Screening of sugarcane mosaic potyvirus (SCMV) from cane stalk juice
- 144 Downloads
Sugarcane mosaic potyvirus (SCMV) is widely infected in most of the sugarcane growing belts of India causing intervienal chlorotic specks, streaks or stripes especially on young leaves. Earlier only the infected leaves extract were commonly used for the screening of this virus by serological methods. However, in the present study, direct antigen coating enzyme linked immunosorbant assay (DAC-ELISA) and dot blot immunoassay (DIBA) were standardized for the detection of the virus in infected cane stalk juice samples. Juice dilution up to 1:150 was found satisfactory for the detection of SCMV in DAC-ELISA and DIBA serological tests. Our results suggest that cane stalk juice could be equally satisfactory as virus infected leaf samples and can be used in commercial screening of sugarcane mosaic potyvirus.
KeywordsSugarcane mosaic virus screening ELISA DIBA cane stalk juice
Unable to display preview. Download preview PDF.
- Agnihotri, V. P. (1996). Current sugarcane disease scenario and management strategies.Indian Phytopathol.,49: 109–126.Google Scholar
- Clark, M. F. and Joseph, M. Bar. (1984). In : Methods in Virology (Eds. Maramorosch, K. and Koproski, H.) pp 51–85, AP, N4.Google Scholar
- Jain, R. K., Rao, G. P. andVerma, A. (1998). Present status of management of sugarcane mosaic virus, pp. 495–523.In: Plant virus disease control, (A. Hadidi, R. K. Khetarpal and H. Koganezava, (eds), APS Press, Minnesota, USA.Google Scholar
- Rao, G. P., Singh, Maneesha, Rishi, N. andBhargava, K. S. (2002). Century status of sugarcane virus diseases research in India, pp. 223–254.In: Sugarcane Crop Management (Eds S. B. Singh, G. P. Rao and S. Easwaramoorthy) Sci-Tech Publishing, LLC., Texas, USA, pp 734.Google Scholar
- Rishi, N. andRishi, S. (1985). Purification, electron microscopy and serology of strains A and F of sugarcane mosaic virus.Indian Jour. Virology,1: 79–86.Google Scholar
- Singh, Maneesha (2001). Studies on virus diseases of sugarcane with special reference to identification, economic losses and varietal susceptibility. Ph.D Thesis, D.D.U. Gorakhpur University Gorakhpur, India.Google Scholar