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Biotechnology and Bioprocess Engineering

, Volume 7, Issue 4, pp 234–236 | Cite as

Production of mannitol usingLeuconostoc mesenteroides NRRL B-1149

  • Chang Yong Kim
  • Jin Ha Lee
  • Byung Hoon Kim
  • Sun Kyun Yoo
  • Eun Seong Seo
  • Kab Su Cho
  • Donal F. Day
  • Doman Kim
Article

Abstract

A process for the production of mannitol from fructose (5% to 25%) usingLeuconostoc mesenteroides NRRL B-1149 was investigated. Fermentations were carried out in batch or fed-batch fermentations without aeration at 28°C, pH 5.0. When 5% fructose was used in batch culture fermentation, the yield of mannitol was 78% of that expected theoretically. When the fructose concentration was increased to 10%, the yield dropped to 59.6% of the theoretical value. However, in the fed-batch culture, using 10% fructose, the yield was 81.9% of the theoretical value. In a 15% fructose fed-batch culture, with 5% fructose being added initially and the other 10% fructose being added as a continuous supply, the final yield was 83.7% of the theoretical yield. When 20% fructose was used in the same manner, the yield was 89.5% of theoretical yield.

Keyword

fructose mannitol Leuconostoc mesenteroides fed-batch fermentation 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2002

Authors and Affiliations

  • Chang Yong Kim
    • 1
  • Jin Ha Lee
    • 2
  • Byung Hoon Kim
    • 3
  • Sun Kyun Yoo
    • 5
  • Eun Seong Seo
    • 1
  • Kab Su Cho
    • 4
  • Donal F. Day
    • 6
  • Doman Kim
    • 1
    • 2
  1. 1.Department of Material, Chemical and Biochemical EngineeringChonnam National UniversityGwangjuKorea
  2. 2.Faculty of Applied Chemical EngineeringChonnam National UniversityGwangjuKorea
  3. 3.Engineering Research InstituteChonnam National UniversityGwangjuKorea
  4. 4.Department of Molecular BiotechnologyChonnam National UniversityGwangjuKorea
  5. 5.Department of Food and BiotechnologyJoong Bu UniversityChungnamKorea
  6. 6.Audubon Sugar InstituteLouisiana State UniversityBaton RougeUSA

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