Biotechnology and Bioprocess Engineering

, Volume 7, Issue 6, pp 371–374

Pilot scale production of poly(3-hydroxybutyrate-co-3-hydroxy-valerate) by fed-batch culture of recombinantEscherichia coli

  • Jong-il Choi
  • Sang Yup Lee
  • Kyungsup Shin
  • Woo Gi Lee
  • Si Jae Park
  • Ho Nam Chang
  • Yong Keun Chang
Article

Abstract

Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB/V)], by fed-batch culture of recombinantEscherichia coli harboring a plasmid containing theAlcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes, was examined in two pilot-scale fermentors with air supply only. In a 30 L fermentor having aKLa value of 0.11 s−1, the final P(3HB/V) concentration and the P(3HB/V) content obtained were 29.6 g/L and 70.1 wt%, respectively, giving a productivity of 1.37 g P(3HB/V)/L-h. In a 300 L fermentor having aKLa of 0.03 s−1, the P(3HB/V) concentration and the P(3HB/V) content were 20.4 g/L and 69 wt%, respectively, giving a productivity of 1.06 g P(3HB/V)/L-h. These results suggest that economical production of P(3HB/V) is possible by fed-batch culture of recombinantE. coli in a large-scale fermentor having lowKLa value.

Keywords

P(3HB/V) recombinantE. coli pilot-scale fermentor oxygen transfer rate 

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References

  1. [1]
    Lee, S. Y. (1996) Bacterial polyhydroxyalkanoates,Biotechnol. Bioeng. 49: 1–14.CrossRefGoogle Scholar
  2. [2]
    Steinbüchel, A. and B. Füchtenbusch (1998) Bacterial and other biological systems for polyester production.Trends Biotechnol. 16: 419–427.CrossRefGoogle Scholar
  3. [3]
    Lee, S. Y. (1996) Plastic bacteria? Progress and prospects for polyhydroxyalkanoate production in bacteria.Trends Biotechnol. 14: 431–438.CrossRefGoogle Scholar
  4. [4]
    Choi, J. and S. Y. Lee (1997) Process analysis and economic evaluation for poly(3-hydroxybutyrate) production by fermentation.Bioprocess Eng. 17: 335–342.CrossRefGoogle Scholar
  5. [5]
    Choi, J., S. Y. Lee, and K. Han (1998) Cloning of theAlcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of the these genes for enhanced production of poly(3-hydroxybutyrate) by recombinantEscherichia coli.Appl. Environ. Microbiol. 64: 4897–4903.Google Scholar
  6. [6]
    Choi, J. and S. Y. Lee (1999) High level production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by fedbatch culture of recombinantEscherichia coli.Appl. Environ. Microbiol. 65: 4363–4368.Google Scholar
  7. [7]
    Braunegg, G., B. Sonnleitner, and R. M. Lafferty (1978) A rapid gas chromatographic method for the determination of poly-β-hydroxybutyric acid in microbial biomass.Eur. J. Appl. Microbiol. Biotechnol. 6: 29–37.CrossRefGoogle Scholar
  8. [8]
    Chang, H. N., B. Halard, and M.-Y. Murray (1989) Measurement ofK La by a gassing-in method with oxygenenriched air.Biotechnol. Bioeng. 34: 1147–1157.CrossRefGoogle Scholar
  9. [9]
    Lee, S. Y. and S. J. Park (2002) Fermentative production of SCL-PHAs. pp. 262–290. In: Y. Doi and A. Steinbüchel (eds.)Biopolymers. Vol. 3a. Wiley-VCH, Germany.Google Scholar
  10. [10]
    Lee, S. Y. and S. J. Park (2002) Biosynthesis and fermentative production of SCL-MCL-PHAs, pp. 317–336, In: Y. Doi and A. Steinbüchel (eds.).Biopolymers, Vol. 3a. Wiley-VCH, Germany.Google Scholar
  11. [11]
    Mehta, V. D. and M. M. Sharma (1971) Mass transfer in mechanically agitated gas-liquid contactors.Chem. Eng. Sci. 26: 461–479.CrossRefGoogle Scholar
  12. [12]
    van Wegen, R. J., S. Y. Lee and A. P. J. Middelberg (2001) Metabolic and kinetic analysis of poly(3-hydroxybutyrate) production by recombinantEscherichia coli.Biotechnol. Bioeng. 74: 70–80.CrossRefGoogle Scholar
  13. [13]
    Wong, H. H., R. J. van Wegen, J. Choi, S. Y. Lee, and A. P. J. Middelberg (1999) Metabolic analysis of poly(3-hydroxybutyrate) production by recombinantEscherichia coli.J. Microbiol. Biotechnol. 9: 593–603.Google Scholar
  14. [14]
    Park, S. J., J. P. Park, and S. Y. Lee (2002) Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinantEscherichia coli in a pilot-scale fermenter.Biotechnol. Lett. 24: 185–189.CrossRefGoogle Scholar

Copyright information

© The Korean Society for Biotechnology and Bioengineering 2002

Authors and Affiliations

  • Jong-il Choi
    • 1
    • 2
  • Sang Yup Lee
    • 1
    • 2
    • 3
  • Kyungsup Shin
    • 2
  • Woo Gi Lee
    • 2
  • Si Jae Park
    • 1
    • 2
  • Ho Nam Chang
    • 2
  • Yong Keun Chang
    • 2
  1. 1.Metabolic and Biomolecular Engineering National Research LaboratoryKorea Advanced Institute of Science and TechnologyDaejeonKorea
  2. 2.Department of Chemical and Biomolecular Engineering and BioProcess Engineering Research CenterKorea Advanced Institute of Science and TechnologyDaejeonKorea
  3. 3.Center for Ultramicrochemical Process SystemsKorea Advanced Institute of Science and TechnologyDaejeonKorea

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