Infectivity of entomopathogenic nematodesHeterorhabditis andSteinernema spp. to sugarcane shoot borer (Chilo infuscatellus snellen) at different temperatures
- 99 Downloads
Infectivity of twenty nine entomopathogenic nematode (EPN) isolates belonging to the families of Heterorhabditidae and Steinernematidae to sugarcane shoot borerChilo infuscatellus Snellen was investigated at 18 and 27°C under laboratory conditions. Although all the EPN isolates tested caused mortality of shoot borer larvae, there were differences in the level of mortality. Among the EPN isolates tested at 27°C, 120 h after inoculationHeterorhabditis indica (LN2),H. bacteriophora (German isolate) andSteinernema glaseri (Australian isolate) caused 100 per cent mortality of shoot borer larvae. Average time taken to kill shoot borer larvae was least (22.8 h) in case ofH. indica (LN2) followed byS. glaseri (26.5 h) andH. bacteriophora (German isolate) (28.4 h). Mortality percentage for other isolates ranged from 39.9 to 86.6. At 18°C,S. glaseri (Australian isolate) andH. bacteriophora (German isolate) caused 100 per cent mortality 120 h after nematode inoculation; with an average time taken to kill shoot borer larvae of 41.2 and 42.6 h respectively. All the EPN isolates took less time to cause mortality of shoot borer larvae at 27 than at 18°C. The multiplication of EPN in shoot borer larvae infected at 27°C was comparatively more than those infected at 18°C.
KeywordsBiological control Chilo infuscatellus Entomopathogenic nematodes Nematode reproduction Sugarcane
Unable to display preview. Download preview PDF.
- Avasthy, P.N. and Tiwari, N.K. (1986). The shoot borer,Chilo infuscatellus Snellen P. 69–82. In: H. David, S. Easwaramoorthy and R. Jayanthi (eds). Sugarcane Entomology in India, Sugarcane Breeding Institute, Coimbatore.Google Scholar
- David, H. and Kurup, N.K. (1988). Techniques for mass production ofSturmiopsis inferens Tns. p. 87–92. In: H. David and S. Easwaramoorthy (eds).Biocontrol technology for Sugarcane Pest Management. Sugarcane Breeding Institute, Coimbatore.Google Scholar
- Easwaramoorthy, S., David, H., Shanmugasundaram, M. and Santhalakshmi, G. (1991). Seasonal occurrence of the tachinid parasite,Sturmiopsis inferens Tns. in the sugarcane agro ecosystem.Journal of Biological Control,5: 1–3.Google Scholar
- Ehlers, R.U., Niemann, I., Hollmer, S., Strauch, O., Jende, D., Shanmugasundaram, M., Mehta, U.K., Easwaramoorthy, S. and Burnell, A.M. (2000). Mass production potential of the bacto-helminthic biocontrol complexHeterorhabditis indica-Photorhabdus luminescens.Biocontrol Science and Technology,10: 607–616.CrossRefGoogle Scholar
- Kalyanaraman, V.M., Leela David, A. and Narayanaswamy, P.S. (1963). Distribution, seasons of occurrence and control of the early shoot borer of sugarcane,Chilo infuscatellus Snell. in Madras State.Indian Journal of Sugarcane Research and Development,7: 89–95.Google Scholar
- Karunakar, G. (1990).Entomophilic nematodes for the control of three species of white grubs in sugarcane. M.Sc. (Ag) Thesis, Tamilnadu Agricultural University, Coimbatore, India.Google Scholar
- Karunakar, G., Easwaramoorthy, S. and David, H. (1999). Susceptibility of nine lepidopteran insects toSteinernema glaseri, S. feltiae andHeterorhabditis indicus infection.International Journal of Nematology,9: 68–71.Google Scholar
- Kaya, H.K. (1990). Soil ecology. In:Entomopathogenic nematodes in biological control. R. Gaugler and H.K. Kaya (eds) Boca Raton, CRC Press, Florida, p. 93–112.Google Scholar
- Woodring, J.L. and Kaya, H.K. (1988).Steinernematid and Heterorhabditid nematodes. A handbook of biology and techniques. Fayetteville, USA.Google Scholar