scholar
  19. Sundara, B. (1998). Sugarcane cultivation, p. 392.-Vikas Publishing House Pvt Ltd, New Delhi. India.Google Scholar
  20. Van Loon, L.C., Bakker, P.A.H.M. and Pieterse, C.M.J. (1998). Systemic resistance induced by rhizosphere bacteria.Ann. Rev. Phytopathol.36: 453–483.CrossRefGoogle Scholar
  21. Van Peer, R., Niemann, G.J. and Schippers, B. (1991). Induced resistance and phytoalexin accumulation in biological control ofFusarium wilt of carnation byPseudomonas sp. strain WCS417.Phytopathology,81: 728–743.CrossRefGoogle Scholar
  22. Vidhyasekaran, P. and Muthamilan, M. (1995). Development of formulation ofPseudomonas fluorescens for control of chickpea wilt.Pl. Dis.79: 782–780.CrossRefGoogle Scholar
  23. Viswanathan, R., Padmanaban, P. and Mohanraj, D. (1997). Growing virulence of red rot pathogen of sugarcane in Tamil Nadu.Indian Sugar,37: 23–30.Google Scholar
  24. Viswanathan, R. and Samiyappan, R. (1999). Induction of systemic resistance by plant growth promoting bacteria against red rot disease caused byColletotrichum falcatum Went, in sugarcane. Proc. of Sugar Technology Association of India,61: 24–39.Google Scholar
  25. Viswanathan, R. and Samiyappan, R. (2000a). Red rot disease in sugarcane: Challenges and Prospects.Madras Agric. J.87: 549- 559.Google Scholar
  26. Viswanathan, R. and Samiyappan, R. (2001). Role of chitinases inPseudomonas spp. induced systemic resistance againstColletotrichum falcatum in sugarcane.Indian Phytopath., 54: 418- 423.Google Scholar
  27. Viswanathan, R. and Samiyappan, R. (2002). Induced systemic resistance by fluorescent pseudomonads against red rot disease of sugarcane caused byColletotrichum falcatum.Crop Protection,21: 1–10.CrossRefGoogle Scholar
  28. Wei, G., Kloepper, J. W. and Tuzun, S. (1996). Induction of systemic resistance in cucumber diseases and increased plant growth by plant growth promoting rhizobacteria under field conditions.Phytopathology,86: 221–224.CrossRefGoogle Scholar

Copyright information

© Society for Sugar Research & Promotion 1999

Authors and Affiliations

  • N. Senthil
    • 1
  • T. Raguchander
    • 2
  • R. Viswanathan
    • 3
  • R. Samiyappan
    • 1
  1. 1.Department of Plant PathologyCentre for Plant Protection Studies, Tamil Nadu Agricultural UniversityCoimbatore-641 003, Tamil NaduIndia
  2. 2.Sugarcane Research StationTamil Nadu Agricultural UniversityCuddalore-607 001, Tamil NaduIndia
  3. 3.Plant Pathology SectionSugarcane Breeding InsituteCoimbatore-641 007, Tamil NaduIndia

Personalised recommendations