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Sugar Tech

, Volume 4, Issue 3–4, pp 169–171 | Cite as

Screening of sugarcane mosaic potyvirus (SCMV) from cane stalk juice

  • R. K. Gaur
  • Maneesha Singh
  • Akhand Pratap Singh
  • A. K. Singh
  • G. P. RaoEmail author
Short Communications

Abstract

Sugarcane mosaic potyvirus (SCMV) is widely infected in most of the sugarcane growing belts of India causing intervienal chlorotic specks, streaks or stripes especially on young leaves. Earlier only the infected leaves extract were commonly used for the screening of this virus by serological methods. However, in the present study, direct antigen coating enzyme linked immunosorbant assay (DAC-ELISA) and dot blot immunoassay (DIBA) were standardized for the detection of the virus in infected cane stalk juice samples. Juice dilution up to 1:150 was found satisfactory for the detection of SCMV in DAC-ELISA and DIBA serological tests. Our results suggest that cane stalk juice could be equally satisfactory as virus infected leaf samples and can be used in commercial screening of sugarcane mosaic potyvirus.

Keywords

Sugarcane mosaic virus screening ELISA DIBA cane stalk juice 

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References

  1. Agnihotri, V. P. (1996). Current sugarcane disease scenario and management strategies.Indian Phytopathol.,49: 109–126.Google Scholar
  2. Clark, M. F. and Joseph, M. Bar. (1984). In : Methods in Virology (Eds. Maramorosch, K. and Koproski, H.) pp 51–85, AP, N4.Google Scholar
  3. Hawkes, R. E., Niday, E. andGordon, J. (1982). A dot immunobinding assay for monoclonal and other antibodies.Anal. Biochemic,119: 142–147.CrossRefGoogle Scholar
  4. Jain, R. K., Rao, G. P. andVerma, A. (1998). Present status of management of sugarcane mosaic virus, pp. 495–523.In: Plant virus disease control, (A. Hadidi, R. K. Khetarpal and H. Koganezava, (eds), APS Press, Minnesota, USA.Google Scholar
  5. Rao, G. P., Singh, Maneesha, Rishi, N. andBhargava, K. S. (2002). Century status of sugarcane virus diseases research in India, pp. 223–254.In: Sugarcane Crop Management (Eds S. B. Singh, G. P. Rao and S. Easwaramoorthy) Sci-Tech Publishing, LLC., Texas, USA, pp 734.Google Scholar
  6. Rishi, N. andRishi, S. (1985). Purification, electron microscopy and serology of strains A and F of sugarcane mosaic virus.Indian Jour. Virology,1: 79–86.Google Scholar
  7. Singh, Maneesha (2001). Studies on virus diseases of sugarcane with special reference to identification, economic losses and varietal susceptibility. Ph.D Thesis, D.D.U. Gorakhpur University Gorakhpur, India.Google Scholar

Copyright information

© Society for Sugar Research & Promotion 1999

Authors and Affiliations

  • R. K. Gaur
    • 1
  • Maneesha Singh
    • 1
  • Akhand Pratap Singh
    • 2
  • A. K. Singh
    • 2
  • G. P. Rao
    • 1
    Email author
  1. 1.Division of Plant PathologySugarcane Research StationKunraghat, Gorakhpur-273 008, UPIndia
  2. 2.Department of BotanyM.L.K. (P.G) CollegeBalarampur-271 201, UPIndia

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