Production of ethanol from enzymatically hydrolyzed orange peel by the yeastSaccharomyces cerevisiae

  • Karel Grohmann
  • Elizabeth A. Baldwin
  • Béla S. Buslig
Session 2 Applied Biological Research


We extended our previous investigations of enzymatic hydrolysis of polysaccharides in orange peel by commercial cellulase and pectinase enzymes to higher, more practical concentrations of orange peel solids. High yields of saccharification could be maintained even at substrate concentrations as high as 22–23%, but the rates of solubilization and saccharification decreased 2-3-fold. We also tested the fermentability of these hydrolysates by the yeastSaccharomyces cerevisiae, which revealed the presence of inhibitory compounds. These compounds could be removed by the filtration of hydrolyzed peel. Successful fermentations of filtered hydrolysates were achieved after pH adjustment with calcium carbonate.

Index Entries

Orange peel carbohydrates enzymatic hydrolysis fermentation Saccharomyces cerevisiae 


  1. 1.
    Agricultural Research Service (1962),Chemistry and Technology of Citrus, Citrus Products and Byproducts. Agriculture Handbook No. 98, United States Department of Agriculture Publ., Washington, DC, p. 98.Google Scholar
  2. 2.
    Kesterson, J. W. and Braddock, R. J. (1976),By-Products and Specialty Products of Florida Citrus. Bulletin #784, University of Florida Publ., Gainesville, FL, p. 119.Google Scholar
  3. 3.
    Grohmann, K. and Baldwin, E. A. (1992),Biotechnol. Lett. 14, 1169–1174.CrossRefGoogle Scholar
  4. 4.
    Nishio, N., Oku, Y., Kawamara, D., and Nagai, S. (1979),Hakko Kogaku Kaishi 57, 354–359.Google Scholar
  5. 5.
    Nishio, N. and Nagai, S. (1979),Eur. J. Appl. Microbiol. Biotechnol. 6, 371–378.CrossRefGoogle Scholar
  6. 6.
    Marshall, M. R., Graumlich, T. R., Braddock, R. J., and Messersmith, M. (1985),J. Food Sci. 50, 1211,1212.CrossRefGoogle Scholar
  7. 7.
    Echeverria, E., Burns, J. K., and Wicker, L. (1988),Proc. Fla. State Hort. Soc. 101, 150–154.Google Scholar
  8. 8.
    Akao, T., Mizuki, E., Saito, H., Okumura, S., and Murao, S. (1992),Bioresource Technol. 41, 35–39.CrossRefGoogle Scholar
  9. 9.
    Ben-Shalom, N. (1986),J. Food Sci. 51, 720,721, 730.CrossRefGoogle Scholar
  10. 10.
    Nelson, N. (1944),J. Biol. Chem. 153, 375–386.Google Scholar
  11. 11.
    Kohn, R. and Kovac, P. (1978),Chem. Zvesti 32, 478–485.Google Scholar
  12. 12.
    Grohmann, K., Wyman, C. E., and Himmell, M. E. (1992), inEmerging Technologies for Materials and Fuels from Biomass, Rowell, R. M., Schulz, T. P., and Narayan, R., eds., ACS Symp. Series No. 476, American Chemical Society Publ., Washington, DC, pp. 354–392.Google Scholar
  13. 13.
    Tong, G. E. and Cannell, R. P. (1988), inOrganic Chemicals from Biomass, Wise, D. L., ed., Benjamin/Cummings, Menlo Park, CA, pp. 407–451.Google Scholar
  14. 14.
    Rohatgi, N. K. and Ingham, J. D. (1992),Appl. Biochem. Biotechnol. 34/35, 515–526.CrossRefGoogle Scholar
  15. 15.
    Von Loesecke, H. W. (1934),Citrus Ind. 15, 8,9, 20,21.Google Scholar
  16. 16.
    McNary, R., Wolford, R. W., and Patton, V. D. (1951),Food Technol. 5, 319–323.Google Scholar
  17. 17.
    Lane, A. G. (1980),J. Chem. Tech. Biotechnol. 30, 345–350.Google Scholar
  18. 18.
    Lane, A. G. (1983),Environ. Technol. Lett. 4, 65–72.CrossRefGoogle Scholar
  19. 19.
    Mizuki, E., Akao, T., and Saruwatari, T. (1990),Biol. Wastes 33, 161–168.CrossRefGoogle Scholar
  20. 20.
    Murdock, D. I. and Allen, W. E. (1960),Food Technol. 14, 441–445.Google Scholar
  21. 21.
    Subba, M. S., Soumithri, T. C., and Suryanarayana, R. (1967),J. Food Sci. 32, 225–227.CrossRefGoogle Scholar
  22. 22.
    Shaw, P. E. (1979),J. Agric. Food Chem. 27, 246–257.CrossRefGoogle Scholar
  23. 23.
    Scott, W. C. and Veldhuis, M. K. (1966),J. A.O.A.C. 49, 628–633.Google Scholar
  24. 24.
    Barnett, J. A., Payne, R. W., and Yarrow, D. (1985), inYeasts Characteristics and Identification, Cambridge University Press, Cambridge, UK, p. 811.Google Scholar
  25. 25.
    Carlson, M. (1987),J. Bacterial. 169, 4873–4877.Google Scholar
  26. 26.
    Hara, T., Fujio, Y., and Ueda, S. (1985),Nippon Shokahin Kogyo Gakkaishi 32, 241–246.Google Scholar
  27. 27.
    Sinclair, W. B. (1961), inThe Orange. Its Biochemistry and Physiology, Sinclair, W. B., ed., Univ. of California, Publ., Riverside, CA, pp. 191–229.Google Scholar

Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • Karel Grohmann
    • 1
  • Elizabeth A. Baldwin
    • 1
  • Béla S. Buslig
    • 2
  1. 1.Citrus & Subtropical Products LaboratoryUSDAWinter Haven
  2. 2.Florida Department of CitrusWinter Haven

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