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Morphological diversity ofMortierella alpina: Effect of consumed carbon to nitrogen ratio in flask culture

  • Enoch Y. Park
  • Yasuhisa Koike
  • Hong Jie Cai
  • Kenichi Higashiyama
  • Shigeaki Fujikawa
Article

Abstract

The influence of the consumed carbon to nitrogen (C/N) ratio on mycelial morphology was investigated in cultures ofMortierella alpina using shake flasks. The consumed C/N ratio was varied from 5 to 32 under the condition that the total initial amount of the carbon and nitrogen sources was 50 g/L. The whole mycelia and filamentous mycelia exhibited no relationship with the consumed C/N ratio below a consumed C/N ratio of 20 in the presence of either excess carbon or excess nitrogen. However, when the consumed C/N ratio increased higher than 20, the mycelial sizes increased in proportion to the consumed C/N ratio. However, the area ratio of filamentous mycelia to total mycelia was found to be independent of the consumed C/N ratio, and remained constant at 0.82. In the case of a fixed consumed C/N ratio of 20, the whole mycelia and filamentous mycelia increased in proportion to the degree of the medium strength, yet the area ratio of filamentous mycelia to total mycelia remained unchanged at 0.76. Accordingly, these results show that fungal morphology and mycelial size are both affected by the ratio of carbon to nitrogen. The findings of the current study will be helpful in obtaining the efficient production of useful bioproducts from fungal cultures.

Keywords

Mortierella alpina mycelial morphology consumed C/N ratio 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2001

Authors and Affiliations

  • Enoch Y. Park
    • 1
  • Yasuhisa Koike
    • 1
  • Hong Jie Cai
    • 1
  • Kenichi Higashiyama
    • 2
  • Shigeaki Fujikawa
    • 2
  1. 1.Laboratory of Biotechnology, Department of Applied Biological Chemistry, Faculty of AgricultureShizuoka UniversityShizuokaJapan
  2. 2.Institute for Fundamental ResearchSuntory Ltd.OsakaJapan

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