Pilot scale production of poly(3-hydroxybutyrate-co-3-hydroxy-valerate) by fed-batch culture of recombinantEscherichia coli
Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)], by fed-batch culture of recombinantEscherichia coli harboring a plasmid containing theAlcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only. In a 30 L fermentor having aKLa value of 0.11 s−1, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively, giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having aKLa of 0.03 s−1, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively, giving a productivity of 1.06 g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinantE. coli in a large-scale fermentor having lowKLa value.
KeywordsP(3HB/V) recombinantE. coli pilot-scale fermentor oxygen transfer rate
Unable to display preview. Download preview PDF.
- Choi, J., S. Y. Lee, and K. Han (1998) Cloning of theAlcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of the these genes for enhanced production of poly(3-hydroxybutyrate) by recombinantEscherichia coli.Appl. Environ. Microbiol. 64: 4897–4903.Google Scholar
- Choi, J. and S. Y. Lee (1999) High level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fedbatch culture of recombinantEscherichia coli.Appl. Environ. Microbiol. 65: 4363–4368.Google Scholar
- Lee, S. Y. and S. J. Park (2002) Fermentative production of SCL-PHAs. pp. 262–290. In: Y. Doi and A. Steinbüchel (eds.)Biopolymers. Vol. 3a. Wiley-VCH, Germany.Google Scholar
- Lee, S. Y. and S. J. Park (2002) Biosynthesis and fermentative production of SCL-MCL-PHAs, pp. 317–336, In: Y. Doi and A. Steinbüchel (eds.).Biopolymers, Vol. 3a. Wiley-VCH, Germany.Google Scholar
- Wong, H. H., R. J. van Wegen, J. Choi, S. Y. Lee, and A. P. J. Middelberg (1999) Metabolic analysis of poly(3-hydroxybutyrate) production by recombinantEscherichia coli.J. Microbiol. Biotechnol. 9: 593–603.Google Scholar