Advertisement

Sugar Tech

, Volume 6, Issue 1–2, pp 41–46 | Cite as

Isolation and characterization of resistance traits of indigenous strains ofAcetobacter diazotrophicus associated with sugarcane

  • Iqbal AhmadEmail author
  • Jyoti Sharma
  • Farah Ahmad
Sugarcane

Abstract

A total of 11 strainsof Acetobacter diazotrophicus were isolated from roots, leaves and stems of the four locally grown cultivars of sugarcane (Co-1148, UP 39, Satha-676, Satha-91269) of Aligarh. These strains and three Brazilian strains ofAcetobacter diazotrophicus (PR2-ATCC49039, PaI5-ATCC. 49037, Ppe-4 ATCC49038) were studied for their biochemical characteristics and resistance traits. All isolated strains exhibited similar cultural and biochemical characteristics to that of reference strains. These strains utilized sucrose, glucose and 1% ethanol while no strain could utilize maltose and 10% ethanol. Fructose was utilized differentially; similarly all strains were positive for catalase, H2S production and negative for oxidase, NO3 reduction, denitrification, gelatin liquefaction and indole test. Antibiotic resistance in theAcetobacter diazotrophicus was exhibited by 12 out of 14 strains. Similarly, metal resistance at MIC. 100 μg/ml was found maximum against Pb followed by Cd, Hg, Ni and Cu. Most of the indigenous strains showed resistance to both antibiotics and heavy metals. However, three Brazilian strains ofAcetobacter diazotrophicus were sensitive to all metals tested. Similarly tolerance to salt (NaCl) was higher 1.0% to 1.5% among indigenous strains as compared to Brazilian strains, which could tolerate NaCl up to 0.5% in the agar medium.

Keywords

Sugarcane Acetobacter diazotrophicus antibiotic and metal resistance salt tolerance 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ahmad, S. and Yadava, J.N.S. (1988). Infectious mercury resistance and its co-transfer with “R-plasmid amongE. coli strains.Indian J. Exp. Biol.,26: 601–605.PubMedGoogle Scholar
  2. Ahmad, I., Kayat, S., Ahmad, A., Inam, A. and Samiuilah (2001). Metal and antibiotic resistance traits in Bradyrhizobium sp. (cajanus) isolated from soil receiving oil refinery waste water.World J. Micrbiol. Biotech.,17: 379–389.CrossRefGoogle Scholar
  3. Bauer, A.W., Kirby, W.M.M., Sherris, J.C. and Turch, M. (1966). Antibiotic susceptibility testing by a standardized single disc method.American Journal of Clinical Pathology,45: 494- 496.CrossRefGoogle Scholar
  4. Cappucino, J.G. and Sherman, N. (1995). Microbiology: A Laboratory Manual 3rd (ed). The Benjamin/Cummings Publishing Company, Inc. Redwood City, California.Google Scholar
  5. Cavalcante, V. and Dobereiner, J. (1988). A new acid- tolerant nitrogen fixing bacterium associated with sugarcane.Plant Soil,108: 23–31.CrossRefGoogle Scholar
  6. Fuentes-Ramirez, L.E., Jimenez-Salgado, T., Abarca-Ocampo, I.R. and Caballero-Mellado, J. (1993).Acetobacter dizotrophicus, an indole acetic producing bacterium isolated from sugarcane cultivars of Mexico.Plant Soil.,154: 145–150.CrossRefGoogle Scholar
  7. Ghosh, A.B., Bajaj, J.C. Hasan, R. and Singh, D. (1983). Soil and water testing methods: A laboratory manual, New Delhi, Indian Agricultural Research Institute.Google Scholar
  8. Jimenez-Saigado, T., Fuentes-Ramirez, L.E., Tapia-Hernandez, A., Mascarua-Esparza, M.A., Martinez-Romero, E., Caballero-Mellado, J. (1997). Coffea arabica L., a new host plant forAcetobacter dizotrophicus, and isolation of other nitrogen fixing Acetobacteria.Applied Environ. Microbiol.,63: 3676–3683.Google Scholar
  9. Li, R.P. and Macrae, I.C. (1991). Specific association of diazotrophicus acetobacters with sugarcane SoilBiol. tBiochem.,23: 999–1002.CrossRefGoogle Scholar
  10. Maheshkumar, K.S., Krishnaraj, P.U. and Algawadi, A.R. (1999). Mineral phosphate solubilizing activityofAcetobacter diazotrophicus: a bacterium associated with sugarcane.Current Science,76: 874–875.Google Scholar
  11. Muthukumarasamy, R., Revathi, G., Seshadri, S. and Lakshminarsimhan, C. (2002). Gluconoacetobacter diazotrophicus (syn.Acetobacter diazotrophicus), a promising diazotrophic endophyte in tropics.Current Science,83(2): 137- 145.Google Scholar
  12. Muthukumarasamy, R., Revathi, G. and Vadivelu, M. (2000). Antagonistic potential of nitrogen fixingAcetobacter diazotrophicus againstColletotrichum falcatum Went., a causal organism of red- rot of sugarcane.Current Science,78(9): 1063- 1065.Google Scholar
  13. Sevilla, M., de Oliveira, A. Baldani, I. and Kennedy, C. (1998). Contribution of the bacterial endophytesAcetobacter diazotrophicus to sugarcane; a preliminary study.Symbiosis,25: 181–191.Google Scholar

Copyright information

© Society for Sugar Research & Promotion 1999

Authors and Affiliations

  1. 1.Department of Agricultural MicrobiologyRAK, Institute of Agricultural Sciences, Aligarh Muslim UniversityAligarhIndia

Personalised recommendations