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Biotechnology and Bioprocess Engineering

, Volume 6, Issue 6, pp 419–425 | Cite as

Fabrication and characterisation of a novel pellicular adsorbent customised for the effective fluidised bed adsorption of protein products

  • Yan Sun
  • Andrzej W. Pacek
  • Alvin W. Nienow
  • Andrew LyddiattEmail author
Article

Abstract

A dense pellicular solid matrix has been fabricated by coating 4% agarose gel on to dense zirconia-silica (ZS) spheres by water-in-oil emulsification. The agarose evenly laminated the ZS bead to a depth of 30 μm, and the resulting pellicular assembly was characterised by densities up to 2.39 g/mL and a mean particle diameter of 136 μm. In comparative fluidisation tests, the pellicular solid phase exhibited a two-fold greater flow velocity than commercial benchmark adsorbents necessary to achieve common values of bed expansion. Furthermore, the pellicular particles were characterised by improved qualities of chromatographic behaviour, particularly with respect to a three-fold increase in the apparent effective diffusivity of lysozyme within a pellicular assembly modified with Cibacron Blue 3GA. The properties of rapid protein adsorption/desorption were attributed to the physical design and pellicular deployment of the reactive surfaces in the solid phase. When combined with enhanced feedstock throughput, such practical advantages recommend the pellicular assembly as a base matrix for the selective recovery of protein products from complex, particulate feedstocks (whole fermentation broths, cell disruptates and biological extracts).

Keywords

pellicular adsorbent adsorption equilibrium adsorption kinetics fluidised bed hydrodynamics 

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Copyright information

© The Korean Society for Biotechnology and Bioengineering 2001

Authors and Affiliations

  • Yan Sun
    • 2
  • Andrzej W. Pacek
    • 1
  • Alvin W. Nienow
    • 1
  • Andrew Lyddiatt
    • 1
    Email author
  1. 1.Biochemical Recovery Group, Centre for Formulation Engineering, School of Engineering (Chemical Engineering)University of BirminghamBirminghamUK
  2. 2.Department of Biochemical EngineeringTianjin UniversityTianjinP. R. China

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