Fabrication and characterisation of a novel pellicular adsorbent customised for the effective fluidised bed adsorption of protein products
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A dense pellicular solid matrix has been fabricated by coating 4% agarose gel on to dense zirconia-silica (ZS) spheres by water-in-oil emulsification. The agarose evenly laminated the ZS bead to a depth of 30 μm, and the resulting pellicular assembly was characterised by densities up to 2.39 g/mL and a mean particle diameter of 136 μm. In comparative fluidisation tests, the pellicular solid phase exhibited a two-fold greater flow velocity than commercial benchmark adsorbents necessary to achieve common values of bed expansion. Furthermore, the pellicular particles were characterised by improved qualities of chromatographic behaviour, particularly with respect to a three-fold increase in the apparent effective diffusivity of lysozyme within a pellicular assembly modified with Cibacron Blue 3GA. The properties of rapid protein adsorption/desorption were attributed to the physical design and pellicular deployment of the reactive surfaces in the solid phase. When combined with enhanced feedstock throughput, such practical advantages recommend the pellicular assembly as a base matrix for the selective recovery of protein products from complex, particulate feedstocks (whole fermentation broths, cell disruptates and biological extracts).
Keywordspellicular adsorbent adsorption equilibrium adsorption kinetics fluidised bed hydrodynamics
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- Wells, C. M., K. Patel, and A. Lyddiatt (1987) Liquid fluidised bed adsorption. pp. 200–209. In: M. J. Verrall and M. J. Hudson (eds.):Separations for Biotechnology: Ellis-Horwood, Chichester, UK.Google Scholar
- Draeger N. M., and H. A. Chase (1990) Liquid fluidised bed adsorption in the presence of cells.Bioseparation 2: 67–80.Google Scholar
- Gilchrist G. R., M. T. Burns, and A. Lyddiatt (1994) Solid phases for protein adsorption in fluidised beds: Comparison of commercial and custom assembled particles. pp. 186–192. In: M. J. Verrall and M. J. Hudson (eds.),Separations for Biotechnology: Ellis-Horwood, Chichester, UK.Google Scholar
- Zhu J., A. Lyddiatt, A. W. Pacek, and A. W. Nienow (1997) Fabrication and characterisation of agar/zircon sand composite adsorbents for protein recovery in liquid fluidised beds. pp 103–114. In: Proceedings Fourth International Conference on Bioreactor and Bioprocess Fluid Dynamics. BHR Group/Inst. Mechanical Engineering Publications, London, UK.Google Scholar
- Gibson, N. B. (1991).Liquid Fluidised Bed Adsorption in Protein Recovery: Assembly and Characterisation of Dedicated Solid Phases. PhD thesis, University of Birmingham, UK.Google Scholar
- Gibson, N. B., and A. Lyddiatt (1993) Cellulose composites in liquid fluidised bed adsorption and recovery of proteins. pp. 55–62. In: J. F. Kennedy, G. O. Phillips and P. A. Williams (eds.).Cellulosics Materials for Selective Separations and Other Technologies, Ellis-Horwood, Chichester, UKGoogle Scholar
- Levenspiel, O. (1999)Chemical Reaction Engineering, 3rd ed, John Wiley & Sons, New York, USA.Google Scholar
- Bird, B., W. E. Stewart, and I. Lighfoot (1960)Transport Phenomena, Wiley and Sons, NY, USA.Google Scholar
- Geankopolis C. J. (1983)Transport Processes and Unit Operations. 2nd ed. Allyn and Bacon, NY, USA.Google Scholar
- Richardson, J. F. and W. N. Zaki (1954) Sedimentation and fluidization: Part I.Trans. Inst. Chem. Eng. 32: 35–53.Google Scholar