Virchows Archiv B

, Volume 43, Issue 1, pp 179–187 | Cite as

Concanavalin A distribution in polymorphonuclear leukocytes and alveolar macrophages during hyperoxia

  • M. Rister
  • M. Vollmering
Article
Received:
Accepted:
  • 10 Downloads

Summary

Hyperoxia impaires cytoskeleton-dependent phagocytic functions in polymorphonuclear leukocytes (PMNs) and alveolar macrophages (AMs). To investigate the effect of different oxygen concentrations on the cytoskeleton, in particular the microtubule (MT) and microfilament (MF) system, the fluorescence pattern of Concanavalin A (Con A) receptors in AMs and PMNs was observed. Cells were obtained from guinea-pigs exposed to different oxygen concentrations. The exposure of guinea-pigs to oxygen concentrations of up to 50% induced in AMs and PMNs mainly spontaneous Con A capping, demonstrating an altered MT system. Oxygen tensions above 50% lead to an increased number of AMs and PMNs exhibiting a patchy Con A fluorescence distribution. Even in the presence of the microtubule-disrupting agent colchicine most AMs and PMNs were unable to form a Con A cap fluorescence distribution under these high oxygen tensions. This study demonstrates that the exposure of guinea-pigs to an oxygen concentration greater than 50% increases the relative number of AMs and PMNs demonstrating a patchy distribution of Con A. This patchy fluorescence pattern is associated with a severe cytoskeletal defect, i.e. MT and MF disruption. The various leukocyte function defects induced by hyperoxia, demonstrated in previous studies, are based on this MT and MF alteration in AMs and PMNs, representing an additional sepsis-promoting factor during hyperoxia.

Key words

Polymorphonuclear leukocytes Alveolar macrophages Oxygen toxicity Cytoskeleton 

Abbreviations used in this paper

PMNs

polymorphonuclear leukocytes

AMs

alveolar macrophages

MTs

microtubules

MFs

microfilaments Con A deConcanavalin A

KRP-buffer

Krebs-Ringer-Phosphate-buffer

FiO2

fraction of inspired oxygen

GSH

reduced glutathione

SSG

oxidized glutathione

This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Autor AP, Frank L, Roberts JR (1976) Developmental characteristics of pulmonary Superoxide dismutase. Pediat Res 10:154–158PubMedCrossRefGoogle Scholar
  2. Baehner RL, Boxer LA, Allen JM, Davies J (1977) Autooxidation as a basis for altered functions of polymorphonuclear leukocytes. Blood 50:327–335PubMedGoogle Scholar
  3. Berlin RD, Oliver JM, Ukena TE, Yin HH (1975) The cell surface. New England J Med 292:515–520Google Scholar
  4. Berlin RD, Oliver JM (1978) Analogous ultrastructure and surface properties during capping and phagocytosis in leukocytes. J Cell Biol 77:789–804PubMedCrossRefGoogle Scholar
  5. Bowden DH, Adamson IYR (1974) Endothelial regeneration as a marker of the differential vascular responses in oxygen induced edema. Lab Invest 30:350–357PubMedGoogle Scholar
  6. Boxer LA, Watanabe AM, Rister M, Besch HR, Allen J, Baehner RL (1976) Correction of leukocyte function in Chediak-Higashi syndrome by ascorbate. New England J Med 296:1041–1045Google Scholar
  7. Boxer LA, Rister M, Allen J, Baehner RL (1977) Improvement of Chediak-Higashi leukocyte function by cyclic guanosine monophosphate. Blood 49:9–17PubMedGoogle Scholar
  8. Boxer LA, Allen JN, Watanabe AM, Besch HR, Baehner RL (1978) Role of microtubules in granulocyte adherence. Blood 51:1045–1050PubMedGoogle Scholar
  9. Burchill BR, Oliver JM, Pearson CB, Leinbach RD, Berlin RD (1978) Microtubule dynamics and glutathione metabolism in phagocytizing human polymorphonuclear leukocytes. J Cell Biol 76:439–447PubMedCrossRefGoogle Scholar
  10. Clark JM (1974) The toxicity of oxygen. Am Rev Resp Dis 110:40–50PubMedGoogle Scholar
  11. Crapo JD, Tierney DF (1974) Superoxide dismutase and pulmonary oxygen toxicity. Am J Physiol 226:1401–1407PubMedGoogle Scholar
  12. Fischer AD, Diamond S, Meilen S (1974) Effects of O2 exposure on metabolism of the rabbit alveolar macrophage. J Appl Physiol 37:341–345Google Scholar
  13. Fridovich I (1970) Quantitative aspects of the production of Superoxide anion radical by milk xanthine oxidase. J Biol Chem 245:4053–4057PubMedGoogle Scholar
  14. Hoffstein S, Soberman R, Goldstein I, Weissmann G (1976) Concanavalin A induces microtubule assembly and specific granule discharge in human polymorphonuclear leukocytes. J Cell Biol 68:781–787PubMedCrossRefGoogle Scholar
  15. Huber GL, La Force M, Manson R (1970a) Impairment and recovery of pulmonary antibacterial defense mechanisms after oxygen administration. J Clin Invest 49:47aGoogle Scholar
  16. Huber GL, La Force MF (1970b) Progressive impairment of pulmonary antibacterial defense mechanism associated with prolonged oxygen administration. Ann Int Med 72:808aGoogle Scholar
  17. Huber GL, Goodenough S, O’Connell D (1974) Oxygen toxicity and bacterial infection in the lung. Clin Res 22:506aGoogle Scholar
  18. Karnovsky M (1962) Metabolic basis of phagocytic activity. Physiol Rev 42:143–167PubMedGoogle Scholar
  19. Lehrer RI (1973) Effects of Colchicine and Chloramphenicol on the oxidative metabolism and phagocytic activity of human neutrophils. J Infect Dis 127:40–48PubMedGoogle Scholar
  20. Mohri H (1976) The function of tubulin in motile systems. Biochem Biophys Acta 456:85–127PubMedGoogle Scholar
  21. Murphey SA, Hyams JS, Fischer AB, Root RK (1975) Effects of oxygen exposure on in vitro function of pulmonary alveolar macrophages. J Clin Invest 56:503–511PubMedCrossRefGoogle Scholar
  22. Nicolson GL, Poste G (1976) The cancer cell: Dynamic aspects and modifications in cell surface organization. New England J Med 195:197–203Google Scholar
  23. Northway WH, Rezau L, Petriceks R, Besch K (1976) Oxygen toxicity in the newborn lung. Reversal of inhibition of DNA synthesis in the mouse. Pediatrics 57:41–46PubMedGoogle Scholar
  24. Oliver JM, Ukena TE, Berlin RD (1974) Effects of phagocytosis and Colchicine on the distribution of lectinbinding sites on cell surfaces. Proc Natl Acad Sci USA 71:394–398PubMedCrossRefGoogle Scholar
  25. Oliver JM, Zurier RB, Berlin RD (1975a) Concanavalin A cap formation on polymorphonuclear leukocytes of normal and beige (Chediak-Hiagashi) mice. Nature 253:471–473PubMedCrossRefGoogle Scholar
  26. Oliver JM (1975b) Microtubules, cyclic GMP and control of cell surface topography. Immune recognition. Academic Press, New York, pp 445–471.Google Scholar
  27. Oliver JM, Albertini DF, Berlin RD (1976a) Effects of glutathione oxidizing agents on microtubule assembly and microtubule-dependent surface properties of human neutrophils. J Cell Biol 71:921–932PubMedCrossRefGoogle Scholar
  28. Oliver JM, Zurier RB (1976b) Correction of characteristic abnormalities of microtubule function and granule morphology in Chediak-Higashi syndrome. J Clin Invest 57:1239–1247PubMedCrossRefGoogle Scholar
  29. Oliver JM (1976c) Impaired microtubule function correctable by cyclic GMP and cholinergic agonists in the Chediak-Higashi syndrome. Am J Pathol 85:395–418PubMedGoogle Scholar
  30. Oliver JM, Berlin RD, Baehner RL, Boxer LA (1977) Mechanism of microtubule disassembly in vivo: Studies in normal and chronic granulomatous disease leukocytes. Br J Haem 37:311–322CrossRefGoogle Scholar
  31. Oliver JM Spielberg SP, Pearson CB, Schulman JD (1978) Microtubule assembly and function in normal and glutathione synthetase deficient polymorphonuclear leukocytes. J Immunol 120:1181–1186PubMedGoogle Scholar
  32. Oren R, Farhan AE, Saito K, Mikofsky E, Karnovsky ML (1963) Metabolic patterns in three types of phagocytizing cells. J Cell Biol 17:487–501PubMedCrossRefGoogle Scholar
  33. Poste G, Papahdiopopulos D, Jacobson K, Vail WJ (1975) Effects of local anesthetics on membrane properties. Biochem Biophys Acta 394:520–539PubMedCrossRefGoogle Scholar
  34. Pratt PC (1974) Pathology of pulmonary oxygen toxicity. Am Rev Resp Dis110:51–57PubMedGoogle Scholar
  35. Resnick JS, Brown DM, Wenier RL (1974) Oxygen toxicity in fetal organ culture. II The developing lung. Lab Invest 31:665–677Google Scholar
  36. Rister M, Baehner RL (1976) The alteration of Superoxide dismutase, catalase, glutathione peroxidase, and NAD(P)H cytochrome C reductase in guniea-pig polymorphonuclear leukocytes and alveolar macrophages during hyperoxia. J Clin Invest 58:1174–1184PubMedCrossRefGoogle Scholar
  37. Rister M, Baehner RL (1977) Effects of hyperoxia on Superoxide anion and hydrogen peroxide production of polymorphonuclear leukocytes and alveolar macrophages Br J Haemat 36:241–248CrossRefGoogle Scholar
  38. Rister M, Baehner RL (1978) Neue Aspekte zur Toxizität des Sauerstoffs. Dtsch Med Wochenschr 103:257–267CrossRefGoogle Scholar
  39. Rister M (1980a) Phagocytosemechanismen alveolärer Makrophagen und Granulozyten. Blut 41:257–267PubMedCrossRefGoogle Scholar
  40. Rister M, Brucke G, Wegener F, Gladtke E (1980b) Concanavalin A capping in polymorphonuclear leukocytes. Eur J Ped 135:153–160CrossRefGoogle Scholar
  41. Rister M, Haneke C (1980c) Therapie des Steinbrinck-Chediak-Higashi Syndroms. Klin Pädiat 192:19–24CrossRefGoogle Scholar
  42. Rister M (1982) Effects of hyperoxia on phagocytosis. Blut 45:157–166PubMedCrossRefGoogle Scholar
  43. Ukena TE, Berlin RD (1972) Effect of colchicine and vinblastine on the topographical separation of membrane functions. J Exp Med 136:1–7PubMedCrossRefGoogle Scholar
  44. Williams D, Boxer LA, Oliver JM, Baehner RL (1977) Cytoskeleton regulation of Concanavalin A capping in pulmonary alveolar macrophage. Nature 267:255–257PubMedCrossRefGoogle Scholar
  45. Wilson L, Bamberg JR (1974) Interaction of drugs with microtubule proteins. Fed Proc 33:158–166PubMedGoogle Scholar
  46. Winter PM, Smith G (1972) The toxicity of oxygen. Anesthesist 37:210–241CrossRefGoogle Scholar
  47. Zigmond SH, Hirsch JG (1973) Leukocyte locomotion and chemotaxis. J Exp Med 137:9CrossRefGoogle Scholar
  48. Zurier RB, Weissmann G, Hoffstein S, Kammerman S, Tai HH (1974) Mechanism of lysosomal enzyme release from human leukocytes. Clin Invest 53:297–309CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • M. Rister
    • 1
  • M. Vollmering
    • 1
  1. 1.Universitäts-Kinderklinik KölnKöln 41Federal Republic of Germany

Personalised recommendations