Mycotoxin Research

, 19:51

Analysis of deoxynivalenol and de-epoxy-deoxynivalenol in animal tissues by liquid chromatography after clean-up with an immunoaffinity column

  • H. Valenta
  • S. Dänicke
  • S. Döll


A method for the determination of deoxynivalenol (DON) and its metabolite de-epoxy-deoxynivalenol (de-epoxy-DON) in blood serum, urine, faeces/digesta and bile fluid is described. Liquid samples (urine and bile after incubation with ß-glucuronidase) were extracted with ethyl acetate on a ChemElut column. Freeze dried faeces and digesta were extracted with a mixture of acetonitrile and water. For clean-up, serum and urine extracts could be directly applied to an immunoaffinity column (IAC). A pre-treatment prior to the IAC clean-up was necessary in the case of bile and faeces/digesta. DON and de-epoxy-DON were determined by high performance liquid chromatography (HPLC) with diode-array detection (DAD).

Because of the clean extracts, low detection limits in the range of 4 ng/ml (serum) to 20 ng/g (dried faeces/digesta) were achieved. The recovery of DON and de-epoxy-DON was in the range of 75–89% and 64–85%, respectively. DON was found in blood serum, urine, bile, digesta and faeces of pigs from several feeding studies with DON contaminated feed; de-epoxy-DON could be detected only in digesta, faeces and urine.


mycotoxin deoxynivalenol de-epoxy-deoxynivalenol method animal tissues HPLC 


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Copyright information

© Society of Mycotoxin Research and Springer 2003

Authors and Affiliations

  • H. Valenta
    • 1
  • S. Dänicke
    • 1
  • S. Döll
    • 1
  1. 1.Institute of Animal NutritionFederal Agricultural Research Centre (FAL)BraunschweigGermany

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