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Biotechnology and Bioprocess Engineering

, Volume 8, Issue 3, pp 199–204 | Cite as

Screening of antioxidative activity ofBifidobacterium species isolated from Korean infant feces and their identification

  • Ji Youn Kim
  • Soo Im Choi
  • Tae Ryeon Heo
Article

Abstract

Among 59 Korean isolated, 20 were confirmed as members of the genusBifidobacterium species based on gram staining, microscopic examination of cell morphology and the TLC method. The oxygen tolerance and antioxidative activities of these 20Bifidobacterium strains and 5 standardBifidobacterium strains were tested. All the strains demonstrated antioxidative activities as regards inhibiting linoleic acid peroxidation. The antioxidative activities of isolated and standard strains were found to range from 10.7–46.4% and from 10.7–22.2%, respectively. In addition, all tested strains exhibited a scavenging ability on DPPH free radicals, range from 15–41% for the isolated strains and 8.3–22% for the standard strain. Accordingly, the isolatedBifidobacterium strains demonstrated higher antioxidative activities than the 5 standardBifidobacterium strains. On the base of grades for each test, HJL 7511 was identified as the best strain, followed by HJL 7501. 2 strains were identified with Polymerase Chain Reaction (PCR) assay using group-specific primers designed from the nucleotide sequences of the 16S rRNA and internal transcribed spacer (ITS) regions of the Bifidobacteria. Based on the sequencing results, HJL 7511 and HJL 7501 were identified asBifidobacterium infantis.

Keywords

antioxidative activity isolated bifidobacteria 16S rRNA-ITS primers PCR 

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References

  1. [1]
    Benzkorovainy, A. and M. C. Robin (1998)Biochemistry and Physiology of Bifidobacteria. Robin Miller-Catchpole Editor. pp. 73–92. CRC Press, Florida, USA.Google Scholar
  2. [2]
    Gismongo, M. R., L. Drago and A. Lombardi (1999) Review of probiotics available to modify gastrointestinal flora.Int. J. Antimicro. Agents 12: 287–292.CrossRefGoogle Scholar
  3. [3]
    Gilliland, S. E. (1990) Health and nutritional benefits from lactic acid bacteria.FEMS Microbiol. Rev. 87: 175–188.CrossRefGoogle Scholar
  4. [4]
    Homma, N. (1988) Bifidobacteria as are resistance factor in human beings.Bifidobact. Microfl. 7: 35–43.Google Scholar
  5. [5]
    Mitsuoka, T. (1990) Bifidobacteria and their role in human health.J. Ind. Microbiol. 6: 263–268.CrossRefGoogle Scholar
  6. [6]
    Hosono, A., R. Wardojo, and H. Otani (1990) Inhibitory effects of lactic acid bacteria from fermented milk on the mutagenecities of volatile nitrosamines.Agr. Biol. Chem. 54: 1639–1643.Google Scholar
  7. [7]
    Rasic, J. L., I. F. Vujicic, M. Skrinjar, and M. Vulic (1992) Assimilation of cholesterol by some cultures of lactic acid bacteria and bifidobacteria.Biotechnol. Lett. 14: 39–44.CrossRefGoogle Scholar
  8. [8]
    Halliwell, B. (1994) Free radicals and antioxidants: A personal view.Nutr. Rev. 52: 253–265.Google Scholar
  9. [9]
    Halliwell B. and S. Chirico (1993) Lipid peroxidation: Its mechanism, measurement, and significance.Am. J. Clin. Nutr. 57: 715S-725S.Google Scholar
  10. [10]
    Halliwell, B. and J. M. C. Gutterudge (1984) Oxygen toxicity, oxygen radicals, transition metals and disease.J. Biochem. 219: 1–4.Google Scholar
  11. [11]
    Jensen, M. A., J. A. Webster, and N. Straus (1993) Rapid identification of Bacteria on the basis of polymerase chain reaction-amplified ribosomal DNA spacer polymorphism.Appl. Environ. Microbiol. 59: 945–952.Google Scholar
  12. [12]
    Laguerre, G., M. R. Allard, F. Revoy, and N. Amarger (1994) Rapid identification of Rhizobia by restriction fragment length polymorphism analysis of PCR-amplified 16S rRNA genes.Appl. Environ. Microbiol. 60: 56–63.Google Scholar
  13. [13]
    Sykes, G. and F. A. Skinner (1973) Actinomycetales: Characteristics and practical technique for the isolation and characterization ofActinomyces andBifidobacteriuim species.Report of a panel discussion, In Soc. Appl. Bact. Symp. Series No. 2, pp. 327–333. Academic Press, London, UK.Google Scholar
  14. [14]
    Buch, M. L. and W. L. Porter (1952) Identification of organic acids on paper chromatograms.Anal. Chem. 24: 489–491.CrossRefGoogle Scholar
  15. [15]
    Lee, K. Y. and T. R. Heo (1998) Identification ofBifidobacterium strains at the genus level by thin layer chromatographic determination of organic acids with culture broth of isolated bacteria strain from human feces.Food Sci. Biotechnol. 7: 95–99.Google Scholar
  16. [16]
    Lin, M. Y. and F. J. Chang (2000) Antioxidative effect of intestinal bacteriaBifidobacerium longum ATCC 15708 andLactobacillus acidophilus ATCC 4356.Digestive Disease Sci. 45: 1617–1622.CrossRefGoogle Scholar
  17. [17]
    Shimada K., K. Fujikawa, K. Yahara, and T. Nakamura (19912) Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion.J. Agric. Food Chem. 40: 945–948.Google Scholar

Copyright information

© The Korean Society for Biotechnology and Bioengineering 2003

Authors and Affiliations

  1. 1.Department of Biological EngineeringInha UniversityIncheonKorea

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