Skip to main content
SpringerLink
Log in

Effects of a herbal formula on biochemical markers of bone turnover in ovariectomlzed rats

  • Experimental work and research
  • Published:
Chinese Journal of Integrated Traditional and Western Medicine Aims and scope Submit manuscript

Abstract

Objective

To investigate the effect and mechanism of a Chinese herbal formula MG in ameliorating estrogen deficiency bone loss.

Methods

10-month old female rats were randomly divided into three groups: ovariectomized (OVX), OVX treated with MG decoction (OVX-M) and OVX treated with estrogen injection (OVX-E). The urinary pyridinoline creatinine ratio (Pyd/Cr), deoxypyridinoline creatinine ratio (Dpd/Cr), and plasma alkaline phosphatase (ALP) were determined at the baseline, 1,4,8 and 12 postoperative weeks.

Results

Our data showed an average increase of 93% in Pyd/Cr (P<0.001), 180% inDpd/Cr (P <0.005) and 59% in plasma ALP (P<0.001) respectively in the OVX group after ovariectomy. Compared with OVX rats, the increase of the OVX-M group were prevented 39% in Pyd/Cr, 102% in Dpd/Cr and 19% in ALP respectively (P<0.05).

Conclusion

The herbal formula MG possesses a therapeutic effect on estrogen dependent bone loss by inhibiting the bone turnover as estrogen replacement.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bonjour JP, Ammann P and Rizzoli R. Importance of preclinical studies in the development of drugs for treatment of osteoporosis: a review related to the 1998 WHO guidelines. Osteoporosis Int 1999; 9: 379–393.

    Article  CAS  Google Scholar 

  2. Deal CL. Osteoporosis: prevention, diagnosis, and management. Am J Med 1997; 102: 35–39.

    Article  Google Scholar 

  3. Prince RL, Smith M, Dick IM, et al. Prevention of postmenopausal osteoporosis. A comparative study of exercise, calcium supplementation, and hormone-replacement therapy. N Engl J Med 1991; 325: 1189–1195.

    PubMed  CAS  Google Scholar 

  4. Notelovitz M. Estrogen and osteoporosis: principles & practice. Am J Med Sci 1997; 313: 2–12.

    Article  PubMed  CAS  Google Scholar 

  5. Gibaldi M. Prevention and treatment of osteoporosis: does the future belong tp hormone replacement therapy? J Clin Pharmacol 1997; 37: 1087–1099.

    PubMed  CAS  Google Scholar 

  6. Colditz GA. Estrogen replacement therapy for breast cancer patients. Oncology 1997; 11: 1491–1501.

    PubMed  CAS  Google Scholar 

  7. Whitehead M and Gogfree V. Venous thrombo-embolism and hormone replacement therapy. Baillieres Clin Obstet Gynaecol 1997; 11: 587–599.

    Article  PubMed  CAS  Google Scholar 

  8. Xu M, Draper CR, Zheng MH et al. The effect of a plant extract MGJ on bone loss in the ovariectomized rats. Annual Scientific Meeting of Australian & New Zealand Bone & Mineral Society (Canberra, Australia) 1997; 7: 43.

    Google Scholar 

  9. Randall AG, Kent GN, Garcia-Webb P, et al. Comparison of biochemical markers of bone turnover in paget disease treated with pamidronate and a proposed model for the relationships between measurements of the different forms of pyridinoline cross-links. J Bone Miner Res 1996; 11: 1176–1184.

    PubMed  CAS  Google Scholar 

  10. Delmas PD, Schlemmer A, Gineyts E, et al. Urinary excretion of pyridinium crosslinks correlates with bone turnover measured on illiac crest biopsy in patients with vertebral osteoporosis. J Bone Miner Res 1991; 6: 639–644.

    Article  PubMed  CAS  Google Scholar 

  11. Prince RL. Counterpoint: estrogen effects on calcitropic hormones and calcium homeostasis. Endocrine Reviews 1994; 15: 301–309.

    Article  PubMed  CAS  Google Scholar 

  12. Kalu DN. Review: The ovariectomized rat model of postmenopausal bone loss. Bone Miner 1991; 15: 175–192.

    Article  PubMed  CAS  Google Scholar 

  13. Beardsworth LJ, Eyre DR and Dickson IR. Changes associated with age in the urinary excretion of lysyl and hydrox-ylysyl pyridinoline. Two new markers of bone collagen turnover. J Bone Miner Res 1990; 5: 671–676.

    PubMed  CAS  Google Scholar 

  14. Wronski TJ, Cintron M and Dann LM. Temporal relationship between bone loss and increased bone turnover in ovariectomized rats. Calcified Tissue International 1988; 43: 179–183.

    Article  PubMed  CAS  Google Scholar 

  15. Hoshino H, Kushida K, Takahashi M, et al. Characteristics of biochemical markers in patients with metabolic bone disorders. Endocrine Res 1988; 24: 55–64.

    Article  Google Scholar 

  16. Frolik CA, Bryant HU, Black EC, et al. Time-dependent changes in biochemical bone markers and serum cholesterol in ovariectomized rats: effects of raloxifene HCl, tamoxifen, estrogen, and alendronate. Bone 1996; 18: 621–627.

    Article  PubMed  CAS  Google Scholar 

  17. Kalu DN, Salerno E, Liu CC, et al. A comparative study of the actions of tamoxifen, estrogen and progesterone in the ovariectomized rat. Bone Miner 1991; 15: 109–124.

    Article  PubMed  CAS  Google Scholar 

  18. Dick IM, John ASt, Heal S, et al. The effect of estrogen deficiency on bone mineral density, renal calcium and phosphorus handling and calcitropic hormones in the rat. Calcif Tissue Int 1996; 59: 174–178.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Guangzhou University of Traditional Chinese Medicine, 510405, Guangzhou, PR China

    Min Xu, Jin-hua Xie, Xiao-hui Zhang & Xin-song Feng

  2. University of Western Australia, 6009, WA, Australia

    Min Xu, Kett Warren & Prince Richard

  3. Institute of Chinese Medicine, Chinese University of Hong Kong, Shatin, Hong Kong

    Min Xu

Authors
  1. Min Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jin-hua Xie
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiao-hui Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kett Warren
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Prince Richard
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Xin-song Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Min Xu.

Additional information

It is a part of the research of the Chinese-Australian Government Joint Project (1997-C12) supported by Guangdong and Guangzhou Council of Science and Technology and University of Western Australia.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, M., Xie, Jh., Zhang, Xh. et al. Effects of a herbal formula on biochemical markers of bone turnover in ovariectomlzed rats. CJIM 8, 44–47 (2002). https://doi.org/10.1007/BF02934618

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF02934618

Key Words

Search

Navigation