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Applied Biochemistry and Biotechnology

, Volume 45, Issue 1, pp 245–256 | Cite as

A new thermostable endoglucanase,Acidothermus cellulolyticus E1

Synergism withTrichoderma reesei CBH I and comparison toThermomonospora fusca E5
  • John O. Baker
  • William S. Adney
  • Rafael A. Nleves
  • Steven R. Thomas
  • David B. Wilson
  • Michael E. Himmel
Session 2 Applied Biological Research

Abstract

A new thermostable endoglucanase,Acidothermus cellulolyticus E1, and another bacterial endoglucanase, E5 fromThermomonospora fusca, each exhibit striking synergism with a fungal cellobiohydrolase (Trichoderma reesei CBH I) in the saccharification of microcrystalline cellulose. In neither case did the ratio of endoglucanase to exoglucanase that demonstrated maximum synergism coincide exactly with the ratio that actually released the maximum quantity of soluble sugar for a given total cellulase loading. The difference between the two ratios, after significant hydrolysis of the substrate, was considerably larger in the case ofA. cellulolyticus E1. For both endoglucanase pairings with CBH I, the offset between the ratio for maximum synergism and the ratio for maximal soluble sugar production was found to be a function of digestion time.

Index Entries

Acidothermus cellulolyticus E1 Thermomonospora fusca E5 Trichoderma reesei CBH I synergism endoglucanase 

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Copyright information

© Humana Press Inc. 1994

Authors and Affiliations

  • John O. Baker
    • 1
  • William S. Adney
    • 1
  • Rafael A. Nleves
    • 1
  • Steven R. Thomas
    • 1
  • David B. Wilson
    • 2
  • Michael E. Himmel
    • 1
  1. 1.Alternative Fuels DivisionNational Renewable Energy LaboratoryGolden
  2. 2.Section of Biochemistry, Molecular and Cell BiologyCornell UniversityIthaca

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