Mycotoxin Research

, 19:51

Analysis of deoxynivalenol and de-epoxy-deoxynivalenol in animal tissues by liquid chromatography after clean-up with an immunoaffinity column

  • H. Valenta
  • S. Dänicke
  • S. Döll
Article

Abstract

A method for the determination of deoxynivalenol (DON) and its metabolite de-epoxy-deoxynivalenol (de-epoxy-DON) in blood serum, urine, faeces/digesta and bile fluid is described. Liquid samples (urine and bile after incubation with ß-glucuronidase) were extracted with ethyl acetate on a ChemElut column. Freeze dried faeces and digesta were extracted with a mixture of acetonitrile and water. For clean-up, serum and urine extracts could be directly applied to an immunoaffinity column (IAC). A pre-treatment prior to the IAC clean-up was necessary in the case of bile and faeces/digesta. DON and de-epoxy-DON were determined by high performance liquid chromatography (HPLC) with diode-array detection (DAD).

Because of the clean extracts, low detection limits in the range of 4 ng/ml (serum) to 20 ng/g (dried faeces/digesta) were achieved. The recovery of DON and de-epoxy-DON was in the range of 75–89% and 64–85%, respectively. DON was found in blood serum, urine, bile, digesta and faeces of pigs from several feeding studies with DON contaminated feed; de-epoxy-DON could be detected only in digesta, faeces and urine.

Keywords

mycotoxin deoxynivalenol de-epoxy-deoxynivalenol method animal tissues HPLC 

References

  1. 1.
    Dänicke, S., Valenta, H., Ueberschär, K.-H. (2000) in Dänicke, S., Oldenburg, E. (eds.) Risikofaktoren für die Fusariumtoxinbildung in Futtermitteln und Vermeidungsstrategien bei der Futtermittelerzeugung und Fütterung. Landbauforschung Völkenrode, Sonderheft 216: 108–139.Google Scholar
  2. 2.
    Dahlem, A.M., Swanson, S.P., Cote, L.-M., Yoshizawa, T., Buck, W.B. (1986) Quantitation of deoxynivalenol and its metabolite in bovine urine and feces by gas chromatography with electron-capture detection. J. Chromatogr. 378: 226–231.PubMedCrossRefGoogle Scholar
  3. 3.
    Razzazi, E., Böhm, J., Kettner, B., Hochsteiner, W., Kahlbacher, H. (2002) Metabolisierung von Deoxynivalenol beim Schwein: Bestimmung von DON und DOM-1 im Urin vom Schwein. Mycotoxin Res. 18A: 84–88.Google Scholar
  4. 4.
    VDLUFA-Methodenbuch III — 5. Ergänzung 200X, VDLUFA-Verlag, DarmstadtGoogle Scholar
  5. 5.
    Valenta, H., Dänicke, S., Wolff, J. (2002) Vergleich einer HPLC- und einer ELISA-Methode zur Bestimmung von Deoxynivalenol in Mühlenstäuben, Kleien und Getreide. 114. VDLUFA-Kongress, 16.–20.9.2002, Leipzig, VDLUFA-Schriftenreihe, in Druck.Google Scholar
  6. 6.
    Janes, W., Schuster, M. (2001) Determination of Deoxynivalenol in Blood, Bile, Urine and Excrement Samples from Swine Using Immunoaffinity Chromatography and LC-UV-Detection. Mycotoxin Res. 17: 88–95.CrossRefGoogle Scholar
  7. 7.
    Dänicke, S., Goyarts, T., Valenta, H., Razzazi, E. and Böhm, J. (2003) On the effects of increasing deoxynivalenol (DON) concentrations in pig feed on growth performance and utilization of nutrients and on DON metabolism.submitted Google Scholar
  8. 8.
    Döll, S., Dänicke, S., Ueberschär, K.-H., Valenta, H., Schnurrbusch, U., Ganter, M., Klobasa, F., Flachowsky, G. (2003) Effects of graded levels ofFusarium toxin contaminated maize in diets for female weaned piglets. Arch, Anim. Nutr., in press.Google Scholar

Copyright information

© Society of Mycotoxin Research and Springer 2003

Authors and Affiliations

  • H. Valenta
    • 1
  • S. Dänicke
    • 1
  • S. Döll
    • 1
  1. 1.Institute of Animal NutritionFederal Agricultural Research Centre (FAL)BraunschweigGermany

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