Production of mannitol usingLeuconostoc mesenteroides NRRL B-1149
- 114 Downloads
A process for the production of mannitol from fructose (5% to 25%) usingLeuconostoc mesenteroides NRRL B-1149 was investigated. Fermentations were carried out in batch or fed-batch fermentations without aeration at 28°C, pH 5.0. When 5% fructose was used in batch culture fermentation, the yield of mannitol was 78% of that expected theoretically. When the fructose concentration was increased to 10%, the yield dropped to 59.6% of the theoretical value. However, in the fed-batch culture, using 10% fructose, the yield was 81.9% of the theoretical value. In a 15% fructose fed-batch culture, with 5% fructose being added initially and the other 10% fructose being added as a continuous supply, the final yield was 83.7% of the theoretical yield. When 20% fructose was used in the same manner, the yield was 89.5% of theoretical yield.
Keywordfructose mannitol Leuconostoc mesenteroides fed-batch fermentation
Unable to display preview. Download preview PDF.
- Soetaert, W. (1996)Synthesis of d-Mannitol andl-Sorbitose by Microbial Hydrogenation and Dehydrogenation of Monosaccharide. Ph. D. Thesis. University of Gent, Gent, Belgium.Google Scholar
- Wisselink, H. W., R. A. Weusthuis, G. Eggink, J. Hugenholtz, and G. J. Grobben (2001) Mannitol production by lactic acid bacteria: A review.Int. Dairy J. 1–11.Google Scholar
- Grobben, G. J., S. W. P. G. Peters, H. W. Wisselink, R. A. Weushuis, M. H. N. Hoefnagel, J. Hugenholts, and G. Eggink (2001) Spontaneous formation of a mannitol-producing variant ofLeuconostoc mesenteroides grown in the presence of fructose.Appl. Environ. Microbiol. 67: 2876–2870.CrossRefGoogle Scholar
- Kim, D., J. S. Baek, and J. H. Lee (1995) Characteristics of dextransucrase ofLeuconostoc mesenteroides NRRL B-1299 mutants, and enzyme modification amylose and pullulan.Ann. RCNBMA 4: 41–57.Google Scholar
- Grobben, G. J., S. W. P. G. Peters, H. W. Wisselink, R. A. Weusthuis, M. H. N. Hoefnagel, J. Hugenholtz, and G. Eggink (2001) Spontaneous formation of a mannitol-producing variant ofLeuconostoc pseudomesenteroides grown in the presence of fructose.Appl. Environ. Microbiol. 66: 2867–2870.CrossRefGoogle Scholar