Folia Microbiologica

, 52:86 | Cite as

Effect of environmental factors on biofilm formation by clinicalStenotrophomonas maltophilia isolates

  • G. Di BonaventuraEmail author
  • S. Stepanović
  • C. Picciani
  • A. Pompilio
  • R. Piccolomini


The influence of environmental factors (temperature, aerobiosis-anaerobiosis, static-dynamic conditions, pH) was determined on biofilm formation by 51S. maltophilia clinical isolates. The strains produced more biofilm at 32 °C than at 37 or 18 °C. Aerobic and 6 % CO2 atmosphere yielded comparable biofilm amounts, higher than under anaerobic conditions. Biofilm production was not affected by staticvs. agitated culture conditions. Biofilm production at pH 7.5 and 8.5 was comparable but significantly higher than at pH 5.5. The capacity of individual strains to form biofilm and thus contribute to the severity of some diseases is influenced by host traits and environmental conditions at the site of infection, and play an important role in the pathogenesis of biomaterial-related disease caused byS. maltophilia.


Stenotrophomonas Maltophilia Brain Heart Infusion Broth Cystic Fibrosis Research Italian Cystic Fibrosis Agitate Culture Condition 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. de Abreu Vidipò L., de Andrade Marques E., Puchelle E., Plotkowski M.C.:Stenotrophomonas maltophilia interaction with human epithelial respiratory cellsin vitro.Microbiol.Immunol. 45, 563–569 (2001).Google Scholar
  2. Biswas S.K., Chaffin W.L.: Anaerobic growth ofCandida albicans does not support biofilm formation under similar conditions used for aerobic biofilm.Curr.Microbiol. 51, 100–104 (2005).PubMedCrossRefGoogle Scholar
  3. Černohorská L., Votava M.: Determination of minimal regrowth concentration (MRC) in clinical isolates of various biofilm-forming bacteria.Folia Microbiol. 49, 75–78 (2004).CrossRefGoogle Scholar
  4. Costerton J.W., Stewart P.S., Greenberg E.P.: Bacterial biofilms: a common cause of persistence infections.Science 284, 1318–1322 (1999).PubMedCrossRefGoogle Scholar
  5. Dancer S.J.: Mopping up hospital infection.J.Hosp.Infect. 43, 85–100 (1999).PubMedCrossRefGoogle Scholar
  6. Denton M., Kerr K.G.: Microbiological and clinical aspects of infection associated withStenotrophomonas maltophilia.Clin.Microbiol.Rev. 11, 57–80 (1998).PubMedGoogle Scholar
  7. Di Bonaventura G., Spedicato I., d’Antonio D., Robuffo I., Piccolomini R.: Biofilm formation byStenotrophomonas maltophilia: modulation by quinolones, trimethoprim-sulfamethoxazole, and ceftazidime.Antimicrob.Agents Chemother. 48, 151–160 (2004).PubMedCrossRefGoogle Scholar
  8. Donaldson S.H., Boucher R.C.: Update on pathogenesis of cystic fibrosis lung disease.Curr.Opin.Pulm.Med. 9, 486–491 (2003).PubMedCrossRefGoogle Scholar
  9. Guiney D.G.: Regulation of bacterial virulence gene expression by the host environment.J.Clin.Invest. 99, 565–569 (1997).PubMedCrossRefGoogle Scholar
  10. Harjai K., Khandwaha R.K., Mittal R., Yadav V., Gupta V., Sharma S.: Effect of pH on production of virulence factors by biofilm cells ofPseudomonas aeruginosa.Folia Microbiol. 50, 99–102 (2005).CrossRefGoogle Scholar
  11. Jucker B.A., Harms H., Zehnder A.J.: Adherence of the positively charged bacteriumStenotrophomonas (Xanthomonas) maltophilia 70401 to glass and teflon.J.Bacteriol. 178, 5472–5479 (1996).PubMedGoogle Scholar
  12. Kellum J.A.: Determinants of blood pH in health and disease.Crit.Care 4, 6–14 (2000).PubMedCrossRefGoogle Scholar
  13. Oie S., Kamiya A.: Microbial contamination of antiseptics and disinfectants.Am.J.Infect.Control 24, 389–395 (1996).PubMedCrossRefGoogle Scholar
  14. Růžička F., Holá V., Votava M., Tejkalová R., Horvát R., Heroldová M., Woznicová V.: Biofilm detection and the clinical significance ofStaphylococcus epidermidis isolates.Folia Microbiol. 49, 596–600 (2004).CrossRefGoogle Scholar
  15. Senol E., Desjardin J., Stark P.C., Barefoot L., Snydman D.R.: Attributable mortality toStenotrophomonas maltophilia bacteremia.Clin.Infect.Dis. 34, 1653–1656 (2002).PubMedCrossRefGoogle Scholar
  16. Squier C., Yu V.L., Stout J.E.: Waterborne nosocomial infections.Curr.Infect.Dis.Rep. 6, 490–496 (2000).CrossRefGoogle Scholar
  17. Stepanović S., Vuković D., Ježek P., Pavlović M., Švabić-Vlahović M.: Influence of dynamic conditions on biofilm formation by staphylococci.Eur.J.Clin.Microbiol.Infect.Dis. 20, 502–504 (2001).PubMedCrossRefGoogle Scholar
  18. Stepanović S., Ćirković I., Mijać V., Svabić-Vlahović M.: Influence of the incubation temperature, atmosphere and dynamic conditions on biofilm formation bySalmonella spp.J.Food Microbiol. 20, 339–343 (2003a).CrossRefGoogle Scholar
  19. Stepanović S., Djukić N., Djordjević V., Djukić S.: Influence of the incubation atmosphere on the production of biofilm by staphylococci.Clin.Microbiol.Infectol. 9, 955–958 (2003b).CrossRefGoogle Scholar
  20. Svabić-Vlahović M., Dakić I., Vuković D., Stepanović S.: Biofilm production byStaphylococcus epidermidis at 37 and 32 °C. Abstr. P926,13th Eur. Congr. Clin. Microbiol. Infect. Dis., Glasgow (UK) 2003.Google Scholar
  21. Van Couwenberghe C., Cohen S.: Analysis of epidemic and endemic isolates ofXanthomonas maltophilia by contour clamped homogeneous electric field gel electrophoresis.Infect.Control Hosp.Epidemiol. 15, 691–696 (1994).CrossRefGoogle Scholar
  22. Walencka E., Sadowska B., Różalska S., Hryniewicz W., Różalska B.:Staphylococcus aureus biofilm as a target for single or repeated doses of oxacillin, vancomycin, linezolid and/or lysostaphin.Folia Microbiol. 51, 381–386 (2006).CrossRefGoogle Scholar
  23. Woodard L.M., Bielkie A.R., Eisses J.F., Ketchum P.A.: Occurrence of nitrate reductase and molybdopterin inXanthomonas maltophilia.Appl.Environ.Microbiol. 56, 3766–3771 (1990).PubMedGoogle Scholar
  24. Worlitzsch D., Tarran R., Ulrich M., Schwab U., Cekici A., Meyer K.C., Birrer, P., Bellon G., Berger J.: Effect of reduced mucus oxygen concentration in airwayPseudomonas infections of cystic fibrosis patients.J.Clin.Invest. 109, 317–325 (2002).PubMedGoogle Scholar

Copyright information

© Institute of Microbiology, Academy of Sciences of the Czech Republic 2007

Authors and Affiliations

  • G. Di Bonaventura
    • 1
    • 2
    Email author
  • S. Stepanović
    • 3
  • C. Picciani
    • 1
    • 2
  • A. Pompilio
    • 1
    • 2
  • R. Piccolomini
    • 1
    • 2
  1. 1.Department of Biomedical Sciences, Faculty of MedicineGabriele d’Annunzio UniversityChietiItaly
  2. 2.Aging Research Center, Centro per lo Studio dell’InvecchiamentoGabriele d’Annunzio University FoundationChietiItaly
  3. 3.Department of BacteriologyInstitute of Microbiology and Immunology, School of MedicineBelgradeSerbia

Personalised recommendations