Abstract
Microbial oxidation of D-sorbitol tol-sorbose byAcetobacter suboxydans is of commercial importance since it is the only biochemical process in vitamin C synthesis. The main bottleneck in the batch oxidation of sorbitol to sorbose is that the process is severely inhibited by sorbitol. Suitable fed-batch fermentation designs can eliminate the inherent substrate inhibition and improve sorbose productivity. Fed-batch sorbose fermentations were conducted by using two nutrient feeding strategies. For fed-batch fermentation with pulse feeding highly concentrated sorbitol (600 g/L) along with other nutrients were fed intermittently in four pulses of 0.5 liter in response to the increased DO signal. The fed-batch fermentation was over in 24 h with a sorbose productivity of 13.40 g/L/h and a final sorbose concentration of 320.48 g/L. On the other hand, in fed-batch fermentation with multiple feeds, two pulse feeds of 0.5 liter nutrient medium containing 600 g/L sorbitol was followed by the addition of 1.5 liter nutrient medium containing 600 g/L sorbitol at a constant feed rate of 0.36 L/h till the full working capacity of the reactor. The fermentation was completed in 24 h with an enhanced sorbose productivity of 15.09 g/L/h and a sorbose concentration of 332.60 g/L. The sorbose concentration and productivity obtained by multiple feeding of nutrients was found to be higher than that obtained by pulse feeding and was therefore a better strategy for fed-batch sorbose fermentation.
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References
Bourdant, J. (1990) Microbial processes for ascorbic acid synthesis: A review.Enzyme Microb. Technol. 12: 322–329.
Damodaran, M. and S. S. Subramanian (1951) Bacterial oxidation of sorbitol to sorbose.J. Sci. Ind. Res. 5(10): 7–13.
Bonomi, A., E. F. P. Augusto, N. S. Barbosa, M. N. Mattos, L. R. Magossi, and A. L. Santos (1993) Unstructured model proposal for the microbial oxidation of D-Sorbitol to L-Sorbose.J. Biotechnol. 31: 39–59.
Srivastava, A. K. and P. R. Lasrado (1998) Fed batch sorbitol to sorbose fermentation byA. suboxydans.Bioproc. Eng. 18: 457–461.
Mori, H., T. Kobayshi, and S. Shimizu (1981) High density production of sorbose from sorbitol by fed batch culture with DO-stat.J. Chem. Eng. Jpn. 14: 65–70.
Bosjnak, M., P. Pintar, and K. Gomercic (1991) Gradient fed-batch culture as efficient microbial oxidation method.Med. Fac. Landbouww. Rijksuniv. Gent. 56: 1723–1730.
Lee, S. Y. (1996) High cell-density culture ofEscherichia coli: Reviews.TIBTECH 14: 98–105.
Yano, T., M. Kurokawa, and Y. Nishizawa (1991) Optimum substrate feed rate in fed-batch culture with the DO-stat method.J. Ferment. Bioeng. 71: 345–349.
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Giridhar, R., Srivastava, A.K. Fed-batch sorbose fermentation using pulse and multiple feeding strategies for productivity improvement. Biotechnol. Bioprocess Eng. 5, 340–344 (2000). https://doi.org/10.1007/BF02942209
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DOI: https://doi.org/10.1007/BF02942209