Immunologic Research

, 12:338 | Cite as

Major histocompatibility complex-restricted recognition of autologous chronic lymphocytic leukemia by tumor-specific T cells

  • William Sherman
  • Zhouru Liu
  • Giorgio Inghirami
  • Elaine F. Reed
  • Paul E. Harris
  • Nicole M. Suciu-Foca
Article

Abstract

From the peripheral blood of a patient with chronic lymphocytic leukemia (CLL) we generated a T-cell line and clones which recognized autologous CLL. The line comprised T-cell clones which responded to the CLL as well as to autologous Epstein-Barr virus (EBV)-transformed B cells in an HLA-DR-restricted fashion. In addition, the line comprised clones which were CLL-sepcific and showed no reactivity against EBV-transformed B cells and against autologous peripheral blood mononuclear cells obtained during remission. The proliferative response of the CLL-specific T-cell clone was inhibited by monoclonal antibodies to HLA-DR11, the major histocompatibility complex (MHC)-restrictive element. These results indicate that the MHC class-II molecule of CLL binds a tumor-specific peptide which is recognized by autologous T cells in an MHC class-II-restricted fashion. Such a peptide may serve as a target for immunotherapy.

Key words

Chronic lymphocytic leukemia Tumor-specific antigen MHC-restricted recognition 
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Copyright information

© Humana Press Inc. 1993

Authors and Affiliations

  • William Sherman
    • 1
  • Zhouru Liu
    • 1
  • Giorgio Inghirami
    • 1
  • Elaine F. Reed
    • 1
  • Paul E. Harris
    • 1
  • Nicole M. Suciu-Foca
    • 1
  1. 1.Department of Pathology, College of Physicians and SurgeonsColumbia UniversityNew YorkUSA

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