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Sugar Tech

, Volume 5, Issue 3, pp 149–153 | Cite as

molecular characterization of sugarcane mosaic virus isolate from North Eastern Region of India

  • R. K. Gaur
  • Maneesha Singh
  • G. P. RaoEmail author
Sugarcane

Abstract

An isolate of Sugarcane mosaic virus causing mosaic disease in sugarcane cultivar CoSe 93232 from the north-eastern region of India was molecularly characterized. The size of the RNA was about 9.8 + 0.1 kbp on 1% agarose gel. Immuno-based polymerase chain reaction was performed to amplify the nucleocapsid protein gene with primer pair 5’-CGXATCGYTAZZATNNG-3’and 5’-GCTATGGGCAGATGCCC-3’ The nucleocapsid protein gene was cloned and sequenced. The length of the coat protein was 1330 nucleotides encoding 365 amino acids. This nucleotide amino acid sequence showed closest homology with SCMV-SC isolate from Australia (99%) followed by SCMV-A (USA, 97%), SCMV-BJ (China, 87%) and SCMV-MDB (USA, 80%). The sequence identity and phylogenetic relationship of the Indian virus isolate with characterized SCMV strains from Australia, USA, Egypt, Cameroon, China, South Africa and Congo indicated that this isolate belonged to Sugarcane mosaic potyvirus subgroup. This is the first molecular confirmation of SCMV from India.

Keywords

Sugarcane Potyvirus IC RT-PCR amplification India 

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Copyright information

© Society for Sugar Research & Promotion 1999

Authors and Affiliations

  1. 1.Division of Plant PathologySugarcane Research StationKunraghat, Gorakhpur — 273 008, UPIndia

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