Applied Biochemistry and Biotechnology

, Volume 51, Issue 1, pp 413–422 | Cite as

Improvement of productivity in acetic acid fermentation withClostridium thermoaceticum

  • Minish M. Shah
  • Munir Cheryan
Session 3 Bioprocessing Research


Production of acetic acid by a mutant strain ofClostridium thermoaceticum was compared in three types of membrane cell-recycle bioreactors. A modified fed-batch bioreactor (where the product is partially removed at the end of fermentation, but the cells are retained), and a two-stage CSTR (with product being removed continuously and the cells being recycled from the second to the first stage) resulted in better performance than a one-stage CSTR or batch fermenter. The difference in performance was greater at higher acetate concentration. With 45 g/L of glucose in the feed, productivity was 0.75–1.12 g/L·h and acetic acid concentrations were 34–38 g/L. This is more than double the batch system. The nutrient supply rate also appeared to have a strong influence on productivity of the microorganism.

Index Entries

clostridium thermoaceticum calcium magnesium acetate membrane bioreactor acetic acid fermentation 


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Copyright information

© Humana Press Inc. 1995

Authors and Affiliations

  • Minish M. Shah
    • 1
  • Munir Cheryan
    • 1
  1. 1.Agricultural Bioprocess LaboratoryUniversity of IllinoisUrbana

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