Minimizing phenol pollution in sugarcane stem apical meristem culture
The stem apical meristem expiants of sugarcane were pretreated with sterile water for 30 minutes and inoculated on MS medium with 6 BA 2.0 mg.L-1 + NAA 0.1 mg.L-1. The best result of minimizing phenol pollution was obtained when 0.02% (not higher than 0.05%) active carbon (AC) was directly added into the media. The phenol pollution could also be minimized when antioxidant PVP (Polyvinylpyrrolidone) was added into the media, or the explants were transferred onto fresh medium when they began getting brown.
KeywordsSugarcane Apical culture Phenol pollution
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